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Synthesis and evaluation of multi-wall carbon nanotube–paclitaxel complex as an anti-cancer agent

Fariba Ghasemvand, Esmaeil Biazar, Sara Tavakolifard, Mohammad Khaledian, Saeid Rahmanzadeh, Daruosh Momenzadeh, Roshanak Afroosheh, Faezeh Zarkalami, Marjan Shabannezhad, Saeed Hesami Tackallou, Nilofar Massoudi, Saeed Heidari Keshel
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Abstract

Aim: The aim of this study is to design multi-walled carbon nanotubes (MWCNTs) loaded with paclitaxel (PTX) anti-cancer drug and investigate its anti-cancerous efficacy of human gastric cancer. The sidewall surfaces of pristine MWCNTs are highly hydrophobic. A major goal in carbon nanotube chemistry has been functionalization for aqueous solubility, and exploiting nano-tubes as macromolecules for chemistry, biology, and medicine application. This work establishes a novel, easy to-make formulation of a MWCNT– paclitaxel complex with high drug loading efficiency. Therefore, new opportunities in medicine  develop novel effective tumor drug delivery systems.

 Background: Carbon nanotubes (CNTs) represent a novel nano-materials applied in various fields such as drug delivery due to their unique chemical properties and high drug loading.

Patients and methods: In this study, multi-walled carbon nanotubes (MWCNTs) pre-functionalized covalently with a paclitaxel (PTX) as an anti-cancer drug and evaluated by different analyses including, scanning electron microscope (SEM), particle size analyzer and cellular analyses.

Results: A well conjugated of anti-cancer drug on the carbon nanotube surfaces was shown. This study demonstrates that the MWCN-PTX complex is a potentially useful system for delivery of anti-cancer drugs. The flow cytometry, CFU and MTT assay results have disclosed that MWCNT/PTXs might promote apoptosis in MKN-45 gastric adenocarcinoma cell line.

Conclusion: According to results, our simple method can be designed a candidate material for chemotherapy. It has presented a few bio-related applications including, their successful use as a nano-carriers for drug transport. 


Keywords

Multi-walled carbon nanotubes, Paclitaxel, Structural investigations, Gastric cancer, MKN-45 cell line.

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DOI: https://doi.org/10.22037/ghfbb.v0i0.864