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Comparative Evaluation of the efficacy of Laser Therapy and Fibroblastic Growth Factor Injection on Mucosal Wound Healing in Rat Experimental Model

Maryam Allameh, Saeedeh Khalesi, Faezeh Khozeimeh, Elham Faghihian
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Abstract


Introduction: The aim of present study was to compare the effects of laser and bFGF treatment on operative wound healing in a rat model.

Methods: 66 male Wistar rats were employed in this study. 10millimeter surgical wound was created on buccal mucosa of each rat ,under anesthesia, and then the rats were divided into 3 groups of 22 each: 1- GF group (received subcutaneous injection of bFGF), 2- laser group (treated with low level laser irradiation) and 3- control group (received no treatment). On day 5 half of rats in each group and on day 10 the other half, were sacrificed. Then, samples were taken from rats' buccal mucosa for histological assay and scoring. The data was analyzed with Mann-Whitney test (α=5%).

Results: On day5 there was not any significant difference between GF and control group; however laser group showed delayed wound coverage clinically, compared to other groups(p<0.05). On day 10, histological examination demonstrated marked vascular granulation tissue in GF group. Collagen production was significantly prominent in laser group compared to GF treated samples (p=.004). Inflammation of granulation tissue in GF and laser groups was significantly less than that in control samples (p=.005 and .001, respectively). 

Conclusion: The components of wound matrix induced by GF and laser treatment were significantly different. Although bFGF or laser treatment of oral wounds, under conditions of present study, did not accelerated wound healing but showed some other notable effects on the quality of healing.


Keywords

bFGF; granulation tissue; laser; oral; wound healing

References

Paleri V, Staines K, Sloan P, Douglas A, Wilson J. Evaluation of oral ulceration in primary care. BMJ. 2010;340:c2639. doi:10.1136/bmj.c2639

Siu A, Landon K, Ramos DM. Differential diagnosis and management of oral ulcers. Semin Cutan Med Surg. 2015;34(4):171-177. doi:10.12788/j.sder.2015.0170

Edgar NR, Saleh D, Miller RA. Recurrent Aphthous Stomatitis: A Review. J Clin Aesthet Dermatol. 2017;10(3):26-36.

Mortazavi H, Safi Y, Baharvand M, Rahmani S. Diagnostic Features of Common Oral Ulcerative Lesions: An Updated Decision Tree. Int J Dent. 2016;2016:7278925. doi:10.1155/2016/7278925

Janeway CA Jr, Travers P, Walport M, Shlomchik MJ. Immunobiology: The Immune System in Health and Disease. 5th ed. New York: Garland Science; 2001.

Rocha Junior AM, Vieira BJ, de Andrade LC, Aarestrup FM. Effects of low-level laser therapy on the progress of wound healing in humans: the contribution of in vitro and in vivo experimental studies. J Vasc Bras. 2007;6(3):258- 266. doi:10.1590/S1677-54492007000300009

Cohen IK, Diegelmann RF, Johnson ML. Effect of corticosteroids on collagen synthesis. Surgery. 1977;82(1):15-20.

Solmaz H, Ulgen Y, Gulsoy M. Photobiomodulation of wound healing via visible and infrared laser irradiation. Lasers Med Sci. 2017;32(4):903-910. doi:10.1007/s10103- 017-2191-0

Rajendran R. Shafer’s Textbook of Oral Pathology. [S.l.]: Reed Elsevier; 2010.

Little JW. Little And Falace’s Dental Management Of The Medically Compromised Patient. [S.l.]: Mosby; 2017.

Drugs that delay wound healing. Prescrire Int. 2013;22(137):94-98.

Paocharoen V. The efficacy and side effects of oral Centella asiatica extract for wound healing promotion in diabetic wound patients. J Med Assoc Thai. 2010;93 Suppl 7:S166- 170.

Samarghandian S, Farkhondeh T, Samini F. Honey and Health: A Review of Recent Clinical Research. Pharmacognosy Res. 2017;9(2):121-127. doi:10.4103/0974- 8490.204647

Beigom Taheri J, Bagheri F, Mojahedi M, et al. Comparison of the Effect of Low-Level Laser and Phenytoin Therapy on Skin Wound Healing in Rats. J Lasers Med Sci. 2015;6(3):124-128. doi:10.15171/jlms.2015.06

Mester E, Nagylucskay S, Doklen A, Tisza S. Laser stimulation of wound healing. Acta Chir Acad Sci Hung. 1976;17(1):49-55.

Aggarwal H, Singh MP, Nahar P, Mathur H, Gv S. Efficacy of low-level laser therapy in treatment of recurrent aphthous ulcers - a sham controlled, split mouth follow up study. J Clin Diagn Res. 2014;8(2):218-221. doi:10.7860/ jcdr/2014/7639.4064

Simunovic Z, Ivankovich AD, Depolo A. Wound healing of animal and human body sport and traffic accident injuries using low-level laser therapy treatment: a randomized clinical study of seventy-four patients with control group. J Clin Laser Med Surg. 2000;18(2):67-73. doi:10.1089/ clm.2000.18.67

Hopkins JT, McLoda TA, Seegmiller JG, David Baxter G. Low-Level Laser Therapy Facilitates Superficial Wound Healing in Humans: A Triple-Blind, Sham-Controlled Study. J Athl Train. 2004;39(3):223-229.

Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M. Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg. 2005;31(3):334-340.

Lucas C, Criens-Poublon LJ, Cockrell CT, de Haan RJ. Wound healing in cell studies and animal model experiments by Low Level Laser Therapy; were clinical studies justified? a systematic review. Lasers Med Sci. 2002;17(2):110-134.

Yasukawa A, Hrui H, Koyama Y, Nagai M, Takakuda K. The effect of low reactive-level laser therapy (LLLT) with helium-neon laser on operative wound healing in a rat model. J Vet Med Sci. 2007;69(8):799-806.

Roberts AB. Transforming growth factor-beta: activity and efficacy in animal models of wound healing. Wound Repair Regen. 1995;3(4):408-418. doi:10.1046/j.1524- 475X.1995.30405.x

Pierce GF, Tarpley JE, Yanagihara D, Mustoe TA, Fox GM, Thomason A. Platelet-derived growth factor (BB homodimer), transforming growth factor-beta 1, and basic fibroblast growth factor in dermal wound healing. Neovessel and matrix formation and cessation of repair. Am J Pathol. 1992;140(6):1375-1388.

Greenhalgh DG, Sprugel KH, Murray MJ, Ross R. PDGF and FGF stimulate wound healing in the genetically diabetic mouse. Am J Pathol. 1990;136(6):1235-1246.

Fujisawa K, Miyamoto Y, Nagayama M. Basic fibroblast growth factor and epidermal growth factor reverse impaired ulcer healing of the rabbit oral mucosa. J Oral Pathol Med. 2003;32(6):358-366.

Szpaderska AM, Zuckerman JD, DiPietro LA. Differential injury responses in oral mucosal and cutaneous wounds. J Dent Res. 2003;82(8):621-626. doi:10.1177/154405910308200810

Oda Y, Kagami H, Ueda M. Accelerating effects of basic fibroblast growth factor on wound healing of rat palatal mucosa. J Oral Maxillofac Surg. 2004;62(1):73-80.

Tabakoglu HO, Sani MM, Uba AI, Abdullahi UA. Assessment of circular wound healing in rats after exposure to 808-nm laser pulses during specific healing phases. Lasers Surg Med. 2016;48(4):409-415. doi:10.1002/ lsm.22462

Fulop AM, Dhimmer S, Deluca JR, et al. A meta-analysis of the efficacy of phototherapy in tissue repair. Photomed Laser Surg. 2009;27(5):695-702. doi:10.1089/pho.2009.2550

Woodruff LD, Bounkeo JM, Brannon WM, et al. The efficacy of laser therapy in wound repair: a meta-analysis of the literature. Photomed Laser Surg. 2004;22(3):241-247. doi:10.1089/1549541041438623

Lins RD, Dantas EM, Lucena KC, Catao MH, Granville- Garcia AF, Carvalho Neto LG. Biostimulation effects of low-power laser in the repair process. An Bras Dermatol. 2010;85(6):849-855.

Berry DP, Harding KG, Stanton MR, Jasani B, Ehrlich HP. Human wound contraction: collagen organization, fibroblasts, and myofibroblasts. Plast Reconstr Surg. 1998;102(1):124-131; discussion 132-124.

Suter VGA, Sjolund S, Bornstein MM. Effect of laser on pain relief and wound healing of recurrent aphthous stomatitis: a systematic review. Lasers Med Sci. 2017;32(4):953-963. doi:10.1007/s10103-017-2184-z