Advances in proteomics analytical techniques

Reyhaneh Farrokhi Yekta, Mehdi Koushki, Nasrin Amiri DashAtan

Abstract


265

   Proteins are fundamental components of cells which mediate many essential biological processes. Proteomics is a rapidly growing field for the study of proteome, the protein complement expressed by the genome of an organism or cell type. The large-scale analysis of proteins leads to a more comprehensive view of molecular and cellular pathways that improves the overall understanding of the complex processes supporting the living systems. The analysis of proteome is significantly challenging due to high dynamic range and difficulties in assessment of low abundance proteins and the absence of efficient purification and identification techniques. A variety of methods have been utilized for protein studies including gel-based techniques, protein microarrays, mass spectrometry-based approaches such as MALDI and SELDI, high and ultra-performance liquid chromatography and fourier transform ion cyclotron resonance mass spectrometry. NMR spectroscopy and X-Ray crystallography methods are also used for structural study of proteins. This review aims to give a brief overview of some of the above techniques and their most recent advances. We also introduce Proteominer, a recent protein enrichment technology for the exploration of the entire proteome content

Keywords


Mass Spectrometry; Electrophoresis; Proteomics Techniques.

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DOI: https://doi.org/10.22037/jps.v6i3.9773

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"Journal of Paramdedical Sciences", is a publication of "School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences" and "Iranian Society of Medical Proteomics".

"Journal of Paramdedical Sciences" is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

EISSN: 2008-4978

PISSN: 2008-496X