Loss of P16 Protein Expression and Its Association with Epstein-Barr Virus LMP-1 Expression in Hodgkin's Lymphoma

Fawzi Irshaid Irshaid--- Dept. of Biological Sciences, Faculty of Science, Al al-Bayt University, Al-Mafraq, Jordan,
Khaled Tarawneh--- . Dept. of Biological Sciences, Faculty of Science, Mutah University, Mutah, Jordan,
Aisha Alshdefat--- Mafraq Hospital of Children and Maternity, Al-Mafraq, Jordan,
Fatiha Dilmi--- Dept. of Biological Sciences, Faculty of Science, Al al-Bayt University, Al-Mafraq, Jordan,
Adnan Jaran--- Dept. of Biological Sciences, Faculty of Science, Al al-Bayt University, Al-Mafraq, Jordan,
Raji Al-Hadithi--- Dept. of Pathology, Faculty of Medicine, Jordan University of Sciences and Technology, Irbid, Jordan,
Ahad Al-Khatib--- Dept. of Pathology, Faculty of Medicine, Jordan University of Sciences and Technology, Irbid, Jordan

Abstract


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Background: Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. Thus, this study was conducted to examine the relationship between the loss of P16 and EBV LMP-1 expression in Jordanian patients diagnosed with lymphoma.

Methods: Sections were made from archival formalin-fixed and paraffin-embedded blocks from 55 patients diagnosed with lymphoma. P16 expression and LMP-1 expression were detected by immunohistochemistry using monoclonal antibodies.

Results: In Hodgkin's Lymphoma (HL), the loss of P16 was higher in LMP-1 positive cases (61%) than LMP-1 negative cases (25%; P = 0.072). Conversely, in Non-Hodgkin's Lymphoma (NHL), none of LMP-1 positive samples showed loss of P16. Furthermore, among LMP-1 HL positive cases, the loss of P16 was more frequent in male (75%) than female (33%). Also, there was a significantly higher proportion of LMP-1 positive cases showing loss of P16 in HL (11:18), compared to those in NHL (0:8, P < 0.001), confirming a difference between HL and NHL, concerning the LMP-1/P16 relationship.

Conclusion: A trend for an association between loss of P16 and LMP-1 expression was observed in HL but not NHL patients. These findings suggest that there are molecular and clinical differences in the pathogenesis and development of different subtypes of lymphoma.

Please cite this article as: Irshaid F, Tarawneh Kh, Alshdefat A, Dilmi F, Jaran A, Al-HadithiR, Al-Khatib A. Loss of P16 Protein Expression and Its Association with Epstein-Barr Virus LMP-1 Expression in Hodgkin's Lymphoma. Iran J Cancer Prev. 2013; 6(2):78-84.

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Keywords


Epstein-Barr virus infections; Immunohistochemistry; Lymphoma; Monoclonal antibodies

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