Frequency of Toxoplasma and Toxocara Sp. Antibodies in Epileptic Patients, in South Western Iran
Iranian Journal of Child Neurology,
Vol. 9 No. 4 (2015),
8 Mehr 2015
,
Page 32-40
https://doi.org/10.22037/ijcn.v9i4.7943
Abstract
How to Cite This Article: Allahdin S, Khademvatan S, Rafiei A, Momen AA, Rafiei R. Frequency of Toxoplasma and Toxocara Sp. Antibodies in Epileptic Patients, in South Western Iran. Iran J Child Neurol. Autumn 2015;9(4):32-40.
Abstract
Objective
Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate seizures. Infectious agents are mentioned in its etiology. With identifying and appropriate treatment of these infectious agents, preventing their secondary outcomes, including seizure is possible. This study was conducted to determine frequency of anti-Toxoplasma antibodies (IgG, IgM) and anti- Toxocara antibody (IgG) in epileptic patients.
Materials & Methods
Study sample consisted of 141 epileptic patients and 144 healthy people. After obtaining informed consents and completing demographic questionnaire, serum samples were taken from participants. The diagnostic test of Toxoplasma IgG & IgM and Toxocara antibodies was performed under the same conditions using ELISA method in a qualified private laboratory. Samples from patients and control groups with positive ELISA test in terms of anti-Toxocara antibody were also used for confirmatory Western blot test.
Result
According to ELISA results, 28 (19.85%) epileptic patients and 2(1.38%) of healthy people had anti-Toxocara antibodies (P<001), while 39 (30.46%) of the control group people and 14.18% of patients had anti-Toxoplsma antibodies (P=0.001).
Conclusion
Frequency of anti-Toxoplasma gondii is lower in epileptic than healthy individuals and this result is contrary to investigations that have reported higher levels of this antibody in such patient groups. ELISA results for Toxocara showed that the frequency of anti-Toxocara antibody in epileptic patients might empower the probability that this parasite may cause central nervous system damage. Western
blotting has high specificity and is a proper confirmative method for diagnosis of toxocariasis.
- Epilepsy
- Toxoplasma
- Toxocara
- Seroprevalence
How to Cite
References
Kliegman, Stanton, Geme ST, Schor, Behrman. Nelson ̓s textbook of pediatrics.19 Ed, chapter 586, PP2013.
Sander JW. The epidemiology of epilepsy revisite. Curr Opin Neurol 2003;16:165-170.
Mukhopadhyay HK, Kandar CC, Kumar Das S, Ghosh L, Gupta BK. Epilepsy and its management: A review. JPST 2012;1(2): 20-26.
Wagner RG, Newton CR. Do helminths cause epilepsy? Parasite Immunol 2009;31(11):697-705.
Foyaca-Sibat H. Novel Aspects on Epilepsy. In Tech Publisher 2011; 305-338.
Sander JW, Perucca E. Epilepsy and comorbidity: infections and antimicrobials usage in relation to epilepsy management. Acta Neurol Scand 2003;108: 16-22.
Zibaei M, Zamani Z, ChahichiEsfahani A, Anbari K, Nazer M. Toxoplasma infectionand epilepsy: A casecontrol study in Iran. Neurol Asia 2011; 16(4): 299 – 302.
Yazar S, Arman F, Yalçin s, Demirta F,Yaman O, Shahin I. Investigation of probable relationship between
Toxoplasma gondii and cryptogenic epilepsy. Seizure 2003; 12: 107–109.
Palmer BS. Meta-analysis of Three Case Controlled Studies and an Ecological Study Into the Link Between Cryptogenic Epilepsy and Chronic Toxoplasmosis infection. Seizure 2007; 16(8 ): 657-63.
Nicoletti A, Bartoloni A, Raggio MD, Bartalesi F, Roselli M, Sofia V. Epilepsy, cysticercosis and toxocariasis a population - based case - control study in rural Bolivia. Neurolgy 2002; 58: 1256 -1261.
Finsterer J, Auer H. Neurotoxocarosis. Rev Inst Med Trop Sao Paulo. 2007;49:279-87.
Liao CH, Fan CH, Kao T, ji D, Su K, Lin Y, Cho W. Brain injury - associated biomarker of TGF-beta1, s100B, GFAP, NF-L, tTG, Abeta pp and tau were concomitantly enhanced and the UPS was impaired during acute brain injury caused by Toxocaracanis in mice. BMC Infectious Diseases 2008; 8, 84.
Nicoletti A, Sofia V, Mantella A, Vitale G, Contrafatto D, Sorbello V, Biondi R, Preux PM, Garcia HH, Zappia M, Bartoloni A. Epilepsy and toxocariasis: a case–control study in Italy. Epilepsia 2008; 49(4): 594–599.
Critchley EM, Vakil SD, Hutchinson DN, Taylor P. Toxoplasma, Toxocara and epilepsy. Epilepsia
;23(3): 315-21.
Magnaval j, Glickman l, Dorchies PH, Morassin B. Highlight of human toxocariasis. The Korean J Parasitol 2001;39 (1): 1-11.
Roldan W, Espinoza Y. Evaluation of an enzyme – lined immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis. MemInst Oswaldo Cruz, Rio Del Janeiro 2009; 104 (3): 411- 418.
Hashemi HJ, Saraei M. Seroprevalence of Toxoplasma Gondii in unmarried women in Qazvin, Islamic Republic of Iran. East Mediterr Health J 2010;16: 24-28.
Quattrocchi G, Nicoletti A, marin B, Bruno E, Cabanac M, Preux P. Toxocariasis and epilepsy: Systematic review and meta – analysis. Plos Neg Trop Dis 2012;6(8),e1775.
Swaiman K F, Ferriero D M, Ashwal S, Shor N F.Swaiman ̓s pediatric neurology. Fifth Eddition, chapter 50, pp 704-705.
Zibaei M, Firoozeh F, Bahrami P, Sadjjadi S M. Investigation of Anti-Toxocara antibodies in epileptic
patients and comparison of two methods: Elisa and Western blotting. Hindawi publishing corporation
epilepsy research and treatment 2013; 5pages.
El-Tantawy N, El-Nahas H, Salem D A, Salem N A, Hasaneen B M. Seroprevalence of Toxoplsma Gondii
and Toxocara Spp in children with cryptogenic epilepsy. AJIDM 2013; 1(5): 92-95.
Khademvatan S, Khajeddin N, Izadi S, Yousefi E. Investigation of Anti-Toxocara and Anti-Toxoplasma
antibodies in patients with schizophrenia disorder. Hindawi publishing corporation 2014; Article ID 230349.
Alipour A, Mohebali M, Tehranidoost M, Abdi Masoleh F, Keshavarz H. Toxoplasma infection in Schizophrenia patients: A comparative study with control group. Iran J Parasitol 2011; 6(2): 31-37.
Khademvatan S, Khajeddin N, Izadi S, Saki J. Study of Toxoplasma Gondii infection in patients with Bipolar disorder. J Med Sci 2013; 13(3): 215-220.
Khademvatan S, Abdizadeh R, Tavalla M. Molecular characterization of Toxocara spp. from soil of public areas in Ahvaz southwestern Iran. Acta Trop 2014;135:50-54.
Khademvatan S, Rahim F, Tavalla M, Abdizadeh R, Hashemitabar M. PCR-Based Molecular Characterization of Toxocara spp. Using Feces of Stray Cats: A Study from Southwest Iran. Plos One 2013; 8(6): e65293.
Maraghi S, Rafiei A, Hajihossein R, Sadjjadi S M. Seroprevalence of toxocariasis in hypereosinophilic individuals in Ahwaz, South-Western Iran. JHL 2011; 1-4.
Park SP, Park I, Park H, Lee S, Huh S, Kong Y&Magnaval JF. Five cases of ocular toxocariasis confirmed by serology. Korean J Parasitol 2000; 38: 267-273.
Fan C K, Hung C, Du W, Liao C W, Su K E. Seroepidemiology of ToxocaraCanis infection among
mountain aboriginal schoolchildren living in contaminated districts in Eastern Taiwan. TM & IH 2004; 9: 1312-1318.
Kim Y, Huh S, Chung Y. Seroprevalence of toxocariasis among healthy people with eosinophilia. Korean J
Parasitol 2008; 46: 29-32.
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