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Synthesis and evaluation of multi-wall carbon nanotube–paclitaxel complex as an anti-cancer agent

Fariba Ghasemvand, Esmaeil Biazar, Sara Tavakolifard, Mohammad Khaledian, Saeid Rahmanzadeh, Daruosh Momenzadeh, Roshanak Afroosheh, Faezeh Zarkalami, Marjan Shabannezhad, Saeed Hesami Tackallou, Nilofar Massoudi, Saeed Heidari Keshel




Aim: The aim of this study is to design multi-walled carbon nanotubes (MWCNTs) loaded with paclitaxel (PTX) anti-cancer drug and investigate its anti-cancerous efficacy of human gastric cancer. The sidewall surfaces of pristine MWCNTs are highly hydrophobic. A major goal in carbon nanotube chemistry has been functionalization for aqueous solubility, and exploiting nano-tubes as macromolecules for chemistry, biology, and medicine application. This work establishes a novel, easy to-make formulation of a MWCNT– paclitaxel complex with high drug loading efficiency. Therefore, new opportunities in medicine  develop novel effective tumor drug delivery systems.

 Background: Carbon nanotubes (CNTs) represent a novel nano-materials applied in various fields such as drug delivery due to their unique chemical properties and high drug loading.

Patients and methods: In this study, multi-walled carbon nanotubes (MWCNTs) pre-functionalized covalently with a paclitaxel (PTX) as an anti-cancer drug and evaluated by different analyses including, scanning electron microscope (SEM), particle size analyzer and cellular analyses.

Results: A well conjugated of anti-cancer drug on the carbon nanotube surfaces was shown. This study demonstrates that the MWCN-PTX complex is a potentially useful system for delivery of anti-cancer drugs. The flow cytometry, CFU and MTT assay results have disclosed that MWCNT/PTXs might promote apoptosis in MKN-45 gastric adenocarcinoma cell line.

Conclusion: According to results, our simple method can be designed a candidate material for chemotherapy. It has presented a few bio-related applications including, their successful use as a nano-carriers for drug transport. 


Multi-walled carbon nanotubes, Paclitaxel, Structural investigations, Gastric cancer, MKN-45 cell line.


Biazar E, Zhang Z, Heidari S. Cellular orientation on micro-patterned biocompatible PHBV film. J. Paramed Sci. 2010; 1:74-77.

Biazar E, Heidari SK. The Healing Effect of Stem Cells Loaded in Nanofibrous Scaffolds on Full Thickness Skin Defects. J. Biomed Nanotechnol. 2013;9(9):1471-1482.

Rezaei-Tavirani M, Biazar E, AI J, Heidari S,Asefnejad A. Fabrication of collagen-coated Poly (beta-hydroxy butyrate-cobeta-hydroxyvalerate) nanofiber by chemical; and physical methods. Orient. J. Chem. 2011; 27: 385-395.

Ai J, Heidari SK, Ghorbani F, Ejazi F, Biazar E, Asefnejad A,Pourshamsian K, Montazeri M. Fabrication of coated-collagen electrospun PHBV nanofiber film by plasma method; and its cellular study. J. Nanomater. 2011; 2011: 1-8.

Biazar E,Heidari SK. Chitosan–Cross-linked nanofibrous PHBV nerve guide for rat for sciatic nerve regeneration across a defect bridge. ASAIO J. 2013; 59: 651-659.

Sahebalzamani M, Biazar E, Shahrezaei M, Hosseinkazemi H, Rahiminavaie H. Surface modification of PHBV nanofibrous mat by laminin protein and its cellular study. Int. J. Polym Mater Po. 2015;64(3):149-154.

Montazeri M, Rashidi N, Biazar E, Rad H, Sahebalzamani M, Heidari S, Majdi A. Compatibility of cardiac muscle cells on coated-gelatin electro-spun polyhydroxybutyrate- valerate nano fibrous film. Biosci. Biotech. Res. ASIA. 2011;8: 515-521.

Biazar E, Heidari S. Sahebalzamani A, Hamidi M, Ebrahimi M. The healing effect of unrestricted somatic stem cells loaded in nanofibrous Polyhydroxybutyrate-co-hydroxyvalerate scaffold on full-thickness skin defects. J Biomater Tiss Eng. 2014; 4:20-27.

Biazar E , Heidari S.A nanofibrous PHBV tube with Schwann cell as artificial nerve graft contributing to Rat sciatic nerve regeneration across a 30-mm defect bridge. Cell Commun Adhes. 2013: 20(1-2):41-49.

Biazar E , Heidari S, Pouya M. Behavioral evaluation of regenerated rat sciatic nerve by a nanofibrous PHBV conduit filled with Schwann cell as artificial nerve graft. Cell Commun Adhes. 2013;20(5):93-103.

Biazar E. Polyhydroxyalkanoates as potential biomaterials for neural tissue regeneration. Int. J. Polym Mater Po. 2014;63: 898-908.

Biazar E, Heidari S. Sahebalzamani A, Heidari M. Design of Oriented Porous PHBV Scaffold as a Neural Guide. Int. J. Polym Mater Po. 2014; 63: 753–757.

Biazar E, Heidari S. Gelatin-Modified Nanofibrous PHBV Tube as Artificial Nerve Graft for Rat Sciatic Nerve Regeneration. Int. J. Polym Mater Po. 2014;63: 330–336.

Zeinali R, Biazar E, Heidari S, Rezaei M, Asadipour K. Regeneration of Full-Thickness Skin Defects Using Umbilical Cord Blood Stem Cells Loaded into Modified Porous Scaffolds. ASAIO Journal .2014; 60:106–114.

Schultz HP. Topological Organic Chemistry. Polyhedranes and Prismanes. J Org Chem. 1965; 30:1361–1364.

Kostarelos K, Lacerda L, Pastorin G, Wu W, Wieckowski S, Luangsivilay J,Godefroy S, Pantarotto D, Briand J-P, Muller S, Prato M, Bianco A. Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type. Nat Nanotecnol. 2007; 2:108–113.

Liu Z, Chen K, Davis C, Sherlock S, Cao Q, Chen X, Dai H. Drug delivery with carbon nanotubes for in vivo cancer treatment. Cancer Res. 2008; 68:6652–6660.

Roveimiab Z, Mahdavian AR, Biazar E, Heidari S. Preparation of Magnetic Chitosan Nanocomposite Particles and Their Susceptibility for Cellular Separation Applications. Journal of Colloid Science and Biotechnology. 2012;1:82-88.

Tavakolifard S, Biazar E, Pourshamsian K, Moslemin M.H. Synthesis and evaluation of single-wall carbon nanotube-paclitaxel-folic acid conjugate as an anti-cancer targeting agent. Artif Cell Nanomed B. 2015; In press.

Tahermansouri H, Biazar E. Functionalization of carboxylated multi-wall carbon nanotubes with 3,5-diphenyl pyrazole and an investigation of their toxicity. Carbon. 2013; 63:594

Azizian J, Tahermansouri H, Biazar E, Heidari S, Chobfrosh D. Functionalization of carboxylated multiwall nanotubes with imidazole derivatives and their toxicity investigations. Int.J.Nanomed. 2010:5 907–914

Ai J, Biazar E, Jafarpour M, Montazeri M, Majdi A, Aminifard S, Zafari M, Akbari H, Rad H. Nanotoxicology - Nanoparticles Safety at Biomedical Designs. Int.J.Nanomed. 2011;6: 1117-1127.

Zhang Z, Yang X, Zhang Y. Delivery of telomerase reverse transcriptase small interfering RNA in complex with positively charged single walled nanotubes suppresses tumor growth. Clin Cancer Res. 2006;12:4933–4939.

Kam NW, O’Connell M, Wisdom JA, Dai H. Carbon nanotubes as multifunctional biological transporters and near infrared agents for selective cancer cell destruction. Proc Natl Acad Sci U S A. 2005;102:11600–11605.

Kam NW, Liu Z, Dai H. Functionalization of carbon nanotubes via cleavable disulfide bonds for efficient intracellular delivery of siRNA and potent gene silencing. J Am Chem Soc. 2005;127:12492–12493.

Gannon GI. Carbon nanotube-enhanced thermal destruction of cancer cells in a noninvasive radiofrequency field. Cancer. 2007;110:2654–2655.

Liu Z, Sun X, Nakayama-Ratchford N, Dai H. The supramolecular chemistry of organic-inorganic hybrid materials. ACS Nano. 2007;1:50–56.

Colvin, VL. The potential environmental impact of engineered nanomaterials. Nat. Biotech. 2003; 21: 1166-1170.

Warheit DB, Laurence BR, Reed KL, Roach DH, Reynolds GAM, Webb TR. Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats. Toxicol. Sci. 2004; 77: 117-125.

Bianco A, Kostarelos K, Partidos CD, Prato M. Biomedical applications of functionalized carbon nanotubes. Chem. Commun. 2005; 5: 571–577.

DOI: https://doi.org/10.22037/ghfbb.v0i0.864