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Highlighted role of VEGFA in follow up of celiac disease

Sina Rezaei-Tavirani, Mohammad Rostami Nejad, Fatemeh Montazer




Aim: Evolution of gene expression change of intestine tissue in celiac patients to find a new molecular prospective of disease is the aim of this study.

Background: Celiac disease (CD) as an autoimmune disease is known as an immune reaction response to the gluten in patients. It is reported that genetic and environmental conditions are important in onset and progress of CD.

Methods: gene expression profiles of intestinal tissue in 12 celiac patients and 12 healthy controls from gene expression omnibus (GEO) were downloaded and verified by boxplot analysis. The significant and selected differentially expressed genes (DEGs) were included protein-protein interaction (PPI) network analysis. The central nodes were identified by network analyzer.

Results: The network was constructed from 161 query DEGs and 50 additional neighbors. GTF2H1, VEGFA, SUMO1, RAD51, MED21, BBP4, LEP, and MAP2K7 as potent hub nodes LRP5, RABGEF1, BCAS2, DYRK1B, AOC3, RABL2A, CRTAP, VEGFA, and SPOPL as potent bottlenecks are introduced as crucial nodes.

Conclusion: Among the crucial DEGs, Vascular endothelial growth factor A (VEGFA) was highlighted as an important biomarker candidate for follow up of celiac patients.

Keywords: Celiac disease, Biomarker, VEGFA, Follow up, Gene.

(Please cite as: Rezaei-Tavirani S, Rostami-Nejad M, Montazar F. Highlighted role of VEGFA in follow up of celiac disease. Gastroenterol Hepatol Bed Bench 2019;12(3):254-259).


Celiac disease, Biomarker, VEGFA, Follow up, Gene.


Rostami-Nejad M, Villanacci V, Mashayakhi R, Molaei M, Bassotti G, Zojaji H, et al. Celiac disease and Hp infection association in Iran. Rev Esp Enferm Dig 2009;10:850-4.

Zamanian Azodi M, Peyvandi H, Rostami-Nejad M, Safaei A, Rostami K, Vafaee R, Heidari M, et al. Protein-protein interaction network of celiac disease. Gastroenterol Hepatol Bed Bench 2016;9:268-277.

Fasano A, Catassi C. Current approaches to diagnosis and treatment of celiac disease: an evolving spectrum. Gastroenterol 2001;120:636-51.

Kemppainen T, Kröger H, Janatuinen E, Arnala I, Kosma VM, Pikkarainen P, et al. Osteoporosis in adult patients with celiac disease. Bone 1999;24:249-55.

Rostami-Nejad M, Rostami K, Cheraghipour K, Mojarad EN, Volta U, Al Dulaimi D, et al. Celiac disease increases the risk of Toxoplasma gondii infection in a large cohort of pregnant women. Am J Gastroenterol 2011;106:548.

Bardella MT, Fredella C, Prampolini L, Molteni N, Giunta AM, Bianchi PA. Body composition and dietary intakes in adult celiac disease patients consuming a strict gluten-free diet. Am J clin Nutr 2000;72:937-9.

Rezaei-Tavirani MR, Bashash D, Rostami FT, Rezaei-Tavirani SR, Nikzamir A, Rezaei-Tavirani MR, et al. Celiac disease microarray analysis based on system biology approach. Gastroenterol Hepatol Bed Bench 2018;11:216-24.

Rezaei-Tavirani M, Rezaei-Tavirani S, Mansouri V, Rostami-Nejad M, Rezaei-Tavirani M. Protein-protein interaction network analysis for a biomarker panel related to human esophageal adenocarcinoma. APJCP 2017;18:3357.

Safari-Alighiarloo N, Taghizadeh M, Rezaei-Tavirani M, Goliaei B, Peyvandi AA. Protein-protein interaction networks (PPI) and complex diseases. Gastroenterol Hepatol Bed Bench 2014;7:17.

Zamanian-Azodi M, Rezaei-Tavirani M, Rahmati-Rad S, Hasanzadeh H, Tavirani MR, Seyyedi SS. Protein-Protein Interaction Network could reveal the relationship between the breast and colon cancer. Gastroenterol Hepatol Bed Bench 2015;8:215.

Ideker T, Sharan R. Protein networks in disease. Genome Res 2008;18:644-52.

Goñi J, Esteban FJ, De Mendizábal NV, Sepulcre J, Ardanza-Trevijano S, Agirrezabal I, et al. A computational analysis of protein-protein interaction networks in neurodegenerative diseases. BMC System Biol 2008;2:52.

Brysbaert G, Mauri T, Lensink MF. Comparing protein structures with RINspector automation in Cytoscape. F1000 Res 2018;7.

Rezaei-Tavirani M, Rezaei-Tavirani M, Mansouri V, Mahdavi SM, Valizadeh R, Rostami-Nejad M, et al. Introducing crucial protein panel of gastric adenocarcinoma disease. Gastroenterol Hepatol Bed Bench 2017;10:21.

Li BQ, Huang T, Liu L, Cai YD, Chou KC. Identification of colorectal cancer related genes with mRMR and shortest path in protein-protein interaction network. PLoS One 2012;7:e33393.

Shibuya M. Vascular endothelial growth factor (VEGF) and its receptor (VEGFR) signaling in angiogenesis: a crucial target for anti-and pro-angiogenic therapies. Genes Cancer 2011;2:1097-105.

Massironi S, Branchi F, Rossi RE, Fraquelli M, Elli L, Bardella MT, et al. Hepatic hemangioma in celiac patients: data from a large consecutive series. Gastroenterol Res Pract 2015;2015.

Taha Y, Raab Y, Larsson A, Carlson M, Lööf L, Gerdin B, et al. Vascular endothelial growth factor (VEGF)—a possible mediator of inflammation and mucosal permeability in patients with collagenous colitis. Dig Dis Sci 2004;49:109-15.

DOI: https://doi.org/10.22037/ghfbb.v12i3.1724