INTRODUCTION: We aimed to compare apical canal transportation of extracted teeth and two types of simulated resin blocks. MATERIALS AND METHODS: Mesiobuccal root of extracted maxillary molars, high hardness simulated resin blocks (Knoop hardness=40) and low hardness simulated resin blocks (Knoop hardness=22) were prepared with K-files using step-back technique (n=15 canals in each group). Double exposure radiographic technique was used for extracted teeth. Simulated resin blocks were stabilized and scanned before and after preparation. Pre and post-preparation pictures were superimposed and apical transportation was measured. The data were analyzed statistically using ANOVA and Tukey HSD tests. RESULTS: There was no significant difference in apical canal transportation between extracted teeth and high hardness resin blocks (P>0.05). Low hardness resin blocks showed more apical transportation than the other groups (P<0.05). CONCLUSION: Under the conditions of this study, apical canal transportation for extracted teeth and high hardness simulated resin blocks were similar.