Molecular diagnosis of strongyloidiasis in a population of an endemic area through nested-PCR

Meysam Sharifdini, Amir Keyhani, Mohammadreza Eshraghian, Eshrat BeigomKia





elderly, molecular diagnosis, nested-PCR, Strongyloides stercoralis


Olsen, A.; van Lieshout, L.; Marti, H.; Polderman, T.; Polman, K.; Steinmann, P.; Stothard, R.; Thybo, S.; Verweij, J.J.; Magnussen, P. Strongyloidiasis--the most neglected of the neglected tropical diseases? Trans R Soc Trop Med Hyg 2009; 103: 967-72.

Segarra-Newnham, M. Manifestations, diagnosis, and treatment of Strongyloides stercoralis infection. Ann Pharmacother 2007;41; 1992-2001.

Grove, D.I. Human strongyloidiasis. Adv Parasitol 1996, 38, 251-309.

Keiser, P.B.; Nutman, T.B. Strongyloides stercoralis in the Immunocompromised Population. Clin Microbiol Rev 2004; 17: 208-17.

Siddiqui, A.A.; Berk, S.L. Diagnosis of Strongyloides stercoralis infection. Clin Infect Dis 2001; 33: 1040-7.

Arakaki T, Iwanaga M, Kinjo F, Saito A, Asato R, Ikeshiro T. Efficacy of agar-plate culture in detection of Strongyloides stercoralis infection. J Parasitol 1990;76:425-28.

Kia EB, Mahmoudi M, Zahabiun F, Meamar AR. An evaluation on the efficacy of agar plate culture for detection of Strongyloides stercoralis. Iran J Parasitol 2007;2:29-34.

Marcos LA, Terashima A, Dupont HL, Gotuzzo E. Strongyloides hyperinfection syndrome: an emerging global infectious disease. Trans R Soc Trop Med Hyg 2008;102:314-18.

Verweij JJ, Canales M, Polman K, Ziem J, Brienen EA, Polderman AM, van Lieshout L.. Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR. Trans R Soc Trop Med Hyg 2009;103:342-6.

ten Hove RJ, Verweij JJ, Vereecken K, Polman K, Dieye L, van Lieshout L. Multiplex real-time PCR for the detection and quantification of Schistosoma mansoni and S. haematobium infection in stool samples collected in northern Senegal. Trans R Soc Trop Med Hyg 2008;102:179-85.

Verweij JJ. Application of PCR-based methods for diagnosis of intestinal parasitic infections in the clinical laboratory. Parasitology 2014;141:1863-72.

Sharifdini M, Mirhendi H, Ashrafi K, Hosseini M, Mohebali M, Khodadadi H, Kia EB. Comparison of nested polymerase chain reaction and real-time polymerase chain reaction with parasitological methods for detection of Strongyloides stercoralis in human fecal samples. Am J Trop Med Hyg 2015;93:1285-91.

Dacal E, Saugar JM, Soler T, Azcarate JM, Jimenez MS, Merino FJ, Rodríguez E. Parasitological versus molecular diagnosis of strongyloidiasis in serial stool samples: how many? J Helminthol 2017:1-5.

Arfa F. Medical Helminthology: Danesh Pazhooh Publications; 2017.

Sharifdini M, Kia EB, Ashrafi K, Hosseini M, Mirhendi H, Mohebali M, Kamranrashani B. An analysis of clinical characteristics of Strongyloides stercoralis in 70 indigenous patients in Iran. Iran J Parasitol 2014;9:155-62.

Zarasvandi A, Carranza EJM, Moore F, Rastmanesh F. Spatio-temporal occurrences and mineralogical-geochemical characteristics of airborne dusts in Khuzestan province (southwestern Iran). J Geochem Explor 2011;111:138–51.

Koga K, Kasuya S, Ohtomo H. How effective is the agar plate method for Strongyloides stercoralis? J Parasitol. 1992;78:155-6.

Inatomi S, Kamo H, Otsuru M, Suzuki T, Yoshida Y. Ova and larvae of the common helminthes of man. In: Yamaguchi T, Editor. A colour atlas of clinical parasitology. Tokyo: Wolf Medical Publication; 1981.

Johnston FH, Morris PS, Speare R, McCarthy J, Currie B, Ewald D, Page W, Dempsey K. Strongyloidiasis: a review of the evidence for Australian practitioners. Aust J Rural Health 2005;13:247-54.

Knopp S, Salim N, Schindler T, Karagiannis Voules DA, Rothen J, Lweno O, Mohammed AS, Singo R, Benninghoff M, Nsojo AA, Genton B, Daubenberger C. Diagnostic accuracy of Kato-Katz, FLOTAC, Baermann, and PCR methods for the detection of light-intensity hookworm and Strongyloides stercoralis infections in Tanzania. Am J Trop Med Hyg 2014; 90: 35–545.

Repetto SA, Alba Soto CD, Cazorla SI, Tayeldin ML, Cuello S, Lasala MB, Tekiel VS, González Cappa SM. An improved DNA isolation technique for PCR detection of Strongyloides stercoralis in stool samples. Acta Trop. 2013;126:110-14.

Schar F, Odermatt P, Khieu V, Panning M, Duong S, Muth S, Marti H, Kramme S. Evaluation of real-time PCR for Strongyloides stercoralis and hookworm as diagnostic tool in asymptomatic schoolchildren in Cambodia. Acta Trop 2013;126:89-92.

Sultana Y, Jeoffreys N, Watts MR, Gilbert GL, Lee R. Real-time polymerase chain reaction for detection of Strongyloides stercoralis in stool. Am J Trop Med Hyg 2013;88:1048-51.

Ashrafi K, Tahbaz A, Rahmati B. Strongyloides stercoralis: the most prevalent parasitic cause of eosinophilia in Gilan Province, northern Iran. Iran J Parasitol 2010;5:40-7.

Shokri A, Sarasiabi KS, Teshnizi SH, Mahmoodi H. Prevalence of Strongyloides stercoralis and other intestinal parasitic infections among mentally retarded residents in central institution of southern Iran. Asian Pac J Trop Biomed 2012;2:88-91.

Ahmadi M, Kia EB, Rezaeian M, Hosseini M, Kamranrashani B, Tarighi F. Prevalence of Strongyloides stercoralis and other intestinal parasites in rehabilitation centers in Mazandaran province, northern Iran. J Mazandaran Univ Med Sci 2015;25:1-7.

Mowlavi GR, MirAhmadi H, Rezaeian M, Kia EB, EbrahimiDaryani N, Rokni MB, Golestan B, Shafiei R, Fereshtehnejad SM, Keramati MR. Prevalence of intestinal parasites in tribal parts of Khuzestan Province during 2005-07. Govaresh. 2008;12:219-28.

Khieu V, Schar F, Forrer A, Hattendorf J, Marti H, Duong S, Vounatsou P, Muth S, Odermatt P.High prevalence and spatial distribution of Strongyloides stercoralis in rural Cambodia. PLoS Negl Trop Dis 2014;8:e2854.

Chordia P, Christopher S, Abraham OC, Muliyil J, Kang G, Ajjampur S. Risk factors for acquiring Strongyloides stercoralis infection among patients attending a tertiary hospital in south India. Indian J Med Microbiol 2011;29: 147-51.


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PISSN: 2008-2258

EISSN: 2008-4234


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