Lentiviral vectors titration using real-time PCR

Amir Allahverdi, Fatemeh Eskandari, Mohammad Hossein Moghadasi, Mehdi Azad, Mehdi Goudarzi, Saied Abroun, Masoud Soleimani

Abstract


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Lentiviral vectors (LVs) are useful vehicle for genetransfer to dividing and non-dividing cells and genetic manipulations. However, the use of lentiviruses in studies requires an accurate titration technique.Quantitative real-time PCR (qPCR) is a sensitive technique for the indication and quantitation of retrovirals particles. In this study, we used the qPCR for lentiviral vector titeration. The puromycin resistance gene as templates for an SYBR green-based real-time qPCR method and detect lentiviral copy number integrated lentiviral DNA. Consequently, this studyshowed that theusing ofantibioticresistance genesviral particles titration maybeefficient with highly accuracy.

 


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DOI: https://doi.org/10.22037/jps.v6i1.8324

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"Journal of Paramdedical Sciences", is a publication of "School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences" and "Iranian Society of Medical Proteomics".

"Journal of Paramdedical Sciences" is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

EISSN: 2008-4978

PISSN: 2008-496X