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Helicobacter pylori CagA induced interleukin-8 secretion in gastric epithelial cells

Zeinab Fazeli, Masoud Alebouyeh, Mostafa Rezaei Tavirani, Masoumeh Azimirad, Abbas Yadegar
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Abstract

Aim: Since, contradictory data have been reported about the effect of diverse variants of H. pylori virulence factors on IL-8 induction, we aimed to analyze the effect of this diversity on levels of IL-8 secretion in AGS cell line.

Background: Helicobacter pylori colonizes the human stomach and induces the activation of inflammatory cytokines, including interleukin (IL)-8, in the gastric mucosa. This induction promotes neutrophil and monocyte recruitment that causes gastric tissue damage.

Methods: To determine whether different strains of H. pylori and their CagA variants have possible roles on IL-8 induction, polarized

AGS cell line was infected with CagA+ H. pylori strains carrying different EPIYA motifs (ABCCC and ABC) and CagA- strain for 24 hours. Difference in stimulation of IL-8 was measured by ELISA.

Results: IL-8 secretion was elevated in the treated cells with CagA encoding strains compared with the negative one. Furthermore, a noticeably increased level of IL-8 induction was measured by the CagA-EPIYA type ABCCC encoding strain in compare to that carried EPIYA type ABC

Conclusion: Results of this study provide new evidence about different effects of H. pylori strains and possible roles of their CagA variants on IL-8 induction. It seems that not only carriage of cagA and its expression, but also diversity in EPIYA motif be involved in IL-8 induction in the gastric epithelial cells.


Keywords

Helicobacter pylori; cytotoxin-associated gene A; interleukin-8.

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DOI: https://doi.org/10.22037/ghfbb.v0i0.1001