Epidemiological Study of Intestinal Parasites in Referred Individuals to the Medical Centers’ Laboratories of Haji-Abad City, Hormozgan Province, Iran, 2015
Background:intestinal parasitic infection is one of the most prevalent health problems in developing countries. This study was conducted to determine the prevalence of intestinal parasitic infection and its correlation with socio-demographic parameters in Haji-abad, 2015.
MaterialsandMethods:This cross-sectional descriptive study was conducted on 635 samples. After completing questionnaires, stool samples were assessed macroscopically, and microscopically using direct slide smear with saline and lugol, formalin-ether concentration, Ziehl-Neelsen staining to track Cryptosporidium species and Trichrome staining for the samples suspected to amoeba and other indeterminate cases. PCR using specific primers was conducted for Entamoebahistolytica/E. dispar suspected samples. The results were analyzed using SPSSver.16 software.
Results:Of total 635 samples, 198 cases (31.2%) were infected by at least one intestinal parasite. The most common parasites in this area were: Blastocystis sp. (105, 16.5%), Endolimax nana (43, 6.8%), Entamoeba coli (32, 5.0%), Giardia lamblia (31, 4.9%), and Iodamoeba butschlii (11, 1.7%). Enterobius vermicularis (1, 0.2%) was the only detected helminthic infection. Regarding socio-demographic variables, age, residence, sampling month, and job showed a significant correlation with IPIs (p-value=0.031, 0.019, 0.014, 0.012; respectively). None of nine microscopically suspected E. histolytica/E. dispar cases were confirmed by molecular investigations (PCR method) and were considered as E. coli.
Conclusion:In agreement with previous studies, helminthes infections show a dramatic decline compare to protozoa in this study. The relatively high incidence of intestinal protozoan infections in studies performed in Iran supports strategies for preventing the transmission and expansion of these parasites as a priority.
Shokri A, Sharifi S Kh, Hosseini T S, Mahmoodi H. Prevalence of Srongyloides stercularis and other intestinal parasitic infections among mentally retarded residents in central institution of southern Iran. Asian Pacific Journal of Tropical Biomedicine 2012;1(1):88-91.
Sharif M, Daryani A, Asgarian F, Nasrolahei M. Intestinal parasitic infections among intellectual disability children in rehabilitation centers of northern Iran. Res Dev Disabil. 2010;31(4):924-8.
Taheri F, Namakin K, Zarban A, Sharifzadeh G. Intestinal Parasitic Infection among School Children in South Khorasan Province, Iran. J Res Health Sci. 2011;11(1):45-50.
Okyay P, Ertug S, Gultekin B, Onen O Beser E. Intestinal parasites prevalence and related factors in school children, a western city sample -Turkey. BMC publ Hlth. 2004;4:64.
Escobedo AA, Almirall P, Alfonso M, Cimerman S, Rey S, Terry SL. Treatment of intestinal protozoan infections in children. Arch Dis Child. 2009;94:478-82.
Alum A, R. Rubino J , M. Ijaz K. The global war against intestinal parasites—should we use a holistic approach? International Journal of Infectious Diseases. 2010;14:732-8.
Bairami KA , Rezaei S, Babaei Z, Niyyati M, Hashemi SN, Rezaeian M. Enteric Protozoan Parasites in Rural Areas of Bandar-Abbas, Southern Iran: Comparison of Past and Present Situation. Iranian J Publ Health. 2011;40(1):80-5.
Shojaei AA., Alaghehbandan R, Akhlaghi L, Shahi M, Rastegar Lari A. Prevalence of intestinal parasites in a population in south of tehran, Iran. Rev Inst Med trop S Paulo. 2008;50(3):145-9.
Ashtiani MT, Monajemzadeh M, Saghi B, Shams S, Mortazavi SH, Khaki S, et al. Prevalence of intestinal parasites among children referred to Children's Medical Center during 18 years (1991-2008), Tehran, Iran. Ann Trop Med Parasitol. 2011;105(7):507-12.
Niyyati M, Rezaeian M, Zahabion F, Hajarzadeh R, Kia EB. A survey on intestinal parasitic infections in patients referred to a hospital in tehran. Pak J Med Sci. 2009;25(1):87-90.
Daryani A, Sharif M, Nasrolahei M, Khalilian A, Mohammadi A, Barzegar G. Epidemiological survey of the prevalence of intestinal parasites among schoolchildren in Sari, northern Iran. Trans R Soc Trop Med Hyg 2012;106(8):455-9.
Kheirandish F, Tarahi M, Haghighi A, Nazemalhosseini-M E, Kheirandish M. Prevalence of intestinal parasites in bakery workers in khorramabad, lorestan iran. Iran J Parasitol. 2011;6(4):76-83.
Akhlaghi L, Shamseddin J, Meamar AR, Razmjou E, Oormazdi H. Frequency of Intestinal Parasites in Tehran. Iranian J Parasitol. 2009;4(2):44-7.
Mahvi AH, Kia EB. Helminth eggs in raw and treated wastewater in the Islamic Republic of Iran. East Mediterr Health J. 2006;12:137-143.
Meamar AR, Rezaian M, Mohraz M, Zahabiun F, Hadighi R, Kia EB.A comparative analysis of intestinal parasitic infections between HIV+/AIDS patients and non-HIV infected individuals. Iranian J Parasitol. 2007;2:1-6.
Garcia LS, Bruckner DA, Brewer TC, Shimizu RY. Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens. Journal of clinical Microbiol. 1983;18(1):185-90.
Gharavi MJ. Medical Parasitology Laboratory: Teymourzade Publications; 2013. [persian]
Haghighi A, Rezaeian M.The Cultivation of Entamoeba histolitica in Hsr + S Medium. Journal of Kerman University of Medical Sciences. 1998;5(2):60-4.
Zebardast N, Haghighi A, Yeganeh F, Seyyed-Tabaei SJ, Gharavi MJ, Fallahi S, et al. Application of Multiplex PCR for Detection and Differentiation of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii. Iran J Parasitol 2014;9:466-73.
Akhlaghi L, Mafi M, Oormazdi H, Meamar AR, Shirbazou Sh, Tabatabaie F. Frequency of intestinal parasitic infections and related factors among primary school children in Abyek township of Qazvin province (2011 -2012). Annals of Biological Research. 2013;4(8):22-6.
Chattopadhyay AK, Bandyopadhyay S. Seasonal variations of EPG Levels in gastro-intestinal parasitic infection in a southeast asian controlled locale: a statistical analysis. SpringerPlus. 2013;2(205):1-9.
Zebardast N, Gharavi M J, Abadi A R, Seyyed-Tabaei S J, Yeganeh F, Khazan H, et al. Frequency of Intestinal Parasites in Patients With Gastrointestinal Disorders, in Different Parts of Iran During 2012-2013. Int J Enteric Pathog. 2015;3(1):1-5.
Sheiban F, Rezaian M. Study on intestinal protozoa in seven villages of Bandarabass. Iranian J Publ Health. 1981;82(10):1-4.
Kiani H, Haghighi A, Rostami A, Azargashb E, Seyyed Tabaei SJ, Solgi A, et al. Prevalence, risk factors and symptoms associated to intestinal parasite infections among patients with gastrointestinal disorders in nahavand, western Iran. Rev Inst Med Trop Sao Paulo. 2016;58(42):1-7.
Fallahia Sh, Rostami A, Mohammadid M, Ebrahimzadehe F, Pournia Y. Practical parasitology courses and infection with intestinal parasites in students. Journal of Infection and Public Health. 2016;9:654-60.
Badparva E, Kheirandish F, Ebrahimzade F. Prevalence of Intestinal Parasites in Lorestan Province, West of Iran. Asian Pacific J Trop Dis. 2014;4:728-32.
Shahdoust S, Niyyati M, Haghighi A, Azargashb E, Khataminejad MR. Prevalence of intestinal parasites in referred individuals to the medical centers of Tonekabon city, Mazandaran province. Gastroenterol Hepatol BedBench. 2016;9(Suppl. 1):75–9.
Rostami Nejad M, Nazemalhosseini E, Fasihi M, Pourhoseingholi MA, Mowlavi GR, Zali MR. Prevalence of intestinal parasites in patients with gastrointestinal symptoms by focus on soil-transmitted helminthes infection. Gastroenterology and Hepatology from bed to bench. 2010;3(4):190-4.