Comparative Study Between Low Level Laser and Therapeutic Ultrasound in Second Intention Ulcers Repair in Mice
Journal of Lasers in Medical Sciences,
Vol. 9 No. 2 (2018),
27 April 2018
,
Page 134-138
Abstract
Introduction: An ulcer is an injury that affects the skin and has many causes. Healing is a way of protecting the body against any infectious agent that tries to infect you through the wound. Low level laser (LLL) in the treatment of ulcers, aims at maintaining the stability of the physiological process of tissue repair and ultrasound (US) acts by increasing the elasticity of tissue and scarring. The aim of this study was to analyze the effects of LLL and US in wound healing of ulcers induced in mice.Methods: In this experimental study, a sample of 36 guinea pigs was divided into 6 groups of 6 mice, 2 groups as control (C7) and (C14), 2 treated with laser (GL7) and (GL14) and the others 2 treated with ultrasound (GU7) and (GU14). The ulcer was induced and after 7 days, 6 mice from each group were sacrificed to obtain the histological sections for analysis, and the remaining 6 mice from each group continued under treatment and in day 14, they were sacrificed to obtain histologic specimens while macroscopic image was also carried out.
Results: Three aspects were analyzed, the percentage of wound regression where it was observed that there was a significant difference in the first 7 days. Regarding the inflammatory process, it was observed that in the first 7 days GL7 and GU7 improved significantly and within 14 days of the experimental period GU14 showed a significant difference when compared to C14. The number of fibroblasts present in the GL at 7 days showed a significant difference compared to the others, and at 14 days, the US group had a significant difference compared to the other groups.
Conclusion: Thus, it was evident that the US had more effective results with anti-inflammatory action, better organization and increased deposition of collagen and fibroblasts. Therefore, it can be concluded that other studies are consistent with this taking into account the laser must be used during the early days of the initial healing process and the US during the end of this process.
- Ulcer
- Healing
- Laser
- Ultrasound.
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References
Peplow PV, Chung TY, Ryan B, Baxter GD. Laser photobiomodulation of gene expression and release of growth factors and cytokines from cells in culture: a review of human and animal studies. Photomed Laser Surg. 2011;29(5):285-304. doi: 10.1089/pho.2010.2846.
Oliveira IVPM, DIAS RVC. Cicatrização de feridas: fases e fatores de influência. Acta Veterinaria Brasilica. 2012;6(4):267-271.
Amâncio ACG, Barbieri CH, Mazzer N, Garcia SB, Thomazini JA. Estimulação ultra-sônica da integração de enxertos de pele total: estudo experimental em coelhos (Ultrasonic stimulation of total skin grafts union. an experimental study in rabbits). Acta Ortop Bras. 2006;14(5):276-279. doi:10.1590/S1413- 78522006000500010.
Beduschi CD, Weiss C, Wolf LS. Transdução da energia sonora para sinais elétricos utilizando material piezoelétrico. http://repositorio.roca.utfpr.edu.br/jspui/ handle/1/3206. Published 2015.
Silva EM, Gomes SP, Ulbrich LM, Giovanini AF. Avaliação histológica da laserterapia de baixa intensidade na cicatrização de tecidos epitelial, conjuntivo e ósseo: estudo experimental em ratos (Histological evaluation of the effects of LLLT on the epithelial, connective and osseous tissue healing: experimental study in rats). Revista Sul- Brasileira de Odontologia. 2007;4(2):30-35.
Lins RDAU, Dantas EM, Lucena KCR, Catão MHCV, Granville-Garcia AF, Carvalho Neto LG. Efeitos bioestimulantes do laser de baixa potência no processo de reparo (Biostimulation effects of low-power laser in the repair process). Anais Brasileiros de Dermatologia. 2010;85:849-855.
Cristina E. Laser de baixa potência: um forte aliado ao tratamento cicatricial da úlcera de pressão em pacientes hospitalizados. Rev Min Ciência da Saúde. 2011;(3):35–42.
Moreira DO, Campmany WG. Cura e reparo induzida por laserterapia: revisão bibliográfica. 25p. Trabalho de conclusão de curso. Universidade São Francisco – Bragança Paulista; 2015.
Vieira MB, Ortiz DA. Alicação do laser AlGaInP de 660 nm em úlcera de pressão grau 3: Relato de caso. 2010.
Ramires RC, Meija DPM. Os efeitos do laser no tratamento de úlcera de decúbito–Revisão Bibliográfica. Fac Ávila-Ter Man. 2012.
FREIRE, M. C. G. S. et al. Estudo histológico comparativo entre laser de baixa potência, própolis e associação de ambas sobre lesões de pele de Rattusnorvegicus albinos [dissertation]. Alfenas: Universidade José do Rosário Vellano; 2010.
Ayuk SM, Houreld NN, Abrahamse H. Collagen production in diabetic wounded fibroblasts in response to low-intensity laser irradiation at 660 nm. Diabetes Technol Ther. 2012;14(12):1110-1117. doi: 10.1089/dia.2012.0125.
Sanati MH, Torkaman G, Hedayati M, Dizaji MM. Effect of Ga-As (904 nm) and He-Ne (632.8 nm) laser on injury potential of skin full-thickness wound. J Photochem Photobiol B. 2011;103(2):180-5. doi: 10.1016/j. jphotobiol.2011.03.003.
Leite SN. Fototerapia como estímulo à cicatrização de úlceras cutâneas em ratos frente ao estresse oxidativo experimental. Rev Bras Ciências Farm. 2014.
Martins BS, Portolez JL, Bertolini G. Estudo comparativo entre o efeito do Ultra-som terapêutico e do laser de Baixa potência em lesões tendinosas Induzidas em ratos. Revista UNILUS Ensino e Pesquisa. 2014;11(22):77-83.
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