Antimicrobial Peptides (AMPs)

Mehrzad Sadredinamin, Faramarz Mehrnejad, Peyman Hosseini, Farahnoosh Doustdar



Antimicrobial peptides (AMPs) are extensive group of molecules that produced by variety tissues of invertebrate, plants, and animal species which play an important role in their immunity response. AMPs have different classifications such as; biosynthetic machines, biological sources, biological functions, molecular properties, covalent bonding patterns, three dimensional structures, and molecular targets.

These molecules have multidimensional properties including antimicrobial activity, antiviral activity, antifungal activity, anti-parasite activity, biofilm control, antitumor activity, mitogens activity and linking innate to adaptive immunity that making them promising agents for therapeutic drugs. In spite of this advantage of AMPs, their clinical developments have some limitation for commercial development. But some of AMPs are under clinical trials for the therapeutic purpose such as diabetic foot ulcers, different bacterial infections and tissue damage. In this review, we emphasized on the source, structure, multidimensional properties, limitation and therapeutic applications of various antimicrobial peptides.


Antimicrobial peptides Drug resistance, Multidimensional properties

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Brogden KA. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria. Nat Rev Microbiol. 2005;3(3):238-50.

Mulder KC, Lima LA, Miranda VJ, Dias SC, Franco OL. Current scenario of peptide-based drugs: the key roles of cationic antitumor and antiviral peptides. Front Microbiol. 2013;31(4):32.

Conlon JM, Mechkarska M. host- defense peptides with therapeutic potential from skin secretions of frogs from the family pipidae. Pharmaceuticals (Basel). 2014;7(1):58-77.

AdemBahar A, Ren D. Antimicrobial Peptides. Pharmaceuticals (Basel). 2013;6(12):1543–75.

Gaspar D, Veiga AS, CastanhoMA.From antimicrobial to anticancer peptides.A review.Front Microbiol. 2013;1(4):294.

Cederlund A, Gudmundsson GH, Agerberth B. Antimicrobial peptides important in innate immunity.FEBS J. 2011;278(20):3942-51.

Wang G. Database-Guided Discovery of Potent Peptides to Combat HIV-1or Superbugs. Pharmaceuticals 2013; 6(6): 728-758.

Mehrnejad F1, Naderi-Manesh H, Ranjbar B, Maroufi B, Asoodeh A, Doustdar F. PCR-based gene synthesis, molecular cloning, high level expression, purification, and characterization of novel antimicrobial peptide, brevinin-2R, in Escherichia coli.ApplBiochemBiotechnol. 2008;149(2):109-18.

Bala P, Kumar J. ANTIMICROBIAL PEPTIDES: A REVIEW. Int. J. LifeSc. Bt& Pharm. Res. 2014;3(1):2250-3137.

Hotchkiss RD, Dubos RJ. Fractionation of the bactericidal agent from cultures of a soil Bacillus. J Biol Chem.. 1940;(132):791–2.

Wang, G (ed.) Antimicrobial Peptides: Discovery, Design and Novel Therapeutic Strategies. CABI, England; 2010.

Wang G. Improved methods for classification, prediction, and design of antimicrobial peptides. Methods Mol. Biol.2015;43-66.

Teixeira V, Feio MJ, Bastos M. Role of lipids in the interaction of antimicrobial peptides with membranes.Prog Lipid Res. 2012;51(2):149-77.

Harris F, Dennison SR, Phoenix DA. Anionic antimicrobial peptides from eukaryotic organisms.Curr Protein Pept Sci. 2009;10(6):585-606.

Peravali JB, Kotra SR, Sobha K, Nelson R, Rajesh KV, Pulicherla KK, Antimicrobial Peptides: An effective alternative for antibiotic therapy. Mintage journal of Pharmaceutical & Medical Sciences. 2013;2(2):1-7.

Brian M. Peters, Mark E. Shirtliff, Jabra-Rizk M. Antimicrobial peptides: primeval Molecules or feuture drugs. PLoS Pathog. 2010;6(10): e1001067.

Guilhelmelli F, Vilela N, Albuquerque P, DerengowskiLda S, Silva-Pereira I, Kyaw CM. Antibiotic development challenges:the various mechanisms of action of antimicrobial peptides and ofbacterial resistance. Front Microbiol. 2013;9(4):353.

Kostakioti M, Hadjifrangiskou M, Hultgren SJ. Bacterial biofilms: development, dispersal, and therapeutic strategies in the dawn of the post antibiotic era. Cold Spring HarbPerspect Med. 2013;3(4):a010306.

Islami M, Mehrnejad F, Doustdar F, Alimohammadi M, Khadem-Maaref M, Mir-Derikvand M, Taghdir M. Study of orientation and penetration of LAH4 into lipid bilayer membranes: pH and composition dependence. Chem Biol Drug Des. 2014;84(2):242-52.

Hoskin DW, Ramamoorthy A. Studies on anticancer activities of antimicrobial peptides.BiochimBiophysActa. 2008;1778(2):357-75.

Murphy CJ, Foster BA, Mannis MJ, Selsted ME, Reid TW. Defensins are mitogenic for epithelial cells and fibroblasts. J Cell Physiol. 1993;155(2):408-13.

Aarbiou J, Ertmann M, van Wetering S, van Noort P, Rook D, Rabe KF, et al. Human neutrophil defensins induce lung epithelial cell proliferation in vitro. J Leukoc Biol. 2002; 72(1):167-74.

Muller C, Markovic-Lipkovski J, Klatt T, Gamper J, Schwarz G, Beck H, et al. Human α-Defensins HNPs-1, -2, and -3 in Renal Cell Carcinoma. 2002;160(4):1311-24.

Kamysz W, Okrój M, Łukasiak J. Novel properties of antimicrobial peptides.ActaBiochim Pol. 2003;50(2):461-9.

Auvynet C, Rosenstein Y. Multifunctional host defense peptides: antimicrobial peptides, the small yet big players in innate and adaptive immunity. FEBS J. 2009;276(22):6497-508.

Oppenheim J, ABiragyn L, Yang D. Roles of antimicrobial peptides such as defensins in innate and adaptive immunity. Ann Rheum Dis. 2003;62(Supple 2):17–21.

Seo M, Won H, Kim J, Mishig-Ochir T, Lee B. Antimicrobial peptides for therapeutic applications: a review. Molecules. 2012;17(10):12276-86.

Giuliani A, Pirri G, Fabiole-Nicoletto S. Antimicrobial peptides: an overview of a promising class of therapeutics. Central European Journal of Biology. 2007;2(1):1–33.

Mehrnejad F, Khadem-Maaref M, Ghahremanpour MM, Doustdar F. Mechanisms of amphipathic helical peptide denaturation by guanidinium chloride and urea: a molecular dynamics simulation study. J Comput Aided Mol Des. 2010;24(10):829-41.

Mehrnejad F, Naderi-Manesh H, Ranjbar B. The structural properties of magainin in water, TFE/water, and aqueous urea solutions: molecular dynamics simulations. Proteins. 2007;67(4):931-40.

Mehrnejad F, Ghahremanpour M, Khadem-Maaref M, Doustdar F. Effects of osmolytes on the helical conformation of model peptide: molecular dynamics simulation. J Chem Phys. 2011;134(3):035104.

Zasloff M. Antimicrobial peptides of multicellular organisms.Nature. 2002; 415(6870):389-95.

Gordon Y, Eric G. A Review of Antimicrobial Peptides and Their Therapeutic Potential as Anti-Infective Drugs. Curr Eye Res. 2005;30(7):505–15.

Fox JL. Antimicrobial peptides stage a comeback. Nat Biotechnol. 2013;31(5):379-82.