The correlation between quorum sensing genes (pqsR and lasR) in antibiotic resistance of Pseudomonas aeruginosa isolated from burned patients
Archives of Medical Laboratory Sciences,
Vol. 4 No. 4 (2018),
16 February 2020
https://doi.org/10.22037/amls.v4i4.28281
Abstract
Background: Pseudomonas aeruginosa, a gram-negative bacillus, and opportunistic pathogen, is an important microorganism involved with infections in burn patients worldwide. It produces biofilms by Quorum-sensing signals and makes an antibiotic resistance.
Materials and Methods: From April to September 2018, 100 samples of burn injuries were collected from the Central Hospital of Shahid Beheshti in Kashan. The samples were identified in terms of biochemical and phenotypic tests and a definitive diagnosis of P.aeruginosa species was examined based on a toxA gene by the PCR method. The positive samples were analyzed for antibiotics of amikacin, ciprofloxacin, norfloxacin, gentamicin, cefepime, aztreonam, meropenem, ceftazidime, colistin, and piperacillin-tazobactam. Then, samples were analyzed for lasR and pqsR (quorum-sensing genes) by PCR.
Results: We verified eighty-five (85%) isolates as P. aeruginosa. According to antibiograms, 92% of the isolates were considered as multidrug-resistant (MDR), of which 85.5% were extensively drug-resistant (XDR) and none of the pan drug resistance (PDR). Also, in MDR isolates, there was one nonsense mutation. In XDR samples, two isolates had a missense mutation and nonsense mutation was seen in one strain.
Conclusion: The results of our study show that with increasing resistance rates, more mutations occur in lasR and pqsR genes and the possibly can play a key role in antibiotic resistance. Given the mutations found in the quorum sensing genes, it can be concluded that these genes are mutagenic genes that will be effective in changing bacterial behavior and adaptability to environmental conditions.
- Quorum sensing
- Antibiotic resistance
- Pseudomonas aeruginosa
- Burned patients
How to Cite
References
Labarrere CA, Woods JR, Hardin JW, Campana GL, Ortiz MA, Jaeger BR, Reichart B, Bonnin JM, Currin A, Cosgrove S, Pitts DE. Early prediction of cardiac allograft vasculopathy and heart transplant failure. American Journal of Transplantation. 2011 Mar;11(3):528-35.
Fisher JF, Mobashery S. The sentinel role of peptidoglycan recycling in the β-lactam resistance of the Gram-negative Enterobacteriaceae and Pseudomonas aeruginosa. Bioorganic chemistry. 2014 Oct 1;56:41-8.
Bonomo RA, Szabo D. Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa. Clinical infectious diseases. 2006 Sep 1;43(Supplement_2):S49-56.
Bouffartigues E, Moscoso JA, Duchesne R, Rosay T, Fito-Boncompte L, Gicquel G, Maillot O, Bénard M, Bazire A, Brenner-Weiss G, Lesouhaitier O. The absence of the Pseudomonas aeruginosa OprF protein leads to increased biofilm formation through variation in c-di-GMP level. Frontiers in microbiology. 2015 Jun 23;6:630.
Breidenstein EB, de la Fuente-Núñez C, Hancock RE. Pseudomonas aeruginosa: all roads lead to resistance. Trends in microbiology. 2011 Aug 1;19(8):419-26.
Cabot G, Zamorano L, Moyà B, Juan C, Navas A, Blázquez J, Oliver A. Evolution of Pseudomonas aeruginosa antimicrobial resistance and fitness under low and high mutation rates. Antimicrobial agents and chemotherapy. 2016 Mar 1;60(3):1767-78.
Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson‐Liljequist B, Paterson DL. Multidrug‐resistant, extensively drug‐resistant and pandrug‐resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection. 2012 Mar;18(3):268-81.
Schuster M, Joseph Sexton D, Diggle SP, Peter Greenberg E. Acyl-homoserine lactone quorum sensing: from evolution to application. Annual review of microbiology. 2013 Sep 8;67:43-63.
Schafhauser J, Lepine F, McKay G, Ahlgren HG, Khakimova M, Nguyen D. The stringent response modulates 4-hydroxy-2-alkylquinoline biosynthesis and quorum-sensing hierarchy in Pseudomonas aeruginosa. Journal of bacteriology. 2014 May 1;196(9):1641-50.
Tan SY, Chua SL, Chen Y, Rice SA, Kjelleberg S, Nielsen TE, Yang L, Givskov M. Identification of five structurally unrelated quorum-sensing inhibitors of Pseudomonas aeruginosa from a natural-derivative database. Antimicrobial agents and chemotherapy. 2013 Nov 1;57(11):5629-41.
Jensen V, Löns D, Zaoui C, Bredenbruch F, Meissner A, Dieterich G, Münch R, Häussler S. RhlR expression in Pseudomonas aeruginosa is modulated by the Pseudomonas quinolone signal via PhoB-dependent and-independent pathways. Journal of bacteriology. 2006 Dec 15;188(24):8601-6.
Klare W, Das T, Ibugo A, Buckle E, Manefield M, Manos J. Glutathione-disrupted biofilms of clinical Pseudomonas aeruginosa strains exhibit an enhanced antibiotic effect and a novel biofilm transcriptome. Antimicrobial agents and chemotherapy. 2016 Aug 1;60(8):4539-51.
Rasamiravaka T, Labtani Q, Duez P, El Jaziri M. The formation of biofilms by Pseudomonas aeruginosa: a review of the natural and synthetic compounds interfering with control mechanisms. BioMed research international. 2015;2015.
Jones AM, Govan JR, Doherty CJ, Dodd ME, Isalska BJ, Stanbridge TN, Webb AK. Identification of airborne dissemination of epidemic multiresistant strains of Pseudomonas aeruginosa at a CF centre during a cross infection outbreak. Thorax. 2003 Jun 1;58(6):525-7.
CLSI. M100 Performance Standards for Antimicrobial.
Yan JJ, Hsueh PR, Lu JJ, Chang FY, Ko WC, Wu JJ. Characterization of acquired β-lactamases and their genetic support in multidrug-resistant Pseudomonas aeruginosa isolates in Taiwan: the prevalence of unusual integrons. Journal of antimicrobial chemotherapy. 2006 Jul 1;58(3):530-6.
J Wolter D, D Lister P. Mechanisms of β-lactam resistance among Pseudomonas aeruginosa. Current pharmaceutical design. 2013 Jan 1;19(2):209-22.
Tian ZX, Yi XX, Cho A, O’Gara F, Wang YP. CpxR activates MexAB-OprM efflux pump expression and enhances antibiotic resistance in both laboratory and clinical nalB-type isolates of Pseudomonas aeruginosa. PLoS pathogens. 2016 Oct 13;12(10):e1005932.
Oie S, Fukui Y, Yamamoto M, Masuda Y, Kamiya A. In vitro antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-β-lactamase-producing Pseudomonas aeruginosa. BMC infectious diseases. 2009 Dec;9(1):123.
Memar MY, Pormehrali R, Alizadeh N, Ghotaslou R, Bannazadeh Baghi H. Colistin, an option for treatment of multiple drug resistant Pseudomonas aeruginosa. Physiology and Pharmacology. 2016 May 10;20(2):130-6.
Cao H, Krishnan G, Goumnerov B, Tsongalis J, Tompkins R, Rahme LG. A quorum sensing-associated virulence gene of Pseudomonas aeruginosa encodes a LysR-like transcription regulator with a unique self-regulatory mechanism. Proceedings of the National Academy of Sciences. 2001 Dec 4;98(25):14613-8.
Chan KG, Liu YC, Chang CY. Inhibiting N-acyl-homoserine lactone synthesis and quenching Pseudomonas quinolone quorum sensing to attenuate virulence. Frontiers in microbiology. 2015 Oct 19;6:1173.
Déziel E, Gopalan S, Tampakaki AP, Lépine F, Padfield KE, Saucier M, Xiao G, Rahme LG. The contribution of MvfR to Pseudomonas aeruginosa pathogenesis and quorum sensing circuitry regulation: multiple quorum sensing‐regulated genes are modulated without affecting lasRI, rhlRI or the production of N‐acyl‐l‐homoserine lactones. Molecular microbiology. 2005 Feb;55(4):998-1014.
Schuster M, Joseph Sexton D, Diggle SP, Peter Greenberg E. Acyl-homoserine lactone quorum sensing: from evolution to application. Annual review of microbiology. 2013 Sep 8;67:43-63.
Wu G, Yan S. Can Biofilm Be Reversed Through Quorum Sensing in Pseudomonas aeruginosa?. Frontiers in microbiology. 2019;10:1582.
Rumbaugh KP, Griswold JA, Iglewski BH, Hamood AN. Contribution of quorum sensing to the virulence ofpseudomonas aeruginosa in burn wound infections. Infection and immunity. 1999 Nov 1;67(11):5854-62.
Golpasha ID, Mousavi SF, Owlia P, Siadat SD, Irani S. Immunization with 3-oxododecanoyl-L-homoserine lactone-r-PcrV conjugate enhances survival of mice against lethal burn infections caused by Pseudomonas aeruginosa. Bosnian journal of basic medical sciences. 2015 May;15(2):15.
Coetzee E, Rode H, Kahn D. Pseudomonas aeruginosa burn wound infection in a dedicated paediatric burns unit. South African Journal of Surgery. 2013 Jan;51(2):50-3.
Jahromi SI, Mardaneh J, Sharifi A, Pezeshkpour V, Behzad-Behbahani A, Seyyedi N, Dehbidi GR, Manzouri L, Pourmasoudi M, Khoramrooz SS, Khosravani SA. Occurrence of a multidrug resistant pseudomonas aeruginosa strains in hospitalized patients in southwest of Iran: Characterization of resistance trends and virulence determinants. Jundishapur Journal of Microbiology. 2018 May;11(4).
Japoni A, Farshad S, Alborzi A. Pseudomonas aeruginosa: Burn infection, treatment and antibacterial resistance. Iranian Red Crescent Medical Journal. 2009 Apr;11(3):244.
Wilder CN, Diggle SP, Schuster M. Cooperation and cheating in Pseudomonas aeruginosa: the roles of the las, rhl and pqs quorum-sensing systems. The ISME journal. 2011 Aug;5(8):1332.
D'Argenio DA, Wu M, Hoffman LR, Kulasekara HD, Déziel E, Smith EE, Nguyen H, Ernst RK, Larson Freeman TJ, Spencer DH, Brittnacher M. Growth phenotypes of Pseudomonas aeruginosa lasR mutants adapted to the airways of cystic fibrosis patients. Molecular microbiology. 2007 Apr;64(2):512-33.
López-Causapé C, Sommer LM, Cabot G, Rubio R, Ocampo-Sosa AA, Johansen HK, Figuerola J, Cantón R, Kidd TJ, Molin S, Oliver A. Evolution of the Pseudomonas aeruginosa mutational resistome in an international cystic fibrosis clone. Scientific reports. 2017 Jul 17;7(1):5555.
Lima JL, Alves LR, Jacomé PR, Neto B, Pacífico J, Maciel MA, Morais MM. Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing. Brazilian Journal of Infectious Diseases. 2018 Mar;22(2):129-36.
Cao H, Lai Y, Bougouffa S, Xu Z, Yan A. Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853. BMC genomics. 2017 Dec;18(1):459.
Zhang Z, Miteva MA, Wang L, Alexov E. Analyzing effects of naturally occurring missense mutations. Computational and mathematical methods in medicine. 2012;2012.
Zhou H, Gao M, Skolnick J. ENTPRISE-X: Predicting disease-associated frameshift and nonsense mutations. PloS one. 2018 May 3;13(5):e0196849.
- Abstract Viewed: 153 times
- PDF Downloaded: 84 times