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A Study on Biocompatibility of Three Endodontic Sealers: Intensity and Duration of Tissue Irritation

Camilla Christian Gomes Moura, Thais Cristina Cunha, Virgínia Oliveira Crema, Paula Dechichi, João Carlos Gabrielli Biffi
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Abstract

Introduction: Several studies have evaluated the inflammatory reaction triggered by Epiphany (EPH), a contemporary endodontic sealer. However, they used conventional parameters, which need additional analysis to better understand the reactions induced by this sealer compared to other traditional sealers. Methods and Materials: The intensity and time span of tissue irritations for three endodontic sealers were assessed by inflammatory reactions, fibrous capsule measurement and mast cell counts. Tubes containing freshly mixed EPH, AH plus (AHP) and Endofill (ENF) were subcutaneously implanted into the backs of 28 Wistar rats. The side wall of the tube was used as the control. At 14, 21, 42 and 60 days, the connective tissue surrounding the implants (n=7) was stainedfor histopathological analysis. The Friedman test was applied to compare the results. The level of significance was set at 0.05. Results: At days 14 and 21, a significant difference among the groups was observed, with the ENF showing the worst tissue response (P<0.001). ENF remained the most aggressive sealer at 42 and 60 days, compared with EPH (P<0.05). No differences were found for the fibrous capsule thicknesses among the groups in each period. The number of mast cells per field did not show difference among the sealers at 21 and 60 days. Conclusions: EPH and AHP elicited similar patterns of irritation, as demonstrated by the inflammatory scores and fibrous capsule thicknesses. ENF caused the highest degree of tissue damage. The increase in mast cell counts observed during the early and late periods shows the possibility of late hypersensitivity to the test materials.


Keywords

Biocompatible Materials; Biocompatibility Testing; Endodontics; Root Canal Filling Materials; Root Canal Obturation; Root Canal Sealants; Subcutaneous Tissue

References

Farhad AR, Hasheminia S, Razavi S, Feizi M. Histopathologic evaluation of subcutaneous tissue response to three endodontic sealers in rats. J Oral Sci. 2011;53(1):15-21.

Yamanaka Y, Shigetani Y, Yoshiba K, Yoshiba N, Okiji T. Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers. Int Endod J. 2011;44(7):669-75.

Gencoglu N, Sener G, Omurtag GZ, Tozan A, Uslu B, Arbak S, et al. Comparision of biocompatibility and cytotoxicity of two new root canal sealers. Acta Histochem. 2010;112(6):567-75.

Bouillaguet S, Wataha JC, Tay FR, Brackett MG, Lockwood PE. Initial in vitro biological response to contemporary endodontic sealers. J Endod. 2006;32(10):989-92.

de Campos-Pinto MM, de Oliveira DA, Versiani MA, Silva-Sousa YT, de Sousa-Neto MD, da Cruz Perez DE. Assessment of the biocompatibility of Epiphany root canal sealer in rat subcutaneous tissues. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105(5):e77-81.

Garcia LaF, Marques AA, Roselino LeM, Pires-de-Souza FeC, Consani S. Biocompatibility evaluation of Epiphany/Resilon root canal filling system in subcutaneous tissue of rats. J Endod. 2010;36(1):110-4.

Pameijer CH, Zmener O. Resin materials for root canal obturation. Dent Clin North Am. 2010;54(2):325-44.

Baraba A, Zelježić D, Kopjar N, Mladinić M, Anić I, Miletić I. Evaluation of cytotoxic and genotoxic effects of two resin-based root-canal sealers and their components on human leucocytes in vitro. Int Endod J. 2011;44(7):652-61.

Brackett MG, Marshall A, Lockwood PE, Lewis JB, Messer RL, Bouillaguet S, et al. Inflammatory suppression by endodontic sealers after aging 12 weeks In vitro. J Biomed Mater Res B Appl Biomater. 2009;91(2):839-44.

Onay EO, Ungor M, Ozdemir BH. In vivo evaluation of the biocompatibility of a new resin-based obturation system. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;104(3):e60-6.

Suzuki P, Souza V, Holland R, Gomes-Filho JE, Murata SS, Dezan Junior E, et al. Tissue reaction to Endométhasone sealer in root canal fillings short of or beyond the apical foramen. J Appl Oral Sci. 2011;19(5):511-6.

Silveira CM, Pinto SC, Zedebski ReA, Santos FA, Pilatti GL. Biocompatibility of four root canal sealers: a histopathological evaluation in rat subcutaneous connective tissue. Braz Dent J. 2011;22(1):21-7.

Khashaba RM, Moussa MM, Chutkan NB, Borke JL. The response of subcutaneous connective tissue to newly developed calcium phosphate-based root canal sealers. Int Endod J. 2011;44(4):342-52.

Zafalon EJ, Versiani MA, de Souza CJ, Moura CC, Dechichi P. In vivo comparison of the biocompatibility of two root canal sealers implanted into the subcutaneous connective tissue of rats. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103(5):e88-94.

Berbert FL, Sivieri-Araújo G, Ramalho LT, Pereira SA, Rodrigues DB, de Araújo MS. Quantification of fibrosis and mast cells in the tissue response of endodontic sealer irradiated by low-level laser therapy. Lasers Med Sci. 2011;26(6):741-7.

Rezzani R, Rodella L, Tartaglia GM, Paganelli C, Sapelli P, Bianchi R. Mast cells and the inflammatory response to different implanted biomaterials. Arch Histol Cytol. 2004;67(3):211-7.

Bodrumlu E, Muglali M, Sumer M, Guvenc T. The response of subcutaneous connective tissue to a new endodontic filling material. J Biomed Mater Res B Appl Biomater. 2008;84(2):463-7.

Grecca FS, Kopper PM, Santos RB, Fossati AC, Carrard VC, Acasigua GA, et al. Biocompatibility of RealSeal, its primer and AH Plus implanted in subcutaneous connective tissue of rats. J Appl Oral Sci. 2011;19(1):52-6.

Scarparo RK, Grecca FS, Fachin EV. Analysis of tissue reactions to methacrylate resin-based, epoxy resin-based, and zinc oxide-eugenol endodontic sealers. J Endod. 2009;35(2):229-32.

Khalilak Z, Vatanpour M, Javidi M, Mafi M, Afkhami F, Daneshvar F. The effect of blood on apical microleakage of Epiphany and AH26: An in vitro study. Iranian Endod Journal 2011;6(2):60-64.

Silva G, da Silva EJN, da Silva JM, Andrade-Júnior CV, Ferraz CCR. Sealing ability promoted by three different endodontic sealers. Iranian Endod Journal 2011;6(2):86-89.

Silva-Herzog D, Ramírez T, Mora J, Pozos AJ, Silva LA, Silva RA, et al. Preliminary study of the inflammatory response to subcutaneous implantation of three root canal sealers. Int Endod J. 2011;44(5):440-6.

Batista RF, Hidalgo MM, Hernandes L, Consolaro A, Velloso TR, Cuman RK, et al. Microscopic analysis of subcutaneous reactions to endodontic sealer implants in rats. J Biomed Mater Res A. 2007;81(1):171-7.

Kolokouris I, Economides N, Beltes P, Vlemmas I. In vivo comparison of the biocompatibility of two root canal sealers implanted into the subcutaneous connective tissue of rats. J Endod. 1998;24(2):82-5.

Economides N, Kotsaki-Kovatsi VP, Poulopoulos A, Kolokuris I, Rozos G, Shore R. Experimental study of the biocompatibility of four root canal sealers and their influence on the zinc and calcium content of several tissues. J Endod. 1995;21(3):122-7.

Garbuzenko E, Nagler A, Pickholtz D, Gillery P, Reich R, Maquart FX, et al. Human mast cells stimulate fibroblast proliferation, collagen synthesis and lattice contraction: a direct role for mast cells in skin fibrosis. Clin Exp Allergy. 2002;32(2):237-46.

de Lima Pereira SA, dos Santos VM, Rodrigues DB, da Cunha Castro EC, dos Reis MA, de Paula Antunes Teixeira V. Quantitative analysis of fibrosis and mast cells in the tongue of chronic chagasic patients: autopsy study. Med Mal Infect. 2007;37(4):229-33.

Jordana M. Mast cells and fibrosis--who's on first? Am J Respir Cell Mol Biol. 1993;8(1):7-8.




DOI: https://doi.org/10.22037/iej.v9i2.4962

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