• Logo
  • SBMUJournals

Inhibitory Effect of Myricetin on Melanoma Cells (A375)

Fereshte Abdolmaleki, Nematollah Gheibi, Hossein Ahmadpour Yazdi
401

Views

PDF

Abstract

Background: Cancer, a major cause of mortality worldwide, is a group of diseases distinguished by uncontrolled growth and expansion of abnormal cells. According to American Cancer Society, melanoma, a kind of skin cancer, is one of the most prevalent cancers. The side effects of chemical treatment developed more demands on natural products. Flavonoids, polyphenol compounds, with anticancer and antioxidant activity attracted more attention to themselves.

Materials and Methods: Through this investigation the effect of myricetin on cell proliferation was determined by MTT (Methylthiazolyl diphenyl-tetrazolium bromide) assay. A375 cell lines were seeded in a 96 wells plate and were exposed to different concentrations of myricetin (10, 15, 20, 40, 60, 80, and 100µΜ). After considered times, the MTT solution was added, then the viability of cells was detected by measuring the absorbance on 570 and 630 nm.

Results: Our finding showed that low concentration of myricetin (up to 25µM) has no toxicity effect. Also the result confirmed the IC50 of myricetin on melanoma cells for three ordered period (24, 48, 72 hours) as following: 50, 40, 35µΜ, respectively.

Conclusion: According to this research, myricetin has anti-proliferative effect on melanoma cells, which can be used as a therapeutic agent. We hope that this study could be used as a mile stone in future researches to acquire confirmative results.


Keywords

Melanoma, Flavonoids, Polyphenol, A375 cell line, Myricetin, Anti-proliferative

References

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA: a cancer journal for clinicians. 2016;66(1):7-30.

American Cancer Society: Cancer Facts and Figures 2016. Atlanta GACS, 2016. Also available onlineExit Disclaimer (PDF - 1.67 MB). Last accessed February 1, 2016.

Ravishankar D, Rajora AK, Greco F, Osborn HM. Flavonoids as prospective compounds for anti-cancer therapy. The international journal of biochemistry & cell biology. 2013;45(12):2821-31.

Pinheiro PF, Justino GC. Structural analysis of flavonoids and related compounds-a review of spectroscopic applications: INTECH Open Access Publisher; 2012.

Navarro Hoyos M, Sánchez-Patán F, Murillo Masis R, et al. Phenolic Assesment of Uncaria tomentosa L.(Cat’s Claw): Leaves, Stem, Bark and Wood Extracts. Molecules. 2015;20(12):22703-17.

Kanakis C, Nafisi S, Rajabi M, et al. Structural analysis of DNA and RNA interactions with antioxidant flavonoids. Journal of Spectroscopy. 2009;23(1):29-43.

Büchter C, Ackermann D, Havermann S, et al. Myricetin-mediated lifespan extension in Caenorhabditis elegans is modulated by DAF-16. International journal of molecular sciences. 2013;14(6):11895-11914.

Feng J, Chen X, Wang Y, et al. Myricetin inhibits proliferation and induces apoptosis and cell cycle arrest in gastric cancer cells. Molecular and cellular biochemistry. 2015;408(1-2):163-70.

Lee DH, Lee CS. Flavonoid myricetin inhibits TNF-α-stimulated production of inflammatory mediators by suppressing the Akt, mTOR and NF-κB pathways in human keratinocytes. European Journal of Pharmacology. 2016;784:164-72.

Pan H, Hu Q, Wang J, et al. Myricetin is a novel inhibitor of human inosine 5′-monophosphate dehydrogenase with anti-leukemia activity. Biochemical and Biophysical Research Communications. 2016;477(4):915-22.

Mu M, An P, Wu Q, et al. The dietary flavonoid myricetin regulates iron homeostasis by suppressing hepcidin expression. The Journal of nutritional biochemistry. 2016;30:53-61.

Erdmann F, Lortet‐Tieulent J, Schüz J, et al. International trends in the incidence of malignant melanoma 1953–2008—are recent generations at higher or lower risk? International Journal of Cancer. 2013;132(2):385-400.

Ren W, Qiao Z, Wang H, Zhu L, Zhang L. Flavonoids: promising anticancer agents. Medicinal research reviews. 2003;23(4):519-34.

Csokay B, Prajda N, Weber G, Olah E. Molecular mechanisms in the antiproliferative action of quercetin. Life Sciences. 1997;60(24):2157-63.

Middleton Jr E, Kandaswami C. The impact of plant flavonoids on mammalian biology: implications for immunity, inflammation and cancer. The flavonoids. London: Chapman and Hall. 1994.

Ferriola PC, Cody V, Middleton E. Protein kinase C inhibition by plant flavonoids: kinetic mechanisms and structure-activity relationships. Biochemical pharmacology. 1989;38(10):1617-24.

Geahlen RL, Koonchanok NM, McLaughlin JL, Pratt DE. Inhibition of Proteiin-Tyrosine Kinase Activity by Flavanoids and Related Compounds. Journal of natural products. 1989;52(5):982-6.

Wang I-K, Lin-Shiau S-Y, Lin J-K. Induction of apoptosis by apigenin and related flavonoids through cytochrome c release and activation of caspase-9 and caspase-3 in leukaemia HL-60 cells. European Journal of Cancer. 1999;35(10):1517-25.

Wei Y-q, Zhao X, Kariya Y, Fukata H, Teshigawara K, Uchida A. Induction of apoptosis by quercetin: involvement of heat shock protein. Cancer Research. 1994;54(18):4952-7.

Galati G, O'Brien PJ. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radical Biology and Medicine. 2004;37(3):287-303.

Kuntz S, Wenzel U, Daniel H. Comparative analysis of the effects of flavonoids on proliferation, cytotoxicity, and apoptosis in human colon cancer cell lines. European journal of nutrition. 1999;38(3):133-42.

Carli CBdA, Matos DCd, Lopes F, et al. Isolated flavonoids against mammary tumour cells LM2. Zeitschrift für Naturforschung C. 2009;64(1-2):32-6.

Kumamoto T, Fujii M, Hou D-X. Myricetin directly targets JAK1 to inhibit cell transformation. Cancer letters. 2009;275(1):17-26.




DOI: https://doi.org/10.22037/nbm.v5i3.13535