Detection of Human papillomavirus 16 and 18 types in oral squamous cell carcinoma patients in Yazd, Iran: A Case-Control Study

Seyyed Hossein Tabatabai, Minoo Nabieyan, Mohammad Hasan Sheikhha, Shokouh Taghi Poor zahir, Sedigheh Zarmehi, Azadeh Andisheh Tadbir, Nayeb Ali Ahmadi




        The aim of this study was to ascertain the prevalence of human papillomavirus (HPV) 16/18 in oral squamous cell carcinoma (OSCC) and normal oral mucosa in Iranian individuals with OSCC and their association with various risk factors. The investigation followed a hospital-based case-control design. Cases were individuals with diagnosed OSCC, age- and sex-matched with controls. Sixty-six paraffin embedded tissue samples (39 OSCC and 27normal oral mucosa) were studied. DNA was purified to amplify HPV-DNA using HPV type specific primers-mediated polymerase chain reaction (PCR) method.Mean age was 64.2 ±14 years in cases (range 25-81 years), and 63.5 ±13.5 years in controls (range 22-78 years). Forty-three percent (17/39) of OSCC but none of normal oral mucosa were positive for HPV 16/18 DNA (p = 0.001). The most frequent type in patient group was HPV-16 type. The prevalence rate was high older in age (>50 years) and gender (male) and tongue tissue but no significant association was found (p=0.7, p=0.5 and p=0.42 respectively). We found a significant association of HPV16/18 with oral squamous cell carcinoma. Also, HPV16/18 is a co-factor in oral carcinogenesis. In addition, also it is suggested that HPV is as a risk factor independent of alcohol and tobacco can be effective in creating OSCC.



human papillomavirus 16 and 18 types; oral squamous cell carcinoma; PCR

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"Journal of Paramdedical Sciences", is a publication of "School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences" and "Iranian Society of Medical Proteomics".

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EISSN: 2008-4978

PISSN: 2008-496X