Evaluation of Bax and Bcl-2 Proteins Expression in the Rat Hippocampus due to Childhood Febrile Seizure
Iranian Journal of Child Neurology,
Vol. 10 No. 1 (2016),
1 January 2016
,
Page 53-60
https://doi.org/10.22037/ijcn.v10i1.8202
Abstract
How to Cite This Article: Saeedi Borujeni MJ, Hami J, Haghir H, Rastin M, SazegarGh. Evaluation of Bax and Bcl-2 Proteins Expression in the Rat Hippocampus due to childhood Febrile Seizure. Iran J Child Neurol. Winter 2016; 10(1):53-60.
Abstract
Objective
Simple Febrile Seizure (SFS) is the most common seizure disorder in childhood, and is frequently described as inoffensive disorder. Nevertheless, there is evidence suggesting the association between neonatal febrile seizures and hippocampal abnormalities in adulthood. This study was conducted at evaluating the hippocampal expression of pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins following SFS induction in rat neonates.
Materials & Methods
Febrile seizure was modeled by hyperthermia-induced seizure in 22-dayold male rats by a hot water bath. The animals were divided into two groups based on the presence or absence of seizure behaviors: Hyperthermia without seizure (n=10) and hyperthermia with seizure (n=10). To control the effects of environmental stress a sham-control group was also added (n=10). The rats’ hippocampi were dissected 2 or 15 days after hyperthermia. The expression of Bax and Bcl-2 proteins were measured using Western Blotting technique.
Results
The hippocampal expression of Bcl-2 protein was significantly lower in the hyperthermia with seizure animals than that of the sham-control and hyperthermia without seizure groups. The expression of pro-apoptotic Bax protein also significantly increased in the hippocampus of hyperthermia with seizure group rats compared to the sham-control and hyperthermia without seizure animals.
Conclusion
The simple febrile seizure markedly disturbed the hippocampal expression of both Bcl2 and Bax proteins, resulting in apoptosis promotion in hippocampi of juvenile rats, which were measurable for at least 15 days.
- Simple Febrile Seizure
- Hippocampus
- Apoptosis
How to Cite
References
Dalbem JS, Siqueira HH, Espinosa MM, Alvarenga RP. Febrile seizures: a population-based study. J Pediatr 2015. In Press
Cendes F. Febrile seizures and mesial temporal sclerosis. Current Opinion Neurol 2004;17(2):161-4.
Preux P-M, Ratsimbazafy V, Jost J. Epidemiology of febrile seizures and epilepsy: a call for action. J Pediatr 2015.In press
Bender RA, Dube C, Baram TZ. Febrile seizures and mechanisms of epileptogenesis: insights from an animal model. Adv Exp MedBiol 2004;548:213-25.
Lowe A, Dalton M, Sidhu K, Sachdev P, Reynolds B, Valenzuela M. Neurogenesis and precursor cell differences in the dorsal and ventral adult canine hippocampus. Neurosci Let 2015;593:107-13.
Kimia AA, Bachur RG, Torres A, Harper MB. Febrile seizures: emergency medicine perspective. Current Opinion Neurol 2015;27(3):292-7.
Wo SB, Lee JH, Lee YJ, Sung TJ, Lee KH, Kim SK. Risk for developing epilepsy and epileptiform discharges on EEG in patients with febrile seizures. Brain Develop 2013;35(4):307-11.
Auer T, Barsi P, Bone B, Angyalosi A, Aradi M, Szalay C, et al. History of simple febrile seizures is associated with hippocampal abnormalities in adults. Epilepsia 2008;49(9):1562-9.
Kinney HC, Chadwick AE, Crandall LA, Grafe M, Armstrong DL, Kupsky WJ, et al. Sudden death, febrile seizures, and hippocampal and temporal lobe maldevelopment in toddlers: a new entity. Pediatr Develop Pathol 2009;12(6):455-63.
D’Amelio M, Sheng M, Cecconi F. Caspase-3 in the central nervous system: beyond apoptosis. Trends Neurosci 2012;35(11):700-9.
He B, Lu N, Zhou Z. Cellular and nuclear degradation during apoptosisCurrent Opinion Neurol 2009;21(6):900-12.
Renault TT, Floros KV, Elkholi R, Corrigan K-A, Kushnareva Y, Wieder SY, et al. Mitochondrial shape governs BAX-induced membrane permeabilization and apoptosis. Molecular Cell 2015;57(1):69-82.
Martinou JC, Youle RJ. Mitochondria in apoptosis: Bcl-2 family members and mitochondrial dynamics. Develop Cell 2011;21(1):92-101.
Ola MS, Nawaz M, Ahsan H. Role of Bcl-2 family proteins and caspases in the regulation of apoptosis. Molec Cellular Biochem 2011;351(1-2):41-58.
Reed JC. Roles of Apoptosis-Regulating Bcl-2 Family Genes in AML. In: Andreeff M, ed. Targeted Therapy of Acute Myeloid Leukemia. 1st ed. Springer; 2015.P.47-65.
Ates N, Akman O, Karson A. The effects of the immature rat model of febrile seizures on the occurrence of later generalized tonic-clonic and absence epilepsy. Develop Brain Res 2005;154(1):137-40.
Jiang W, Duong TM, de Lanerolle NC. The neuropathology of hyperthermic seizures in the rat. Epilepsia 1999;40(1):5-19.
Nazem A, Jafarian AH, Sadraie SH, Gorji A, Kheradmand H, Radmard M, et al. Neuronal injury and cytogenesis after simple febrile seizures in the hippocampal dentate gyrus of juvenile rat. Child’s Nervous System 2012;28(11):1931-6.
Fewell JE, Wong VH. Interleukin-1beta-induced fever does not alter the ability of 5- to 6-day-old rat pups to autoresuscitate from hypoxia-induced apnoea. Exp Physiol 2002;87(1):17-24.
Gottlieb A, Keydar I, Epstein HT. Rodent brain growth stages: an analytical review. Biology Neonate 1977;32(3-4):166-76.
Depondt C, Van Paesschen W, Matthijs G, Legius E, Martens K, Demaerel P, et al. Familial temporal lobe epilepsy with febrile seizures. Neurology 2002;58(9):1429-33.
Sayin U, Hutchinson E, Meyerand M, Sutula T. Agedependent long-term structural and functional effects of early-life seizures: Evidence for a hippocampal critical period influencing plasticity in adulthood. Neuroscience 2015;288:120-34.
Xiong Y, Zhou H, Zhang L. Influences of hyperthermiainduced seizures on learning, memory and phosphorylative state of CaMKIIα in rat hippocampus. Brain Res 2014;1557:190-200.
Qu L, Leung LS. Effects of temperature elevation on neuronal inhibition in hippocampal neurons of immature and mature rats. J Neurosci Res 2009;87(12):2773-85.
Toth Z, Yan XX, Haftoglou S, Ribak CE, Baram TZ. Seizure-induced neuronal injury: vulnerability to febrile seizures in an immature rat model. J Neurosci 1998;18(11):4285-94.
Xiong Y, Zhou H, Zhang L. Influences of hyperthermiainduced seizures on learning, memory and phosphorylative state of CaMKIIα in rat hippocampus. Brain Res 2014;1557:190-200.
Cassim S, Qulu L, Mabandla MV. Prenatal stress and early life febrile convulsions compromise hippocampal genes MeCP2/REST function in mid-adolescent life of Sprague-Dawley rats. Neurobiol Learning Memory. 2015.In press
Lewis DV, Shinnar S, Hesdorffer DC, Bagiella E, Bello JA, Chan S, et al. Hippocampal sclerosis after febrile status epilepticus: the FEBSTAT study. Ann Neurol 2014;75(2):178-85.
- Abstract Viewed: 818 times