The present study compared the effects of Er:YAG laser and hand instrumentation on the attachment of human gingival fibroblast (HGF) cells to periodontally involved root surfaces.
A total of 40 tooth specimens were collected and treated in four distinct groups: scaled and root planed with hand instruments, treated with Er:YAG laser, treated with combination of hand instruments and Er:YAG laser and non-treated control group. The attachment and proliferation rate of HGF were assessed using MTT assay and the Scanning Electron Microscope (SEM) Examination was used for cell morphological evaluation.
The MTT assay showed significantly decrease in HGF cell viability in both hand instruments only and combination treated teeth specimens compared to control specimens (p<0.05), 24 hours after cell seeding. However, at time 48, the cell viability of attached cells in these two treated groups was almost similar to control. In contrast, at 24 and 48 hours after cell seeding, viability of attached cells was higher than control in Er:YAG laser treated only specimens (p<0.05). According to SEM study, the laser treated specimens showed more surface roughness.
Er:YAG laser increased attachment and proliferation of HGF cells in comparison with hand instruments method.
O'Leary TJ. The impact of research on scaling and root planing. J Periodontol 1986;57(2):69-75.
Kepic TJ, O'Leary TJ, Kafrawy AH. Total calculus removal: an attainable objective? J Periodontol 1990;61(1):16-20.
Badersten A, Nilveus R, Egelberg J. Effect of nonsurgical periodontal therapy. II. Severely advanced periodontitis. J Clin Periodontol 1984;11(1):63-76.
Tunkel J, Heinecke A, Flemmig TF. A systematic review of efficacy of machine-driven and manual subgingival debridement in the treatment of chronic periodontitis. J Clin Periodontol 2002;29 Suppl 3:72-81; discussion 90-1.
O'Leary TJ, Kafrawy AH. Total cementum removal: a realistic objective? J Periodontol 1983;54(4):221-6.
Adriaens PA, Edwards CA, De Boever JA, Loesche WJ. Ultrastructural observations on bacterial invasion in cementum and radicular dentin of periodontally diseased human teeth. J Periodontol 1988;59(8):493-503.
Oda S, Nitta H, Setoguchi T, Izumi Y, Ishikawa I. Current concepts and advances in manual and power-driven instrumentation. Periodontol 2000 2004;36:45-58.
Polson AM, Frederick GT, Ladenheim S, Hanes PJ. The production of a root surface smear layer by instrumentation and its removal by citric acid. J Periodontol 1984;55(8):443-6.
Aleo JJ, De Renzis FA, Farber PA, Varboncoeur AP. The presence and biologic activity of cementum-bound endotoxin. J Periodontol 1974;45(9):672-5.
Aoki A, Sasaki KM, Watanabe H, Ishikawa I. Lasers in nonsurgical periodontal therapy. Periodontol 2000 2004;36:59-97.
Aoki A, Ando Y, Watanabe H, Ishikawa I. In vitro studies on laser scaling of subgingival calculus with an erbium:YAG laser. J Periodontol 1994;65(12):1097-106.
Folwaczny M, Mehl A, Haffner C, Benz C, Hickel R. Root substance removal with Er:YAG laser radiation at different parameters using a new delivery system. J Periodontol 2000;71(2):147-55.
Israel M, Cobb CM, Rossmann JA, Spencer P. The effects of CO2, Nd:YAG and Er:YAG lasers with and without surface coolant on tooth root surfaces. An in vitro study. J Clin Periodontol 1997;24(9 Pt 1):595-602.
Schwarz F, Putz N, Georg T, Reich E. Effect of an Er:YAG laser on periodontally involved root surfaces: an in vivo and in vitro SEM comparison. Lasers in surgery and medicine. Lasers Surg Med 2001;29(4):328-35.
Hatfield CG, Baumhammers A. Cytotoxic effects of periodontally involved surfaces of human teeth. Arch Oral Biol 1971;16(4):465-8.
Waerhaug J. Healing of the dento-epithelial junction following subgingival plaque control. II: As observed on extracted teeth. J Periodontol. 1978;49(3):119-34.
Sasaki KM, Aoki A, Ichinose S, Ishikawa I. Morphological analysis of cementum and root dentin after Er:YAG laser irradiation. Lasers Surg Med 2002;31(2):79-85.
Crespi R, Romanos GE, Barone A, Sculean A, Covani U. Er:YAG laser in defocused mode for scaling of periodontally involved root surfaces: an in vitro pilot study. J Periodontol 2005;76(5):686-90.
Gomez C, Costela A, Garcia-Moreno I, Garcia JA. In vitro evaluation of Nd:YAG laser radiation at three different wavelengths (1064, 532, and 355 nm) on calculus removal in comparison with ultrasonic scaling. Photomed Laser Surg 2006;24(3):366-76.
Barone A, Covani U, Crespi R, Romanos GE. Root surface morphological changes after focused versus defocused CO2 laser irradiation: a scanning electron microscopy analysis. J Periodontol 2002;73(4):370-3.
Morlock BJ, Pippin DJ, Cobb CM, Killoy WJ, Rapley JW. The effect of Nd:YAG laser exposure on root surfaces when used as an adjunct to root planing: an in vitro study. J Periodontol 1992;63(7):637-41.
Mindermann A, Niemz MH, Eisenmann L, Loesel FH, Bille JF. Comparison of three different laser systems for application in dentistry. Dental Applications of Lasers 1993; 68 (31).
Keller U, Hibst R. Lasers in dentistry: clinical application today and tomorrow. Dental Applications of Lasers 1993; 2 (31).
Aoki A, Ishikawa I, Yamada T, et al. Comparison between Er:YAG laser and conventional technique for root caries treatment in vitro. J Dent Res 1998;77(6):1404-14.
Bolortuya G, Ebihara A, Ichinose S, et al. Effects of dentin surface modifications treated with Er:YAG and Nd:YAG laser irradiation on fibroblast cell adhesion. Photomed Laser Surg 2012;30(2):63-70.
Brunette DM. The effects of implant surface topography on the behavior of cells. Int J Oral Maxillofac Implants 1988;3(4):231-46.
Babay N. Attachment of human gingival fibroblasts to periodontally involved root surface following scaling and/or etching procedures: a scanning electron microscopy study. Brazilian dental journal 2001;12(1):17-21.
Fernyhough W, Page RC. Attachment, growth and synthesis by human gingival fibroblasts on demineralized or fibronectin-treated normal and diseased tooth roots. J Periodontol 1983;54(3):133-40.
Pourzarandian A, Watanabe H, Aoki A, et al. Histological and TEM examination of early stages of bone healing after Er:YAG laser irradiation. Photomed Laser Surg 2004;22(4):342-50.
Theodoro LH, Sampaio JE, Haypek P, Bachmann L, Zezell DM, Garcia VG. Effect of Er:YAG and Diode lasers on the adhesion of blood components and on the morphology of irradiated root surfaces. J Periodontal Res 2006;41(5):381-90.
Kononen M, Hormia M, Kivilahti J, Hautaniemi J, Thesleff I. Effect of surface processing on the attachment, orientation, and proliferation of human gingival fibroblasts on titanium. J Biomed Mater Res A 1992;26(10):1325-41.
Bowers KT, Keller JC, Randolph BA, Wick DG, Michaels CM. Optimization of surface micromorphology for enhanced osteoblast responses in vitro. Int J Oral Maxillofac Implants 1992;7(3):302-10.
Schwarz F, Aoki A, Sculean A, Georg T, Scherbaum W, Becker J. In vivo effects of an Er:YAG laser, an ultrasonic system and scaling and root planing on the biocompatibility of periodontally diseased root surfaces in cultures of human PDL fibroblasts. Lasers Surg Med 2003;33(2):140-7.
Feist IS, De Micheli G, Carneiro SR, Eduardo CP, Miyagi S, Marques MM. Adhesion and growth of cultured human gingival fibroblasts on periodontally involved root surfaces treated by Er:YAG laser. J Periodontol 2003;74(9):1368-75.
Crespi R, Romanos GE, Cassinelli C, Gherlone E. Effects of Er:YAG laser and ultrasonic treatment on fibroblast attachment to root surfaces: an in vitro study. J Periodontol 2006;77(7):1217-22.
Maruyama H, Aoki A, Sasaki KM, et al. The effect of chemical and/or mechanical conditioning on the Er:YAG laser-treated root cementum: analysis of surface morphology and periodontal ligament fibroblast attachment. Lasers Surg Med 2008;40(3):211-22.
Cobb CM. Lasers in periodontics: a review of the literature. J Periodontol 2006;77(4):545-64.
Fayad MI, Hawkinson R, Daniel J, Hao J. The effect of CO2 laser irradiation on PDL cell attachment to resected root surfaces. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97(4):518-23.