Comparison of Er:YAG Laser and Hand Instrumentation on the Attachment of Cultured Human Gingival Fibroblasts to Periodontally Involved Root Surfaces

Mohammad Reza Talebi-Ardakani--- Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran,
Maryam Torshabi--- Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran,
Elahe Karami--- Department of Periodontics, School of Dentistry, Yazd University of Medical Sciences, Yazd, Iran,
Nazanin Vajar--- School of Dentistry, International Branch, Shahid Beheshti University of Medical Sciences, Tehran, Iran,
zeinab rezaei esfahrood--- Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran,
Mansour Meimandi--- Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran,
Seyed Masoud Mojahedi--- RWTH University, Aachen, Germany

Abstract


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Background

The present study compared the effects of Er:YAG laser and hand instrumentation on the attachment of human gingival fibroblast (HGF) cells to periodontally involved root surfaces.

Methods

A total of 40 tooth specimens were collected and treated in four distinct groups: scaled and root planed with hand instruments, treated with Er:YAG laser, treated with combination of hand instruments and Er:YAG laser and non-treated control group. The attachment and proliferation rate of HGF were assessed using MTT assay and the Scanning Electron Microscope (SEM) Examination was used for cell morphological evaluation.

Results

The MTT assay showed significantly decrease in HGF cell viability in both hand instruments only and combination treated teeth specimens compared to control specimens (p<0.05), 24 hours after cell seeding. However, at time 48, the cell viability of attached cells in these two treated groups was almost similar to control.  In contrast, at 24 and 48 hours after cell seeding, viability of attached cells was higher than control in Er:YAG laser treated only specimens (p<0.05). According to SEM study, the laser treated specimens showed more surface roughness.

Conclusions

Er:YAG laser increased attachment and proliferation of HGF cells in comparison with hand instruments method.


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DOI: http://dx.doi.org/10.22037/2010.v8i3.10373

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