• Logo
  • SBMUJournals

Antimicrobial Activity of Cold Plasma Treatment on Acrylic Denture Bases: An In Vitro Evaluation

Mohammad Asnaashari, Salar Motamedi, Negin Asnaashari, Saranaz Azari-Marhabi




Introduction: Cross-contamination between dental office and prosthetic laboratories is of utmost importance. The dental prosthesis could harbor tones of microorganisms harmful to health staff. It has been estimated that more than 60% of the prostheses delivered to clinics from laboratories or vice versa are contaminated with pathogenic microorganisms. Several disinfection methods and chemicals have been proposed for sanitizing prostheses. Cold plasma, or nonthermal plasma, has been introduced as a new method in medical equipment disinfection. The current study aimed to compare the antimicrobial effect of cold plasma and the routinely used alcohol-based impression disinfectant ASEPTOPRINT® on disinfecting dentures.

Methods: In this experimental study, 30 maxillary complete dentures were used as the microbial source. Dentures were randomly divided into 3 groups: (1) cold plasma treatment for 30 seconds, (2) cold plasma for 60 seconds, (3) ASEPTOPRINT® spray. Microbial culturing was taken before and after disinfection in each group on both Blood Agar plates and Soborou Dextrose Agar (SDA) plates. After 48 hours incubation at 37°C, the number of colonies was counted using a digital colony counter and differences between the groups were analyzed.

Results: A significant reduction in microbial colonies was observed after using all disinfection methods. ASEPTOPRINT® spray showed more antimicrobial effects comparing to cold plasma.

Conclusion: The antimicrobial effect of ASEPTOPRINT® spray solution is more than cold plasma application on both microbial and fungal microorganisms. The time of plasma application was not accompanied by any significant differences in the results.


Acrylic dentures; Disinfection; Cold plasma; ASEPTOPRINT® spray


Gupta S, Rani S, Garg S. Infection control knowledge and practice: A cross-sectional survey on dental laboratories in dental institutes of North India. J Indian Prosthodont Soc. 2017;17:348-54. doi: 10.4103/jips.jips_5_17

Bhat VS, Shetty MS, Shenoy KK. Infection control in the prosthodontic laboratory. J Indian Prosthodont Soc. 2007;7(2):62‑5. doi: 10.4103/0972-4052.33997

Kumari KV, Subhash A. Infection Control in Dental Clinic and Laboratory. Indian Journal of Mednodent and Allied Sciences. 2014;2(2). doi: 10.5958/2347-6206.2014.00009.0

Williams DW, Chamary N, Lewis MA, Milward PJ, McAndrew R. Microbial contamination of removable prosthodontic appliances from laboratories and impact of clinical storage. Br Dent J. 2011;211(4):163-6. doi: 10.1038/sj.bdj.2011.675

Al Mortadi N, Al-Khatib A, Alzoubi KH, Khabour OF. Disinfection of dental impressions: knowledge and practice among dental technicians. Clin Cosmet Investig Dent. 2019;11:103-8. doi: 10.2147/CCIDE.S205144

Centers for Disease Control and Prevention. Summary of Infection Prevention Practices in Dental Settings: Basic Expectations for Safe Care. Atlanta, GA: Centers for Disease Control and Prevention, US Dept of Health and Human Services; 2016.

British Dental Association. Health Technical Memorandum 01-05: Decontamination in Primary Care Dental Practices. London: Department of Health; 2013.

ADA Council on Scientific Affairs and ADA Council on Dental Practice. Infection control recommendations for

the dental office and the dental laboratory. J Am Dent Assoc. 1996; 127:672-80.

Carvalho CF, Vanderlei AD, Marocho SM, Pereira SM, Nogueira L, Paes-Junior TJ. Effect of disinfectant solutions on a denture base acrylic resin. Acta Odontol Latinoam. 2012;25(3):255-60.

Aslanimehr M, Mojarad N, Ranjbar S, Aalaei S. In vitro comparison of the effects of microwave irradiation and chemical and mechanical methods on the disinfection of complete dentures contaminated with Candida albicans. Dent Res J (Isfahan). 2018;15(5):340-6. doi: 10.4103/1735-3327.240477

Pavarina AC, Pizzolitto AC, Machado AL, Vergani CE, Giampaolo ET. An infection control protocol: effectiveness of immersion solutions to reduce the microbial growth on dental prostheses. J Oral Rehabil. 2003;30(5):532-6. doi: 10.1046/j.1365 2842.2003.01093.x

Orsi IA, Andrade VG. Effect of chemical disinfectants on the transverse strength of heat-polymerized acrylic

resins submitted to mechanical and chemical polishing. J Prosthet Dent. 2004;92(4):382-8. doi: 10.1016/j. prosdent.2004.07.015

Rutala WA, Weber DJ. Selection of the ideal disinfectant. Infect Control Hosp Epidemiol. 2014;35(7):855-65. doi: 10.1086/676877

Pavarina AC, Vergani CE, Machado AL, Giampaolo ET, Teraoka MT. The effect of disinfectant solutions on the hardness of acrylic resin denture teeth. J Oral Rehabil. 2003;30(7):749-52. doi: 10.1046/j.1365-2842.2003.01145.x

Machado AL, Giampaolo ET, Vergani CE, Souza JF. Antimicrobial Activity of Cold Plasma Treatment Jorge JH. Changes in roughness of denture base and reline materials by chemical disinfection or microwave irradiation: surface roughness of denture base and reline materials. J Appl Oral Sci. 2011;19(5):521-8. doi: 10.1590/s1678-77572011000500015

Sagripanti JL, Bonifacino A. Cytotoxicity of liquid disinfectants. Surg Infect (Larchmt). 2000;1(1):3-14. doi: 10.1089/109629600321245

Ziuzina D, Boehm D, Patil S, Cullen P, Bourke P. Cold plasma inactivation of bacterial biofilms and reduction of quorum sensing regulated virulence factors. PloS One. 2015;10(9):e0138209. doi: 10.1371/journal.pone.0138209

Kim B, Yun H, Jung S, Jung Y, Jung H, Choe W, et al. Effect of atmospheric pressure plasma on inactivation of pathogens inoculated onto bacon using two different gas compositions. Food Microbiol. 2011;28(1):9-13. doi: 10.1016/j.fm.2010.07.022

Song HP, Kim B, Choe JH, Jung S, Moon SY, Choe W, et al. Evaluation of atmospheric pressure plasma to improve the safety of sliced cheese and ham inoculated by 3-strain cocktail Listeria monocytogenes. Food Microbiol. 2009;26(4):432-6. doi: 10.1016/j.fm.2009.02.010

Chen YJ, Chen YC, Chung WC, Tong BS, Chang MB. Evaluation of the effectiveness of nonthermal plasma

disinfection. Environ Technol. 2019:In Press. doi:10.1080/09593330.2019.1583289

Ulbin-Figlewicz N, Jarmoluk A, Marycz K. Antimicrobial activity of low-pressure plasma treatment against selected foodborne bacteria and meat microbiota. Ann Microbiol. 2015;65(3):1537-46. doi: 10.1007/s13213-014-0992-y

Borges AC, Lima GMG, Nishime TMC, Gontijo AVL, Kostov KG, Koga-Ito CY. Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study. PLoS One. 2018;13(6):e0199832. doi: 10.1371/journal.pone.0199832

Moisan M, Barbeau J, Crevier M-C, Pelletier J, Philip N, Saoudi B. Plasma sterilization. Methods and mechanisms. Pure Appl Chem. 2002;74(3):349-58. doi: 10.1351/pac200274030349

Zamperini CA, Machado AL, Vergani CE, Pavarina AC, Giampaolo ET, da Cruz NC. Adherence in vitro of Candida albicans to plasma treated acrylic resin. Effect of plasma parameters, surface roughness and salivary

pellicle. Arch Oral Biol. 2010;55(10):763-70. doi: 10.1016/j.archoralbio.2010.06.015

Goree J, Liu B, Drake D, Stoffels E. Killing of S. mutans bacteria using a plasma needle at atmospheric pressure.

IEEE Trans Plasma Sci. 2006;34(4):1317-24. doi: 10.1109/ TPS.2006.878431

Yang B, Chen J, Yu Q, Li H, Lin M, Mustapha A, et al. Oral bacterial deactivation using a low-temperature atmospheric argon plasma brush. Journal of dentistry. J Dent. 2011;39(1):48-56. doi: 10.1016/j.jdent.2010.10.002

Jiang C, Chen MT, Gorur A, Schaudinn C, Jaramillo DE, Costerton JW, et al. Nanosecond pulsed plasma dental

probe. Plasma Process Polym. 2009;6(8):479-83. doi:10.1002/ppap.200800133

DOI: https://doi.org/10.22037/jlms.v10i4.27368