Exploring conserved mRNA-miRNA interactions in colon and lung cancers

fereshteh izadi, Mona Zamanian-azodi, Vahid Mansouri

Abstract


1707

MicroRNAs (miRNAs) as small and endogenous noncoding RNAs regulate gene expression by repressing mRNA translation or decreasing stability of mRNAs and has proven pivotal roles in different types of cancers. Accumulating evidences have indicated the role of miRNAs in a wide range of biological processes from oncogenesis and tumor suppressors to the participation in tumor progression. Colon and lung cancers are frequently encountered challenging types of cancers therefore exploring trade-off among underlying biological units such as miRNA with mRNAs probably will lead to the identification of promising biomarkers involved in these malignancies.

Colon cancer and lung cancer expression data were downloaded from Firehose and TCGA databases and varied genes extracted by DCGL software were subjected to build two gene regulatory networks by parmigene R package. Afterward a network-driven integrative analysis was performed to exploring prognosticates genes, miRNAs and underlying pathways.

Total of 192 differentially expressed miRNAs and their target genes within gene regulatory networks were derived by ARACNE algorithm. BTF3, TP53, MYC, CALR, NEM2, miR-29b-3p and miR-145 were identified as bottleneck nodes and enriched via biological gene ontology (GO) terms and pathways chiefly in biosynthesis and signaling pathways by further screening. The main goal of this analysis was the prioritization of co-expressed genes and miRNAs that thought to have important influences in the pathogenesis of colon and lung cancers.




DOI: http://dx.doi.org/10.22037/ghfbb.v0i0.1198

Creative Commons License
GHFBB by Gastroenterology and Liver Diseases Research Institute is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

PISSN: 2008-2258

EISSN: 2008-4234


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