Using nucleotide sequencing to determine HBV genotypes in Kerman
Materials and Methods: In this cross-sectional study, twenty-one specimens were collected from blood samples available in the medical diagnostic laboratories of Kerman province during one year. After DNA extraction, PCR was carried on by specific primers, then they were sequenced. The obtained sequenced were compared with sequences in the NCBI gene bank and blasted for identification of their genotypes.
Results: Seven samples from twenty one samples (33.3%) had D genotype, 13 samples from 21 (62%) had D3 subgenotype and 1 sample from 21 (4.7%) had D4 subgenotype.
Conclusion: The prevalence of these genotypes in the Kermanian patients that recognized in this study can help to provide diagnostic kits for hepatitis B virus.
Keywords: Hepatitis B virus, genotype, PCR, Sequencing, NCBI.
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