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Differentiation of H. pylori-negative and positive gastric cancer via regulatory network analysis

Saeid Abdi, Mona Zamanian Azodi, Mostafa Rezaei-Tavirani, Mohammadreza Razzaghi, Mohammad Hossein Heidari
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Abstract

Aim: To understand the molecular difference between H.pylori negative and positive gastric cancer, a regulatory network analysis is investigated in this research.

Background: Helicobacter pylori as the one of the most leading causes of gastric cancer is yet to be studied in terms of its molecular pathogenicity.

Methods: Cytoscape version of 3.7.2 with its applications were employed to conduct this study via corresponding algorithms.

Results: A number of 161 microRNAs were identified differentially expressed in the comparison of two groups of gastric cancer including negative and positive with H.pylori infection. CluePedia explored the regulatory network and found down-regulation dominant  by considering the linked hub genes. The potential DE miRNAs  are introduced as hsa-miR-943, hsa-miR-935, hsa-miR-367, hsa-miR-363, hsa-miR-25, and hsa-miR-196b. The most interacting hub genes for these DEmiRNAs are ADRA1A, KCNA4, SOD1, and SESN3.

Conclusion: It can be concluded that the presented microRNAs and target genes could have associations with H.pylori carcinogenesis in gastric cancer through dysregulation of some vital biological processes. However, verification analysis in this regard is required to establish these relationships.


Keywords

Helicobacter pylori, Gastric Cancer, MicroRNA, Regulatory Network, Hub

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DOI: https://doi.org/10.22037/ghfbb.v13i2.1906