• Logo
  • SBMUJournals

The Effect of Low-Level Laser Therapy on Human Leukemic Cells

Somayeh Dastanpour, Jalil Momen Beitollahi, Kazem Saber
308

Views

PDF

Abstract

Introduction: Laser phototherapy is used for the treatment of chemotherapy-induced oral mucositis in patients with leukemia, although there are limited data supporting the safety of this method. This study aimed to evaluate the effect of different doses of low-level laser on proliferation of acute myeloid leukemia (AML) cell line (KG-1a) in vitro.

Methods: A plastic flask containing 5,000,000 KG-1a cultured cells was provided by Iran Pasteur Institute. KG-1a cell line has been produced from the bone marrow aspirate of a 59-year-old white male with acute myelogenous leukemia. Upon completion of the proliferation steps of KG-1a cell line, 7×104 cells were placed in 96-well tissue culture plates. All the surrounding wells were filled with Wright-Giemsa stain in order to prevent laser from scattering to the neighboring wells. In total, 28 plates were prepared using this method. After a forty-eight hours incubation period, irradiation was performed in continuous mode with an infrared laser of 810nm wavelength. After 24 hours, cells cultures were exposed to one, two, or three applications of laser irradiation. Irradiation exposures were performed at energy densities of 5, 10, and 20 J/cm2. Each experiment included 18 replicates for each application of laser and 6 replicates of negative/untreated controls. For experiments with two and three repeated exposures, the irradiation applications were separated by 48 hours. All the culture plates were incubated for seven days. Cell proliferation was evaluated using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)  assay after seven days. Spectroscopy (620nm) was used to determine the optical density (OD) of both irradiated and control samples.

Results: Significant increase in cell proliferation was seen only after two exposures at energy density of 20J/cm2 (P=0.021).

Conclusion: Although LLLT is commonly used to treat radiotherapy- or chemotherapy- induced mucositis, as long as further studies demonstrate that different wavelengths and doses of laser phototherapy are safe and effective in treatment of mucositis, clinicians should remain cautious regarding the use of this treatment modality to treat patients with malignancies.


Keywords

low-level laser therapy; leukemia; cell culture.

References

Kreisler M, Christoffers AB, Willershausen B, D’Hoedt B. Low – level 809 nm GaALAs laser irradiation increases the proliferation rate of human laryngeal carcinoma cells in vitro. Lasers Med Sci. 2003; 18: 100 – 3.

Pislea M, Seremet T, Katona G, Mucanu M, Doaga JO, Radu E et al. Low level long wave length laser irradiation effects on cell cycle progression and apoptosis of energy restricted jurkat T-cells. Romanian J Biophys. 2009; 19: 1- 18.

Gulsoy M, Ozer GH, Bozkulak O, Tabakoglu HO, Aktas E, Deniz G. The biological effects of 632.8nm low energy He-Ne laser on peripheral blood mononuclear cells in vitro. J Photochem Photobiol B. 2006; 82: 199 – 202.

Renno ACM, McDonnell PA, Parizotto NA, Laakso E-L. The effects of laser irradiation on osteoblasts and osteosarcoma cells proliferation and differentiation in vitro. Photomed Laser Surg. 2007; 25: 275 – 80.

Pinheiro ALB, Nascimento SC, Vieira ALDB, Rolim AB, Silva PSD, Brugnera JrA. Does LLLT stimulate laryngeal carcinoma cells? An in vitro study. Braz Dent. 2002; 13: 109 – 12.

Frigo L, Luppi JSS, Favero GM, Maria DA, Penna SC, Bjordal JM et al. The effect of low- level laser irradiation (In-Ga-As-660nm) on melanoma in vitro and in vivo. BMC Cancer. 2009; 9: 404 – 11.

Schaffer M, Sorka R, Fuchs C, Schrader-Richardt U, Schaffer PM, Duhmke E. Biomodulative effects induced by 805nm laser light irradiation of normal and tumor cells. J Photomed Photobiol B. 1997; 40: 253 – 7.

Nes AG. Patients with moderate chemotherapy–induced mucositis: pain therapy using low intensity lasers. Int Nurs Rev. 2005; 52: 68 – 72.

Cruz LB, Ribeiro AS, Rech A, Rosa LGN, Castro JRCG, Brunetto AL. Influence of low–energy laser in the prevention of oral mucositis in children with cancer receiving chemotherapy. Pediatr Blood Cancer. 2007; 48: 435 – 40.

Schubert MM, Eduardo FP, Guthrie KA, Franquin JC, Bensadoun RJ, Migliorati CA. A Phase III randomized double–blind placebo–controlled clinical trial to determine the efficacy of low level laser therapy for prevention of oral mucositis in patients undergoing hematopoietic cell transplantation. Support Care Cancer. 2007; 15: 1145- 54.

Sylvester PW. Methods in Molecular Biology, Volume 716: Drug Design and Discovery: Methods and Protocols. In: Satyanarayanajois SD (ed) Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability, 1st ed. Humana Press; 2011. 157-68.

Tunner J, Hode L (Eds). Laser therapy: clinical practice and scientific background. In: Biostimulation. Grängesberg: Prima books; 2008. 61-115.

Powell K, Low P, McDonnell A, Laakso EL, Ralph SJ. The effect of laser irradiation on proliferation of human breast carcinoma, melanoma, and immortalized mammary epithelial cells. Photomed Laser Surg. 2010; 28: 115- 23.

Werneck CE, Pinheiro ALB, Pacheco MTT, Soares CP, De Castro JLF. Laser light is capable of inducing proliferation of carcinoma cells in culture: A spectroscopic in vitro study. Photomed Laser Surg. 2005; 23: 300 – 3.

Buylin VA (Eds). Low–intensity laser therapy of various diseases. Sao Paolo: CRC Press; 2001.

De Castro JLF, Pinheiro ALB, Wereneck CE, Soares CP The effects of laser therapy on proliferation of oral KB carcinoma cells: an in vitro study. Photomed Laser Surg. 2005; 23: 586 – 9.

Pinheiro ALB, Nascimento SC, Vieira ALDB, Rolim AB, Silva PSD, Brugneva JrA. Effects of low–level laser therapy on malignant cells: in vitro study. J Clin Laser Med Surg. 2002; 20: 23 – 6.

Friedmann H, Lubart R. Towards an explanation of visible and infrared laser induced stimulation and damage of cell cultures. Laser Ther. 1992; 3: 39–42




DOI: https://doi.org/10.22037/jlms.v6i2.7930