Seroprevalence of Toxocara infection among healthy individuals referred to the medical center laboratories in Tehran City, Capital of Iran

Zahra Baghani, Hooshang Khazan, Mohammad Reza Sohrabi, Ali Rostami

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Abstract

Background: Toxocarosis is a zoonotic disease with worldwide distribution. Humans’ infection occurred by incidental ingestion of eggs shed in feces of dogs or cats. Studies on general population are rare in Iran. In this cross-sectional study, we investigated seroprevalence, and risk factors associated with toxocariasis among the healthy individuals in Tehran, capital if Iran.

Materials and Methods: In total, 374 sera samples were investigated for the presence of anti-Toxocara IgG. We applied ELISA as screening test using available commercial kit. In addition, demographic data were obtained from participant’s questionnaires. Data analysis was performed using SPSS16.

Results: The overall seroprevalence of toxocariasis was found 5.6% (21/374). Regarding the socio-demographic variables, age (P<0.001) and eating unwashed vegetables (P=0.049) were significantly associated to toxocariasis in univariate analysis. In the logistic regression analysis, only age (P<0.001) was identified as potential risk factor associated with Toxocara infection.

Conclusion: This study revealed that seroprevalence of toxocariasis is relatively low in the healthy individuals in Tehran. We suggest carrying out further studies in the different part of Iran and investigate on the prevalence of toxocariasis in high-risk groups such as asthma, hyper-eosinophilic, epilepsy, rheumatism and schizophrenia patients.

Keywords

Toxocariasis, Seroprevalence, Tehran, Iran

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References

Smith H, Holland C, Taylor M, Magnaval J, Schantz P, Maizels R. How common is human toxocariasis? Towards standardizing our knowledge. Trend Parasitol. 2009;25(4):182-8.

Overgaauw PA, van Knapen F. Veterinary and public health aspects of Toxocara spp. Vet Parasitol. 2013;193(4):398-403.

Jenkins EJ, Castrodale LJ, de Rosemond S, Dixon BR, Elmore SA, Gesy KM, et al. Tradition and transition: parasitic zoonoses of people and animals in Alaska, northern Canada, and Greenland. Adv Parasitol. 2013;82:33-204.

Choi D, Lim JH, Choi D-C, Lee KS, Paik SW, Kim SH, et al. Transmission of Toxocara canis via ingestion of raw cow liver: a cross-sectional study in healthy adults. Korean J Parasitol. 2012;50(1):23-7.

Rostami A, Ebrahimi M, Mehravar S, Omrani VF, Fallahi S, Behniafar H. Contamination of commonly consumed raw vegetables with soil transmitted helminth eggs in Mazandaran province, northern Iran. Int J Food Microbiol. 2016;225:54-8.

Siyadatpanah A, Tabatabaei F, Zeydi AE, Spotin A, Omrani VF, Assadi M, et al. Parasitic contamination of raw vegetables in Amol, North of Iran. Arch Clin Infect Dis. 2013;8(2): e15983.

Despommier D. Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev. 2003;16(2):265-72.

Macpherson CN. The epidemiology and public health importance of toxocariasis: a zoonosis of global importance. Int J Parasitol. 2013;43(12):999-1008.

Ma G, Holland CV, Wang T, Hofmann A, Fan C-K, Maizels RM, et al. Human toxocariasis. Lancet Infect Dis. 2017. http://dx.doi.org/10.1016/ S1473-3099(17)30331-6

Fragoso RP, Monteiro MBM, Lemos EM, Pereira FEL. Anti-Toxocara antibodies detected in children attending elementary school in Vitoria, State of Espírito Santo, Brazil: prevalence and associated factors. Rev Soc Bras Med Trop. 2011;44(4):461-6.

Gavignet B, Piarroux R, Aubin F, Millon L, Humbert P. Cutaneous manifestations of human toxocariasis. J Am Acad Dermatol 2008;59 (6):1031-42.

Kwon N-H, Oh M-J, Lee S-P, Lee B-J, Choi D-C. The prevalence and diagnostic value of toxocariasis in unknown eosinophilia. Ann Hematol. 2006;85(4):233-8.

Fillaux J, Magnaval J-F. Laboratory diagnosis of human toxocariasis. Vet Parasitol. 2013; 193(4):327-36.

Abdi J, Darabi M, Sayehmiri K. Epidemiological situation of toxocariasis in Iran: meta-analysis and systematic review. Pak J Biol Sci. 2012;15:15(22):1052-5.

Sadjjadi S, Khosravi M, Mehrabani D, Oryan A. Seroprevalence of Toxocara infection in school children in Shiraz, Southern Iran. J Trop Pediatr. 2000;46(6):327-30.

Fallah M, Azimi A, Taherkhani H. Seroprevalence of toxocariasis in children aged 1-9 years in western Islamic Republic of Iran, 2003. East Mediterr Health J. 2007;13(5):1073-7.

Roldán WH, Espinoza YA, Huapaya PE, Huiza AF, Sevilla CR, Jiménez S. Frequency of human toxocariasis in a rural population from Cajamarca, Peru determined by DOT-ELISA test. Rev Inst Med Trop Sao Paulo. 2009;51(2):67-71.

Colli CM, Rubinsky-Elefant G, Paludo ML, Falavigna DL, Guilherme EV, Mattia S, et al. Serological, clinical and epidemiological evaluation of toxocariasis in urban areas of south Brazil. Rev Inst Med Trop Sao Paulo 2010;52(2):69-74.

Won KY, Kruszon-Moran D, Schantz PM, Jones JL. National seroprevalence and risk factors for zoonotic Toxocara spp. infection. Am J Trop Med Hyg. 2008;79(4):552-7.

Lee J-Y, Yang MH, Hwang J-H, Kang M, Paeng JW, Yune S, et al. The prevalence of toxocariasis and diagnostic value of serologic tests in asymptomatic Korean adults. Allergy Asthma Immunol Res. 2015;7(5):467-75.

Gabrielli S, Tasić-Otašević S, Ignjatović A, Fraulo M, Trenkić-Božinović M, Momčilović S, et al. Seroprevalence and Risk Factors for Toxocara canis Infection in Serbia During 2015. Foodborne Pathog Dis. 2017;14(1):43-9.

Antolová D, Jarčuška P, Janičko M, Madarasová-Gecková A, Halánová M, Čisláková L, et al. Seroprevalence of human Toxocara infections in the Roma and non-Roma populations of Eastern Slovakia: a cross-sectional study. Epidemiol Infect. 2015;143(10):2249-58.

Doğan N, Dinleyici EÇ, Bor Ö, Töz SÖ, Özbel Y. Seroepidemiological survey for Toxocara canis infection in the northwestern part of Turkey. Turkiye Parazitol Derg. 2007;31(4):288-91.

Stensvold CR, Skov J, Møller LN, Jensen PM, Kapel CM, Petersen E, et al. Seroprevalence of human toxocariasis in Denmark. Clin Vaccine Immunol. 2009;16(9):1372-3.

El-Shazly A, Abdel Baset S, Kamal A, Mohammed KA, Sakrs T, Hammad S. Seroprevalence of human toxocariasis (visceral larva migrans). J Egypt Soc Parasitol. 2009;39 (3):731-44.

Poeppl W, Herkner H, Tobudic S, Faas A, Mooseder G, Burgmann H, et al. Exposure to Echinococcus multilocularis, Toxocara canis, and Toxocara cati in Austria: a nationwide cross-sectional seroprevalence study. Vector Borne Zoonotic Dis. 2013;13(11):798-803.

Fallah AA, Pirali-Kheirabadi K, Shirvani F, Saei-Dehkordi SS. Prevalence of parasitic contamination in vegetables used for raw consumption in Shahrekord, Iran: influence of season and washing procedure. Food Control. 2012;25:617-20.

Lötsch F, Obermüller M, Mischlinger J, Mombo-Ngoma G, Groger M, Adegnika AA, et al. Seroprevalence of Toxocara spp. in a rural population in Central African Gabon. Parasitol Int. 2016;65(6):632-634.

Espinoza YA, Huapaya PE, Roldán WH, Jiménez S, Abanto EP, Rojas CA, et al. Seroprevalence of human toxocariasis in Andean communities from the Northeast of Lima, Peru. Rev Inst Med Trop Sao Paulo. 2010;52(1):31-6.

Rubinsky-Elefant G, da Silva-Nunes M, Malafronte RS, Muniz PT, Ferreira MU. Human toxocariasis in rural Brazilian Amazonia: seroprevalence, risk factors, and spatial distribution. Am J Trop Med Hyg. 2008;79(1):93-8.

Loukas A, Hintz M, Linder D, Mullin NP, Parkinson J, Tetteh KK, et al. A family of secreted mucins from the parasitic nematode Toxocara canis bears diverse mucin domains but shares similar flanking six-cysteine repeat motifs. J Biol Chem. 2000:15;275(50):39600-7.

Silva MB, Amor AL, Santos LN, Galvão AA, Oviedo Vera AV, Silva ES, et al. Risk factors for Toxocara spp. seroprevalence and its association with atopy and asthma phenotypes in school-age children in a small town and semi-rural areas of Northeast Brazil. Acta Trop. 2017;174:158-64.

Sharif M, Daryani A, Barzegar G, Nasrolahei M, Khalilian A. Seroprevalence of toxocariasis in schoolchildren in Northern Iran. Pak J Biol Sci. 2010;13(4):180-4.

Nourian A, Amiri M, Ataeian A, Haniloo A, Mosavinasab S, Badali H. Seroepidemiological study for toxocariasis among children in Zanjan-northwest of Iran. Pak J Biol Sci. 2008;11(14):1844-7.

Maraghi S, Rafiei A, Hajihossein R, Sadjjadi S. Seroprevalence of toxocariasis in hypereosinophilic individuals in Ahwaz, south-western Iran. J Helminthol. 2012;86(2):241-4.

Hosseini-Safa A, Mousavi SM, Badorani MBB, Samani MG, Mostafaei S, Darani HY. Seroepidemiology of Toxocariasis in Children (5–15 yr Old) Referred to the Pediatric Clinic of Imam Hossein Hospital, Isfahan, Iran. Iran J Parasitol. 2015;10(4):632-7.

Shahraki MK, Dabirzadeh M, Afshari M, Maroufi Y. Epidemiological Study of Toxocar canis in Children under 14-Years-Old and Dogs in Zabol and Chabahar Districts, Southeast of Iran. Iran J Parasitol. 2017;12(1):101-7.

Alavi SM, Sefidgaran G. Frequency of anti Toxocara antibodies in school children with chronic cough and eosinophilia in Ahwaz, Iran. Pak J Med SCI. 2008;24(3):360-3.