Cryptosporidium species subtypes and associated clinical manifestation in Indian patients

Shehla Khalil, Bijay Ranjan Mirdha, Ashutosh Panda, Yogita Singh, Jaishree Paul, Govind Makharia



Aim: Present hospital based study was carried out at our tertiary care centre and the aim of the study was to gain insights into epidemiology of cryptosporidiosis with in northern part of the country.

Background: Cryptosporidium spp. are an important causative agent of diarrhea. Cryptosporidium hominis and Cryptosporidium parvum are the two main species causing majority of human infections.

Methods: Microscopic examinations as well as molecular methods were used for detection and identification of Cryptosporidium spp. and subtyping of Cryptosporidium hominis (C. hominis) and C. parvum.

Results: 41 patients were positive for Cryptosporidium spp. by microscopy and 43 were positive by PCR assay. Of the PCR positive samples, 70% (n=30) were C. hominis, 21% (n=9) were C. parvum, 7% (n=3) were C. felis and 2% (n=1) was C. viatorum. Using this method, 16 subtypes were identified belonging to 8 subtype families. The frequency of subtype families were Ia (13%, 5/39), Ib (15%, 6/39), Id (18%, 7/39), Ie (30%, 12/39), IIa (5%, 2/39), IIc (8%, 3/39), IId (8%, 3/39) and IIe (3%, 1/39).

Conclusion: These results strongly suggest that transmission of cryptosporidiosis is mostly anthroponotic in origin in the patients attending our tertiary care centre. We expect that our molecular epidemiological data will make a significant contribution to unravel the actual epidemiological situation of cryptosporidiosis in this part of the country.


Cryptosporidium, hospital, subtyping, gp60, phylogeny


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