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The effect of intestinal microbiota metabolites on HT29 cell line using MTT method in patients with colorectal cancer

Somayeh Jahani-Sherafat, Masoumeh Azimirad, Hajieh Ghasemian-Safaei, Hadi Ahmadi Amoli, Sharareh Moghim, Ghazal Sherkat, Esmaeil Shamsafzali, Mohammad Reza Zali
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Abstract

Aim: The aim of this study was to evaluate the effect of intestinal microbiota metabolites in colorectal cancer patients on HT29 cell line using MTT assay.

Background: Colorectal cancer is one of the most common malignant tumors, Human guts harbor abundant microbes that adjust many aspects of host physiology.  Increasing studies show that gut microbiota plays a significant role in the incidence and expansion of CRC, as a result of virulence factors, bacterial metabolites, or inflammatory pathways.

Materials and Methods: In this cross sectional study, 60 biopsy samples including 30 cancerous and 30 adjacent healthy tissues were collected from patients with CRC during 2017. Biopsy samples first cultured on Thioglycollate broth medium for 24hr and then microbiota metabolites were filtered and stored at -20 C° for further evaluation. HT29 cells were treated by microbiota metabolites in different time (3, 6, 12, 18h) and its viability was assessed by MTT assay.

Results: Treated cells with microbiota metabolites showed increased viability and proliferation in time-dependent analysis by MTT assay but there was not any significant differences between two groups.

Conclusion: It seems that microbial metabolites are able to induce proliferation and increase cell viability and therefore induction of colorectal cancer.


Keywords

Metabolites, Cell line, MTT, Colorectal cancer

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DOI: https://doi.org/10.22037/ghfbb.v12i0.1815