• Logo
  • SBMUJournals

Low-Level Laser Action on Orthodontically Induced Root Resorption: Histological and Histomorphometric Evaluation

Eliziane Cossetin Vasconcelos, Jose Fernando Castanha Henriques, Marinês Vieira Silva Sousa, Rodrigo Cardoso de Oliveira, Alberto Consolaro, Arnaldo Pinzan, Fernanda Pinelli Henriques, Caroline Nemetz Bronfman
688

Views

PDF

Abstract

Introduction: Studies have been conducted to develop a means of preventing, controlling or reducing orthodontically induced root resorption. Phototherapy has demonstrated effectiveness as an anti-inlammatory and, considering the inlammatory origin of this pathology, this study evaluated the effects of laser on root resorption.

Methods: The research was conducted among 54 80-day-old male Wistar rats, with weights of 280 ± 40 g. Phototherapy consisted of a diode laser (Ga-Al-As), calibrated with a wavelength of 808 nm, an output power of 100 mW, 2.1 J or 96 J of energy and area of 0.0028 cm2. The application was continuous, punctual and with contact. The left irst maxillary molar was moved by a super-elastic closed spring with a pre-calibrated and constant force of 25 g. The specimens were irradiated every 48 hours, totaling three or six times, depending on the group to which they belonged. Euthanasia was made in the 7th or 10th day after the onset of movement. The histological and histomorphometric examination was performed with sections of 6 μm stained with hematoxylin and eosin (H&E).

Results: Considering the dosimetry studied, when compared the subgroups with the same time of movement, 7 or 10 days, the low-level laser (LLL) has no statistically signiicant effect on the root resorption. As expected, differences were found between groups with different time of movement.

Conclusion: Based on the result, this dosimetry does not seem to be clinically recommended to avoid or reduce inlammatory root resorption, but it also does not induce any root surface alteration.


Keywords

Resorption, Root, Laser Therapy, Tooth Movement, Phototherapy

References

Lindskog S, Blomlof L, Hammarstrom L. Comparative effects of parathyroid hormone on osteoblasts and cementoblasts. J Clin Periodontol. 1987;14(7):386-389. doi:10.1111/j.1600-051x.1987.tb01541.x.

Brezniak N, Wasserstein A. Orthodontically induced inflammatory root resorption. Part I: The basic science aspects. Angle Orthod. 2002;72(2):175-179.

Brudvik P, Rygh P. The initial phase of orthodontic root resorption incident to local compression of the periodontal ligament. Eur J Orthod. 1993;15(4):249-263. doi:10.1093/ ejo/15.4.249.

Mohandesan H, Ravanmehr H, Valaei N. A radiographic analysis of external apical root resorption of maxillary incisors during active orthodontic treatment. Eur J Orthod. 2007;29(2):134-139. doi:10.1093/ejo/cjl090.

Gonzales C, Hotokezaka H, Karadeniz EI, et al. Effects of fluoride intake on orthodontic tooth movement and orthodontically induced root resorption. Am J Orthod Dentofacial Orthop. 2011;139(2):196-205. doi:10.1016/j. ajodo.2009.05.029.

Gonzales C, Hotokezaka H, Matsuo K, et al. Effects of steroidal and nonsteroidal drugs on tooth movement and root resorption in the rat molar. Angle Orthod. 2009;79(4):715-726. doi:10.2319/072108-381.1.

Sato M, Grasser W, Endo N, et al. Bisphosphonate action. Alendronate localization in rat bone and effects on osteoclast ultrastructure. J Clin Invest. 1991;88(6):2095- 2105. doi:10.1172/jci115539.

Sirisoontorn I, Hotokezaka H, Hashimoto M, et al. Orthodontic tooth movement and root resorption in ovariectomized rats treated by systemic administration of zoledronic acid. Am J Orthod Dentofacial Orthop. 2012;141(5):563-573. doi:10.1016/j.ajodo.2011.11.016.

Verna C, Hartig LE, Kalia S, Melsen B. Influence of steroid drugs on orthodontically induced root resorption. Orthod Craniofac Res. 2006;9(1):57-62. doi:10.1111/j.1601- 6343.2006.00342.x.

Albertini R, Villaverde AB, Aimbire F, et al. Anti- inflammatory effects of low-level laser therapy (LLLT) with two different red wavelengths (660 nm and 684 nm) in carrageenan-induced rat paw edema. J Photochem Photobiol B. 2007;89(1):50-55. doi:10.1016/j. jphotobiol.2007.08.005.

Casalechi HL, Leal-Junior EC, Xavier M, et al. Low-level laser therapy in experimental model of collagenase-induced tendinitis in rats: effects in acute and chronic inflammatory phases. Lasers Med Sci. 2013;28(3):989-995.

Pires D, Xavier M, Araujo T, Silva JA Jr, Aimbire F, Albertini

R. Low-level laser therapy (LLLT; 780 nm) acts differently on mRNA expression of anti- and pro-inflammatory mediators in an experimental model of collagenase- induced tendinitis in rat. Lasers Med Sci. 2011;26(1):85-94. doi:10.1007/s10103-010-0811-z.

Carvalho ED, Costa FT, Campos MS, et al. Root surface treatment using diode laser in delayed tooth replantation: radiographic and histomorphometric analyzes in rats. Dent Traumatol. 2012;28(6):429-436. doi:10.1111/j.1600- 9657.2011.01108.x.

Saito CT, Gulinelli JL, Panzarini SR, et al. Effect of low-level laser therapy on the healing process after tooth replantation: a histomorphometrical and immunohistochemical analysis. Dent Traumatol. 2011;27(1):30-39. doi:10.1111/ j.1600-9657.2010.00946.x.

Fonseca PD, de Lima FM, Higashi DT, et al. Effects of light emitting diode (LED) therapy at 940 nm on inflammatory root resorption in rats. Lasers Med Sci. 2013;28(1):49-55. doi:10.1007/s10103-012-1061-z.

Heller IJ, Nanda R. Effect of metabolic alteration of periodontal fibers on orthodontic tooth movement. An experimental study. Am J Orthod. 1979;75(3):239-258. doi:10.1016/0002-9416(79)90272-0.

de Oliveira RC, de Oliveira FH, Cestari TM, Taga R, Granjeiro JM. Morphometric evaluation of the repair of critical-size defects using demineralized bovine bone and autogenous bone grafts in rat calvaria. Clin Oral Implants Res. 2008;19(8):749-754.

Fracalossi AC, Santamaria MJ, Consolaro MF, Consolaro

A. [Experimental tooth movement in murines: study period and direction of microscopic sections]. R Dental Press Ortodon Ortop Facial. 2009;14(1):143-157.

Schwarz AM. Tissue changes incident to orthodontic tooth movement. Int J Orthod. 1932;18:331-352.

Storey E. The nature of tooth movement. Am J Orthod. 1973;63(3):292-314. doi:10.1016/0002-9416(73)90353-9.

Smith KC. Laser (and LED) therapy is phototherapy. Photomed Laser Surg. 2005;23(1):78-80. doi:10.1089/ pho.2005.23.78.

Lobko VV, Karu TI, Letokhov VS. [Is the coherence of low-intensity laser light essential for its effect on biological objects?]. Biofizika. 1985;30(2):366-371.

Karu T. Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B. 1999;49(1):1-17.

Shimizu N, Yamaguchi M, Goseki T, et al. Inhibition of prostaglandin E2 and interleukin 1-beta production by low- power laser irradiation in stretched human periodontal ligament cells. J Dent Res. 1995;74(7):1382-1388.

Andreasen JO. External root resorption: its implication in dental traumatology, paedodontics, periodontics, orthodontics and endodontics. Int Endod J. 1985;18(2):109- 118.

Andreasen JO. Review of root resorption systems and models: etiology of root resorption and the homeostatic mechanisms of the periodontal ligament. In: Davidovitch Z, ed. The biological mechanisms of tooth eruption and root resorption. Birmingham, Alabama: EBSCO Media; 1988:9-21.

Brudvik P, Rygh P. Multi-nucleated cells remove the main hyalinized tissue and start resorption of adjacent root surfaces. Eur J Orthod. 1994;16(4):265-273.

Altan BA, Sokucu O, Ozkut MM, Inan S. Metrical and histological investigation of the effects of low-level laser therapy on orthodontic tooth movement. Lasers Med Sci. 2012;27(1):131-140. doi:10.1007/s10103-010-0853-2.

Kawasaki K, Shimizu N. Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats. Lasers Surg Med. 2000;26(3):282-291.

Brudvik P, Rygh P. Transition and determinants of orthodontic root resorption-repair sequence. Eur J Orthod. 1995;17(3):177-188. doi:10.1093/ejo/17.3.177.

Vinck EM, Cagnie BJ, Cornelissen MJ, Declercq HA, Cambier DC. Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation. Lasers Med Sci. 2003;18(2):95-99. doi:10.1089/pho.2009.2732.

Habib FA, Gama SK, Ramalho LM, et al. Laser-induced alveolar bone changes during orthodontic movement: a histological study on rodents. Photomed Laser Surg. 2010;28(6):823-830. doi:10.1089/pho.2009.2732.

Brudvik P, Rygh P. The repair of orthodontic root resorption: an ultrastructural study. Eur J Orthod. 1995;17(3):189-198. doi:10.1093/ejo/17.3.189.




DOI: https://doi.org/10.22037/jlms.v7i3.8748