Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
Gastroenterology and Hepatology from Bed to Bench,
Vol. 10 No. 2 (2017),
3 Farvardin 2017
,
Page 125-130.
https://doi.org/10.22037/ghfbb.v0i0.1003
Abstract
Aim: This work aims to isolate and perform comparative studies of a phages active against a Salmonella enteritidis strain from Iran. Also, suitable phage candidates for therapy of mice will be selected.
Background: Bacteriophage is of particular interest as a biocontrol agent in the prevention of food-borne illnesses. In recent years tend to use bacteriophages to control pathogenic bacteria has increased. A bacteriophage is considered to be a potent antibiotic alternative for treating bacterial infections.
Methods: the specific phages against Salmonella Enteritidis was isolated and candidates for therapy of mice will be selected. Mouses divided into the six specific groups. Groups of mice were as follows: A: Bacteri (control) B: Bacteri+ bacteriophage (Simultaneous), C: Bacteri + bacteriophage Four days later, D: Bacteriophage + bacteri four days later E: Bacteri+ Ciprofloxacin (Simultaneous) F: Bacteri+ ciprofloxacin+ bacteriophage (Simultaneous).
Results: In this study, a lytic bacteriophage is isolated and it shows that phage has a head size of 46 nm and without a tail, by using an electron microscope. Oral administration of a single dose of 2 × 109 PFU/mouse bacteriophage enable to protect mouse against salmonellosis and it causes treatment of salmonellosis in mice.
Conclusion: The use of this phage compared to ciprofloxacin shows that in addition of the treatment of mouse, it also prevents weight loss.
- Bacteriophage
- Salmonella enteritidis
- Isolation
How to Cite
References
Capparelli R, Nocerino N, Iannaccone M, Ercolini D, Parlato M, Chiara M, Iannelli D: Bacteriophage therapy of Salmonella enterica: a fresh appraisal of bacteriophage therapy. J Infect Dis 2010; 201(1),52-61
Crump JA, Medalla FM, Joyce KW, Krueger AL, Hoekstra RM, Whichard JM, Barzilay EJ: Antimicrobial resistance among invasive nontyphoidal Salmonella enterica isolates in the United States: National Antimicrobial Resistance Monitoring System, 1996 to 2007. Antimicrob Agents Chemother 2011; 55(3),1148-54
Ercis S, Erdem B, Hasçelik G, Gür D: Nalidixic acid resistance in Salmonella strains with decreased susceptibility to ciprofloxacin isolated from humans in Turkey. Jpn J Infect Dis 2006; 59(2),117-9
Segovia C, Vasquez I, Maracaja-Coutinho V, Robeson J, Santander J:Complete Genome Sequence of Salmonella enterica Serovar Enteritidis Bacteriophage f18SE, Isolated in Chile. Genome Announc 2015; 3(5)
Pouillot F, Chomton M, Blois H, Courroux C, Noelig J, Bidet P, Bingen E, Bonacorsi S:Efficacy of bacteriophage therapy in experimental sepsis and meningitis caused by acloneO25b:H4-ST131Escherichia coli strain producing CTXM-15,” Antimicrob Agents Chemother 2012; 56(7), 3568–3575
Biswas B, Adhya S, Washart P, Paul B, Trostel AN, Powell B, Carlton R, Merril CR:Bacteriophage therapy rescues mice bacteremic from a clinical isolate of vancomycin resistant Enterococcus faecium,” Infection and Immunity 2002; 70(1),204–210
Gu J, Liu X, Li Y, Han W, Lei L, Yang Y, Zhao H, Gao Y, Song J, Lu R, Sun C, Feng X: A method for generation phage cocktail with great therapeutic potential, PLoSONE 2012 ;7(3)
Kutter E, De Vos D, Gvasalia G, Alavidze Z, Gogokhia L, Kuhl S, Abedon ST: Phagetherapy in clinical practice :treatment of human infections, Current Pharmaceutical Biotechnology 2010; 11(1), 69–86
Brüssow H: What is needed for phage therapy to become a reality in Western medicine? Virology,2012 ;434(2), 138– 142
Chan BK , Abedon ST, Loc-Carrillo C: Phage cocktails and the future of phage therapy, Future Microbiology 2013; 8(6), 769–783
Cao F, Wang X, Wang L, Li Z, Che J, Wang L, Li X, Cao Z, Zhang J, Jin L, Xu Y: Evaluation of the efficacy of a bacteriophage in the treatment of pneumonia induced by multidrug resistance Klebsiella pneumoniae in mice. Biomed Res Int. (2015 )
Dunkley, K.D.; Callaway, T.R.; Chalova, V.I.; McReynolds, J.L.; Hume, M.E.; Dunkley, C.S.; Kubena, L.F.; Nisbet, D.J.; Ricke, S.C: Foodborne Salmonella ecology in the avian gastrointestinal tract. Anaerobe 15, 26–35(2009)
Galarce, N.E.; Bravo, J.L.; Robeson, J.P.; Borie, C.F. Bacteriophage cocktail reduces Salmonella enterica serovar Enteritidis counts in raw and smoked salmon tissues. Rev. Argent. Microbiol 2014; 46, 333–337
Kutateladze, M.; Adamia, R. Phage therapy experience at the Eliava Institute. Med. Mal. Infect 2008; 38, 426–430
Harper DR , Enright MC. Bacteriophages for the treatment of Pseudomonas aeruginosa infections, J Appl Microbiol 2011
Morello E, Saussereau E, Maura D, Huerre M, Touqui L, Debarbieux L:Pulmonary bacteriophage therapy on Pseudomonas aeruginosa cystic fibrosis strains: First steps towards treatment and prevention PLoS One 2011; 15(6)
Bordon J , Aliberti S, Fernandez-Botran R, Uriarte SM, Rane MJ, Duvvuri P, Peyrani P, Morlacchi LC, Blasi F, Ramirez JA:Understanding the roles of cytokines and neutrophil activity and neutrophil apoptosis in the protective versus deleterious inflammatory response in pneumonia, Int J Infect Dis 2013; 76-83
Kang HW , Kim JW, Jung TS, Woo GJ. wksl3, a New biocontrol agent for Salmonella enterica serovars enteritidis and typhimurium in foods: characterization, application, sequence analysis, and oral acute toxicity study. Appl Environ Microbiol 2013 79(6),1956-68
Gilles B, Armando N, Shafiqul A. S, Anne-C. Pittet , Firdausi Q , Shamima S, Alejandro C, Kaisar A. Talukder , Gloria R andHarald B . Coverage of diarrhoea-associated Escherichia coli isolates from different origins with two types of phage cocktails. Microbial Biotechnology 2014; 7(2), 165–176
Firoozeh F, Zahraei-Salehi T, Shahcheraghi F, Karimi V, Aslani MM: Characterization of class I integrons among Salmonella enterica serovar Enteritidis isolated from humans and poultry. FEMS Immunol Med Microbiol 2012; 64(2):237-43
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