Human βhsp90 as adjuvant in HCV Recombinant vaccine

Zarin Sharifnia, Nariman Mosaffa, Mahvash khodabandeh, Roya Yaraee, Bahram Kazemi, Mojgan Bandehpour

Abstract


135

There are more than 350 million individuals with hepatitis C in the world. One of the important problems in vaccine project is development of effective and suitable adjuvant in human vaccines. At present research we applied human βHsp90 protein as an adjuvant in recombinant HCV vaccine design. The thermal vector of pGP1-2 was used for human heat shock protein 90 expression. This protein injected to BalbC mice as an adjuvant together with recombinant protein of HCV core. The combination of these proteins was used and we evaluated the humoral and cellular immunity and the cytokine secretion of inguinal and popliteal lymph nodes lymphocytes were analyzed in vitro and ex vivo conditions. So the combination of Core protein together with hsp90 induced total IgG and IgG2a secretion. The spleen lymphocytes proliferation were increased equal to serum IgG2a level that was constant in second time bleeding with significant different to complexes with freund’s adjuvant. At first IL-4 and IL-5 cytokines were increased, after one week it decreased. Production of IL-4 showed there was no hypersensitivity reaction after vaccine injection.

Keywords


Hsp90; core; lymphocyte; IgG; pETDuet-1 vector

Full Text:

PDF

100



DOI: https://doi.org/10.22037/jps.v4i3.4667

Refbacks

  • There are currently no refbacks.


"Journal of Paramdedical Sciences", is a publication of "School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences" and "Iranian Society of Medical Proteomics".

"Journal of Paramdedical Sciences" is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

EISSN: 2008-4978

PISSN: 2008-496X