Effect of Olive Leaf Extract on Cytokines Secreted by Macrophage
Background: Herbal medicines in compared with chemical drugs have fewer side effects and can be a good medicinal alternative. The olive includes 20 different species of the family Oleaceae, with Olea europaea as the most recognized. Several studies have shown the immunomodulating effects of olive leaf extract. This study aimed to identify the immunoregulatory effect of olive leaf Sevillana variety on interleukins 12 and 10 which resulted from the murine macrophages in vitro.
Materials and Methods: In order to isolate macrophages, peritoneal macrophages BALB/C were used. To determine the cytotoxic effect of different concentrations of the olive leaf extract on macrophages, MTT assay was performed. Concentrations of 200, 100, 50, 25, 12.5, 6.25, and 3.1μg/ml in the time intervals of 12, 24, and 48 hours were evaluated. Three appropriate concentrations were selected to commence with the study of the determination of the amount of cytokines. Cell culture supernatant growth medium supernatant was collected at 12, 24, and 48 hours after adding the extract in order to examine the amount of cytokines. ELISA test was conducted using interleukins 12 and 10 measurement kits.
Results: CC50 of the olive leaf extract at 12, 24, and 48 hours was 260.3, 170.5, and 150 μg/ml, respectively. According to the results, an increase in the concentration and duration of the study resulted in observable significant differences in the production of interleukins 10 and 12. As a result, the production of IL-10 and 12 experienced decreases and increases, respectively.
Conclusion: It seemed probable that the olive leaf extract had the capability to increase the production of IL-12 through activation of the classic macrophages and also deactivate the regulatory macrophages with an increase in IL-12 and a decrease in IL-10. Therefore, this can strengthen the immune system of the host in the early stages of infection. The other immunomodulatory effects of olive leaf must be considered by appropriate research.
Joannis D. Kyriazisa, Nektarios Aligiannisb, Panagiotis Polychronopoulosb. Leishmanicidal activity assessment of olive tree extracts. J Phytomedicine. 2013;20:275-81.
Manna C, Angelo SD, Migliardi V, Loffredi E, Mazzoni O, Morrica P, et al. Protective effect of phenolic fraction from virgin olive oils against oxidative stress in human cells. J Agric Food Chem 2002;50:6521-6.
Owen RW, Giacosa A, Hull WE, Haubner R, Spiegelhalder B, Bartsch H. The antioxidant/anticancer potential of phenolic compounds isolated from olive oil. Eur J Cancer. 2000;36:1235-47.
Bisingnano G, Tomaino A, Lo Cascio R, Crisafi G, Uccella N, Saija A. On the in vitro antimicrobial activity of oleuropein and hydroxtyrosol. J Pharm Pharmacol. 1999;51:971-4.
Lo Scalzo R, Scarpati ML, Verzengnassi B,Vita G. Olea europaea chemical repellent to Dacus oleae females. J Chem Ecol. 1994;20:1813-23.
Andrews P, Busch JLHC, Joode TD,Groenewegen A, Alexandre H. Sensory properties of virgin olive oil polyphenols: identification of deacetoxy-ligstroside aglycon as a key contributor to pungency. J Agric Food Chem. 2003;51:1415-20.
Hashemi P, Delfan B, Ghiasvand A. The study of Oleuropein in the leaves of olive trees grown in the city of Khorramabad. J Lorestan university of Medical Sciences. 2008;4(10):99-106.
Eryuce N. Seasonal variation of nutrient contents in the leaves of an oil-olive variety from the Ayvalik district. Ege Universitesi Ziraat Fakultesi Dergisi. 1980;17(2):209-21.
Lust J, Martin JB. The Herb Book:The Most Complete Catalog of Herbs Ever Published. 2th ed. New York: Courier Corporation. 2014.
Scure LS, Simioni PU, Grabriel DL, Saviani EE, Modol LV, Tamashiro WM, et al. Suppression of nitric oxide production in mouse macrophage by soybean flavonoides acctumulated in response to nitroprusside and fungal elicitation. J BMC Biochem. 2004;21(2):505-50.
Chen LC, Pace JL, Russell SW, Morrison DC. Altered regulation of inducible nitric oxide synthase expression in macrophages from senescent mice. J Infect Immun. 1996;64(10):4288-98.
Dutta A, Bandyopadhyay S, Mandal C, Chatterjee M. Development of a modified MTT Sassay for screening antimonial resistant field isolates of Indian visceral eishmaniasis. J Parasitology international. 2005;54(2):119-22.
Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival:odifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of immunological methods. 1986;89(2):271-7.
Kheirandish F,Tarahi MJ, Ezatpour B. Prevalence of intestinal parasites among food handlers in West of Iran. Rev Inst Med Trop Sao Paulo. 2014;56:111-4.
Badparva E, Kheirandish F, Ebrahimzade F. Prevalence of intestinal parasites in Lorestan Province, West of Iran. Asian Pac J Trop Dis. 2014;4:930- 4.
Kheirandish F, Tarahi MJ, Haghighi A, Nazemalhosseini- Mojarad E, Kheirandish M. Prevalence of intestinal parasites in bakery workers in Khorramabad, Lorestan Iran. Iran J Parasitol. 2011;6:76-83.
- Kheirandish F, Badparva E, Haghighi A, Nazemalhosseini Mojarad E, Kazemi B. Differential diagnosis of Entamoeba spp. in gastrointestinal disorder patients in Khorramabad, Iran. Afr J Microbiol Res. 2011;5(18):2863-6.
Kheirandish F, Chegeni Sharafi A, Kazemi B, Mohebali M, Sarlak A, Tarahi MJ, Holakouee K, Hajaran H. Identification of Leishmania species using PCR assay on giemsa-stained slides prepared from cutaneous leishmaniasis patients. Iran J Parasitol. 2013;8(3):382-8.
Kheirandish F, Chegeni Sharafi A, Kazemi B, Bandehpour M, Tarahi MJ, Khamesipour A. First molecular identification of Leishmania species in a new endemic area of cutaneous leishmaniasis in Lorestan, Iran. Asian Pac J Trop Med. 2013;6(9):713-7.
Croft SL, Sundar S, Fairlamb AH. Drug resistance in leishmaniasis. Clin Microb Rev. 2006;19:11–26.
Kheirandish F, Delfan B, Farhadi S, Ezatpour B, Khamesipour A, Kazemi B, et al. The effect of Satureja khuzestanica essential oil on the lesions induced by Leishmania major in BALB/c mice. African Journal of Pharmacy and Pharmacology. 2011;5(5):648-53.
Mahmoudvand H, Saedi Dezaki E, Kheirandish F, Ezatpour B, Jahanbakhsh S, Fasihi Harandi M. Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts. Korean J Parasitol. 2014;52(6):1-7.
Uccella N. Olive biophenols: biomolecular characterization, distribution and phytolexin histochemical localization in the drupes. J Trends Food Sciences. 2001;11:315-27.
Rosiqnoil P, Fuccelli R, Fabiani R, Servili M, Morozzi G. Effect of olive phenols on the production of inflammatory mediators in freshly isolated human monocytes. J of Nutritional Biochemistry. 2013;24:1513-9.
Lee-Huanq S, Zhanq l, Huanq PL, Chanq YT, Huanq PL. Anti- HIV activity of olive leaf extract (OLE) and modulation of host cell gene expression by HIV-1 infection and OLE treatment. J Biochem Biophys Res Commun. 2003;307(4):1029-37.
Gharavi MJ. Textbook of clinical protozoology.3th ed. Tehran: Tabib Publication. 1378. (Text in Persian).
Edrisian Gh, Rezaeian M, Ghorbani M, Keshavarz H, Mohebali M. In: Mohebali M editor. Medical Protozoology. 1ed. Tehran: Medical university Press; 1386.(Text in Persian)
Karimi A, Jahromi H, Hadipour Jahromi M. In: Jahromi H editors. Viseral Leishmania in Iran & World.1th ed. Tehran:Health & Nutrition Cultural Institute; 2008.( Text in Persian).