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The Combined Effects of Mesenchymal Stem Cell Conditioned Media and Low-Level Laser on Stereological and Biomechanical Parameter in Hypothyroidism Rat Model

Niloofar Sefati, Hojjat allah Abbaszadeh, Fatemeh Fadaei Fathabady, Mohammad Amin Abdolahifar, Maraym-Sadat Khoramgah, Shahram Darabi, Abdollah Amini, Foozhan Tahmasebinia, Mohsen Norozian
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Abstract

Introduction: Many studies have shown the positive effect of laser radiation and application of the mesenchymal stem cells (MSCs) and their secretion in stimulating bone regeneration. The aim of this study was determining effects of MSC conditioned media (CM) and low-level laser (LLL) on healing bone defects in the hypothyroid male rat.
Methods: We assigned 30 male Wistar rats randomly to 3 groups: control, hypothyroidism, CM+LLL. Four weeks after surgery, the right tibia was removed. Biomechanical examination and histological examinations were performed immediately.
Results: Our results showed significant increase in bending stiffness (116.09 ± 18.49), maximum force (65.41 ± 8.16), stress high load (23.30 ± 7.14), energy absorption (34.57 ± 4.10), trabecular bone volume (1.34 ± 0.38) and the number of osteocyte, osteoblast, and osteoclast (12.77 ± 0.54, 6.19 ± 0.80, 1.12 ± 0.16 respectively) in osteotomy site in the LLL + CM group compared to the hypothyroidism group (P < 0.05).
Conclusion: The results indicated that using the LLL + CM may improve fracture regeneration and it may hasten bone healing in the hypothyroid rat.

Keywords

Hypothyroidism, low-level laser, condition Media,BMSC

References

Wolf M, Ingbar SH, Moses AC. Thyroid hormone and growth hormone interact to regulate insulin-like growth factor-I messenger ribonucleic acid and circulating levels in the rat. Endocrinology. 1989;125(6):2905-14.

Kindblom J, Gothe S, Forrest D, Tornell J, Vennstrom B, Ohlsson C. GH substitution reverses the growth phenotype but not the defective ossification in thyroid hormone receptor alpha 1-/-beta-/-mice. Journal of Endocrinology. 2001;171(1):15-22.

Sefati N, Norouzian M, Abbaszadeh H, Abdollahifar M, Amini A, Bagheri M, et al . Effects of Bone Marrow Mesenchymal Stem Cells-Conditioned Medium on Tibial Partial Osteotomy Model of Fracture Healing in Hypothyroidism Rats. IBJ. 2018; 22 (2) :90-98

Freitas FR, Capelo LP, O'Shea PJ, Jorgetti V, Moriscot AS, Scanlan TS, et al. The Thyroid Hormone Receptor β‐Specific Agonist GC‐1 Selectively Affects the Bone Development of Hypothyroid Rats. Journal of bone and mineral research. 2005;20(2):294-304.

Sefati N, Abbaszadeh HA, Fadaei Fathabady F, Abdollahifar M, Amini A, Norouzian M, et al .Therapeutic effects of laser on partial osteotomy in the rat model of hypothyroidism. J Lasers Med Sci. 2018 Spring;9(2):143-149.

Mohsenifar Z, Fridoni M, Ghatrehsamani M, Abdollahifar MA, Abbaszadeh HA, Mostafavinia A, et al. Evaluation of the effects of pulsed wave LLLT on tibial diaphysis in two rat models of experimental osteoporosis, as examined by stereological and real-time PCR gene expression analyses. Lasers Med Sci. 2016; 31(4):721–32.

Gao X, Xing D. Molecular mechanisms of cell proliferation induced by low power laser irradiation. Journal of biomedical science. 2009;16(1):4.

Bolognani L, Volpi N. Low power laser enzymology: Reactivation of myosin ATPase by GaAs an HeNe lasers. Basic and Applied Research in Photobiology and Photomedicine’’(S Passarella and E Quagliariello, Eds). 1991:315-38.

Amini A, Pouriran R, Abdollahifar M-A, Abbaszadeh HA, Ghoreishi SK,et al. Stereological and molecular studies on the combined effects of photobiomodulation and human bone marrow mesenchymal stem cell conditioned medium on wound healing in diabetic rats. Journal of Photochemistry and Photobiology B: Biology. Volume 182, May 2018, Pages 42-51.

Abbaszadeh HA, Peyvandi AA, Sadeghi Y, Safaei A, Zamanian-Azodi M, Khoramgah MS, Rezaei-Tavirani M.Er:YAG Laser and Cyclosporin A Effect on Cell Cycle Regulation of Human Gingival Fibroblast Cells.J Lasers Med Sci. 2017 Summer;8(3):143-149.

Kassem M, Kristiansen M, Abdallah BM. Mesenchymal stem cells: cell biology and potential use in therapy. Basic & clinical pharmacology & toxicology. 2004;95(5):209-14.

Peyvandi AA, Abbaszadeh HA, Roozbahany NA, Pourbakht A, Khoshsirat S, Niri HH, Peyvandi H, Niknazar S. Deferoxamine promotes mesenchymal stem cell homing in noise-induced injured cochlea through PI3K/AKT pathway. Cell Prolif. 2018 Apr;51(2):e12434.

Abbaszadeh HA, Tiraihi T, Delshad A, Saghedizadeh M, Taheri T, Kazemi H, et al. Differentiation of neurosphere-derived rat neural stem cells into oligodendrocyte-like cells by repressing PDGF-α and Olig2 with triiodothyronine. Tissue and Cell. 2014;46(6):462-9

Abbaszadeh H, Tiraihi T, Delshad A R, Saghedi Zadeh M, Taheri T. Bone Marrow Stromal Cell Transdifferentiation into Oligodendrocyte-Like Cells Using Triiodothyronine as a Inducer with Expression of Platelet-Derived Growth Factor α as a Maturity Marker. IBJ. 2013; 17 (2) :62-70.

Abbaszadeh H A, Tiraihi T, Sadeghi Y, Delshad A R, Sadeghizadeh M, Taheri T et al . Decrease in Cavity Size and Oligodendrocyte Cell Death Using Neurosphere-Derived Oligodendrocyte-Like Cells in Spinal Cord Contusion Model . IBJ. 2018; 22 (3) :90-98.

J Salgado A, L Reis R, Sousa N, M Gimble J. Adipose tissue derived stem cells secretome: soluble factors and their roles in regenerative medicine. Current stem cell research & therapy. 2010;5(2):103-10.

Hao P, Liang Z, Piao H, Ji X, Wang Y, Liu Y, et al. Conditioned medium of human adipose-derived mesenchymal stem cells mediates protection in neurons following glutamate excitotoxicity by regulating energy metabolism and GAP-43 expression. Metabolic brain disease. 2014;29(1):193-205.

Linero I, Chaparro O. Paracrine effect of mesenchymal stem cells derived from human adipose tissue in bone regeneration. PLoS One. 2014;9(9):e107001.

Nakano N, Nakai Y, Seo T-B, Yamada Y, Ohno T, Yamanaka A, et al. Characterization of conditioned medium of cultured bone marrow stromal cells. Neuroscience letters. 2010;483(1):57-61.

Horie M, Choi H, Lee RH, Reger RL, Ylostalo J, Muneta T, et al. Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen. Osteoarthritis and Cartilage. 2012;20(10):1197-207.

de Almeida PE, Ransohoff JD, Nahid A, Wu JC. Immunogenicity of pluripotent stem cells and their derivatives. Circulation research. 2013;112(3):549-61.

Garavello I, Baranauskas V, da Cruz-Hofling MA. The effects of low laser irradiation on angiogenesis in injured rat tibiae. Histol Histopathol. 2004;19(1):43-48. doi:10.14670/hh-19.43

Sanchooli T, Norouzian M, Ardeshirylajimi A, et al. Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats- Iranian Red Crescent Medical Journal, 2017.




DOI: https://doi.org/10.22037/jlms.v9i4.20867