Autophagy knock down: as a booster for the replication of viruses in cell culture
Archives of Medical Laboratory Sciences,
Vol. 2 No. 2 (2016),
16 September 2016
https://doi.org/10.22037/amls.v2i2.14931
Abstract
Background: Autophagy suppression recently has been known to have a remarkable effect for cellular adjustment and viability in the final stages of cancer. On the other hand, autophagy has the potential effect in preventing many viruses from replication. Beclin1 is the most substantial constituent in autophagy apparatus regulation. This study was intended to investigate the beclin1 siRNA knockdown effect on the extent of activity of the oncolytic vesicular stomatitis virus (VSV) as a model in cell culture.
Materials and Methods: In the current study, the cancer cell line , HeLa (cervical squamous cancer cell line ) was infected by VSV, followed by beclin1 siRNA vector transfection. The potential change in the expressions of gene beclin1 in transfected cells, as well as untransfected ones were examined by real time PCR, and also the titer of viruses was compared in cells with and without transfection.
Results: The results revealed that the amount of putative gene beclin1 expression in HeLa cells decreased greatly due to siRNA suppressive impact, and also the sensitivity of the cells to VSV oncolytic effect increased upon decrease in beclin1gene expression.
Conclusion: It seems that autophagy suppression by using siRNA with VSV is a substantial aid for increase in virus titer in cancer cell lines.
- Autophagy
- Vesicular Stomatitis Virus
- Beclin1
- Virus therapy
How to Cite
References
Taneike M, Yamaguchi O, Nakai A, Hikoso S, Takeda T, Mizote I, et al. Inhibition of autophagy in the heart induces age-related cardiomyopathy. Autophagy. 2010;6(5):600-6.
White E, Karp C, Strohecker AM, Guo Y, Mathew R. Role of autophagy in suppression of inflammation and cancer. Current opinion in cell biology. 2010;22(2):212-7.
Choi KS. Autophagy and cancer. Experimental & molecular medicine. 2012;44(2):109-20.
Mah LY, Ryan KM. Autophagy and cancer. Cold Spring Harbor perspectives in biology. 2012;4(1):a008821.
Kang R, Zeh H, Lotze M, Tang D. The Beclin 1 network regulates autophagy and apoptosis. Cell Death & Differentiation. 2011;18(4):571-80.
Shelly S, Lukinova N, Bambina S, Berman A, Cherry S.Autophagy is an essential component of Drosophila immunity against vesicular stomatitis virus. Immunity. 2009;30(4):588-98.
Wang HY, Yang GF, Huang YH, Huang QW, Gao J, Zhao XD, et al. Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma. Oncology letters. 2014;8(4):1492-8.
Jing K, Lim K. Why is autophagy important in human diseases? Experimental & molecular medicine. 2012;44(2):69-72.
Siegel R, DeSantis C, Virgo K, Stein K, Mariotto A, Smith T, et al. Cancer treatment and survivorship statistics, 2012. CA: a cancer journal for clinicians. 2012;62(4):220-41.
Wong HH, Lemoine NR, Wang Y. Oncolytic viruses for cancer therapy: overcoming the obstacles. Viruses. 2010;2(1):78-106.
Patel MR, Kratzke RA. Oncolytic virus therapy for cancer: the first wave of translational clinical trials. Translational Research. 2013;161(4):355-64.
Nabizadeh A, Bamdad T, Arefian E, Nikoo SHR. Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus. Iranian Journal of Cancer Prevention. 2016;9(1).
Chen N, Karantza-Wadsworth V. Role and regulation of autophagy in cancer. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 2009;1793(9):1516-23.
Takahashi Y, Coppola D, Matsushita N, Cualing HD, Sun M, Sato Y, et al. Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis. Nature cell biology. 2007;9(10):1142-51.
Itakura E, Kishi C, Inoue K, Mizushima N. Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Molecular biology of the cell. 2008;19(12):5360-72.
Liang C, Feng P, Ku B, Dotan I, Canaani D, Oh B-H, et al. Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG. Nature cell biology. 2006;8(7):688-98.
Hippert MM, O'Toole PS, Thorburn A. Autophagy in cancer: good, bad, or both? Cancer research. 2006;66(19):9349-51.
Meijer AJ, Codogno P. Regulation and role of autophagy in mammalian cells. The international journal of biochemistry & cell biology. 2004;36(12):2445-62.
Stanford MM, Barrett JW, Nazarian SH, Werden S, McFadden G. Oncolytic virotherapy synergism with signaling inhibitors: Rapamycin increases myxoma virus tropism for human tumor cells. Journal of virology. 2007;81(3):1251-60.
Sung CK, Choi B, Wanna G, Genden EM, Woo SL, Shin EJ. Combined VSV oncolytic virus and chemotherapy for squamous cell carcinoma. The Laryngoscope. 2008;118(2):237-42.
Schache P, Gürlevik E, Strüver N, Woller N, Malek N, Zender L, et al. VSV virotherapy improves chemotherapy by triggering apoptosis due to proteasomal degradation of Mcl-1. Gene therapy. 2009;16(7):849-61.
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