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Immunological reactions by T cell and crucial genes regulation in the treated celiac disease patients

Mohammad Rostami-Nejad, Zahra Razzaghi, Somayeh Esmaeili, Sina Rezaei-Tavirani, Alireza Akbarzadeh Baghban, Reza Vafaee
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Abstract

Aim: To assess the immunological reactions and gene expression level in the celiac disease (CD) patients under gluten free diet (GFD).

Background: CD is an autoimmune disorder in genetic susceptible individuals and lifelong gluten free diet is the effective treatment method. It seems that treated patients will experience a normal life style however there are documents about some potential damages.

Methods: Gene expression profiles of treated CD patients and healthy samples were obtained from Gene Expression Omnibus (GEO) and compared to find the differentially expressed genes (DEGs). The identified DEGs were included in the network and gene ontology (GO) analysis.

Results: 10 differentially expressed genes (DEGs) including CCR2, IRF4, FASLG, CCR4, ICOS, TNFSF18, BACH2, LTF, PRM1, and PRM2 were investigated via network analysis. Seven clusters of biological processes (BP) were determined as the affected BP. The finding led to introduce CCR2, IRF4, FASLG, CCR4, and ICOS as the potential immunological markers that still active despite GFD in the treated CD patients.

Conclusion: The result of this study indicated that the immune system is already active in treated CD patients despite GFD treatment and exposure to the gluten is cause potential immunological reactions in these patients.


Keywords

Celiac disease; Gene expression; Network analysis, Immune system

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DOI: https://doi.org/10.22037/ghfbb.v13i2.1921