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Camelina Oil as a Promising Substrate for mcl-PHA Production in Pseudomonas sp. Cultures

Daniel Bustamante, Marta Tortajada, Daniel Ramon, Antonia Rojas
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Abstract

Background and objective: Polyhydroxyalkanoates are biodegradable polyesters synthesized by some prokaryotic organisms from renewable sources. Medium-chain-length Polyhydroxyalkanoates show interesting properties as elastic and adhesive specialty polymers. Medium-chain-length Polyhydroxyalkanoates producers such as Pseudomonas sp. have demonstrated high yields on fats and oils. Camelina sativa is non-food chain competing crop, whose seed contain about 43% (w w-1) oil in dry matter with about 90% (w w-1) of unsaturated fatty acids. Camelina oil was for the first time tested for the production of medium-chainlength Polyhydroxyalkanoates by different Pseudomonas strains.

Material and methods: The production of Polyhydroxyalkanoate was evaluated in a nitrogen-limited minimal medium supplemented with crude Camelina oil or saponified oil to compare the production capability of Pseudomonas sp. strains. A phosphates-limited medium was used to optimize polyhydroxyalkanoate production in fed-batch assays. Experiments were carried out by duplicates.

Results and conclusion: Pseudomonas resinovorans was used for direct fermentation of Camelina oil without prior hydrolysis. A first approach to process development in bioreactor has provided up to 40% (w w-1) polymer content, matching highest medium-chain-length polyhydroxyalkanoates titer reported from plant oils (13.2 g l-1). Camelina oil was shown to be a suitable substrate for production of medium-chain-length polyhydroxyalkanoates. This non-food vegetable oil gave good results for Pseudomonas resinovorans DSM 21078 without any pre-treatment.

Conflict of interest: The authors declare no conflict of interest.


Keywords

▪ Bioplastics ▪ Camelina oil ▪ Medium-chain-length polyhydroxyalkanoates ▪ PHA ▪ Pseudomonas sp.

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DOI: https://doi.org/10.22037/afb.v6i1.21635

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