Does increased Nitric Oxide production and oxidative stress due to high fat diet affect cardiac function after myocardial infarction?

Marjan Aghajani, Alireza Imani, Mahdieh Faghihi, Mohammad Reza Vaez Mahdavi, Sarah Mahboubi, Fatemeh Moradi, Ehsan Kazemi Moghaddam



Background &Objectives: High fat (HF) diet by affecting the oxidative stress and nitric oxide (NO) production may lead to different effects on function of the heart after myocardial infarction (MI). In the present study we aimed to address the hypothesis that high release of NO by activated macrophages affects LV function after MI.

Methods: The animals were randomly divided into four groups comprising each of 10 rats: 1) Sham; 2) MI; 3) Sham+ HF diet; 4) MI+ HF diet. Animals fed with HF diet 30 days before sham and MI surgery. MI was induced by permanent ligation of left anterior descending coronary artery (LAD). Nitric oxide (NO) production of peritoneal macrophages, the concentrations of MDA in the heart and the infarct size were measured.

Results: Our study indicated that HF has adverse effects on myocardium and it may increase NO production as well as oxidative stress, resulting in augmentation of infarct size.

Conclusion: Our results add to our knowledge that HF diet was associated with overproduction of NO by peritoneal macrophages and ROS that lead to development of infarct size and adverse remodeling.


High fat diet, myocardial infarction, Nitric oxide, oxidative stress, peritoneal macrophages

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