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Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts

Maryam Javidi, Mina Zarei, Salma Omidi, Ahmad Ghorbani, Maryam Gharechahi, Maryam Shayani Rad
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Abstract

Introduction: The aim of this study was to evaluate the cytotoxicity of a new nano zinc-oxide eugenol (NZOE) sealer in comparison with AH-26 and Pulpdent root canal sealers. Methods and Materials: The L929 mouse fibroblast cells were cultivated and incubated for 24, 48 or 72 h with different dilutions (1/1, 1/2, 1/4, 1/8, 1/16 and 1/32) of culture media previously exposed to either of the test sealers naming NZOE, AH-26 or Pulpdent. At the end of incubation period, the effect of sealers on cell viability was evaluated using Mosmann’s Tetrazolium Toxicity (MTT) colorimetric assay. The data was compared using the one-way analysis of variance (ANOVA) followed by the Tukey’s post hoc test for multiple comparisons. Results: After 24, 48 or 72 h, both NZOE and Pulpdent sealers inhibited cell viability at 1/1, 1/2 and 1/8 dilutions. Within the 24 and 48 h, the AH-26 sealer reduced the cell viability at all dilutions except the 1/32 solution; however after 72 h even the 1/32 dilution was cytotoxic. Conclusion: The biocompatibility of the nano zinc-oxide eugenol sealer was comparable to Pulpdent sealer and lower than AH-26.

Keywords: Cytotoxicity; MTT Assay; Nanoparticles; Root Canal Sealer; Zinc-Oxide Eugenol
 




DOI: https://doi.org/10.22037/iej.v10i4.8403

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