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CRISPR-Cas9 Mediated Capsule Gene Silencing in Escherichia coli

Mojgan Bandehpour, Tina Nafarieh, Fatemeh Zahedipour, Afsaneh Tavasoli, Bahram Kazemi
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Abstract

Background: Genomic engineering of Escherichia coli is applied to design and produce recombinant proteins as the new drugs. The aim of this study was to CRISPR-Cas9 mediated capsule gene silencing in E. coli.

Materials and Methods: We suppressed genes involved in capsule expression of E.coli by CRISPR cas9 process. The constructed E.coli was confirmed by microscopic smear, transmission electron microscopy and T7 phage influence assay.

Results: The results were shown that the inhibition of capsule production was carried out successfully and there was not any capsule layer around the bacteria.

Conclusion: E. coli without any capsule around may proper for replacement of it with other molecules in future.


Keywords

capsule, CEISPR, Escherichia coli, TEM

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DOI: https://doi.org/10.22037/nbm.v6i4.21672