Comparative effects of Nucleostemin silencing in human Molt-4 and Jurkat leukemia T-ALL cells

Marveh Rahmati, Mohammad Amin Moosavi, Seyedmehdi Nourashrafeddin, Zoya Hojabri, Akbar Hasani, Nosratollah Zarghami

Abstract


228

     Nucleostemin (NS), a stem cell-abundant nucleolar protein, is critical for maintaining the self-renewal and proliferative properties of normal and cancerous stem cells. Recent data suggests that NS signaling is important for proliferation of T-cells and leukemia cells. This study was conducted to verify the role of NS in pathogenesis and treatment of T-cell acute lymphocytic leukemia (T-ALL). Our results revealed that RNA interference-mediated NS silencing primarily affected clonogenicproperty of T-ALL cells by limiting their self-renewal potential in vitro.These effects were accompanied with inhibition of proliferation and early apoptosis in Jurkat cells (p53-null) while late apoptosis in Molt-4 (p53 functional) T-ALL cells. Collectively, our results suggest that NS is a critical regulator in self-renewal and apoptosis of differentT-ALL cells. This suggests therapeutic potential of this gene in leukemia.


Keywords


Acute lymphoblastic leukemia; Apoptosis; Nucleostemin; Gene silencing

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DOI: https://doi.org/10.22037/jps.v6i3.9787

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"Journal of Paramdedical Sciences", is a publication of "School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences" and "Iranian Society of Medical Proteomics".

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EISSN: 2008-4978

PISSN: 2008-496X