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The Inhibitory Effects of Lactobacillus Reuteri’s Cell Wall on Cell Proliferation in the HCT-116 Colorectal Cancer Cell Line

Zahra Rafiei Atani, Ebrahim Faghihloo, Zohreh Ghalavand, Gita Eslami




Background: Colorectal cancer (CRC) is the third and a second common cancer in men and women respectively in the world and about 1.4 million new cases diagnosed in 2012. The normal gut microflora consists of bacterial species. One group of them is probiotics, which confer a health benefit to the host. Lactobacillus reuteri (L.reuteri) is known as a probiotic, which lead to the prevention of colorectal cancer. The aim of this study was to assess the inhibitory effects of Lactobacillus reuteri’s cell wall on cell proliferation in the colorectal cancer HCT-116 cell line.

Materials and Methods: The cells of HCT-116 cell line were grown at 37ᵒC, 5% CO2. L.reuteri was obtained from the Iranian Biological Resource Center and cultured in the MRS Broth at 37ᵒC for 48h anaerobically. The cell wall was prepared by the freezing-thawing procedure. So the inhibitory effect of L.reuteri on the growth and proliferation of HCT-116 cells was assessed by MTT assay.

Results: The cell wall from L.reuteri inhibited cell proliferation on colorectal cancer HCT-116 cell line. It showed dose- and –time dependent inhibition.

Conclusion: These results demonstrated that cell wall of L.reuteri inhibits cell proliferation of HCT-116 cell line.


Colorectal cancer, Lactobacillus reuteri, HCT-116 cell line


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DOI: https://doi.org/10.22037/nbm.v6i3.19415