Background and Objective: Recently, several researchers have shown the benefits deriving from probiotic products containing lactobacilli and bifidobacteria in their formulation. The purpose of this study was to develop a probiotic chocolate mousse using milk and soy milk in formulation with regard to survival of probiotic bacteria and sensorial acceptance during 21 days.
Material and Methods: Nine functional probiotic chocolate mousse formulations were produced by milk, milk/soy milk and soy milk and 3 probiotic strains (Lactobacillus acidophilus; Lactobacillus paracasei; Bifidobacteriumlactis). ThepH, acidity, survival of microbial strains, rheological and sensory properties of all treatments were monitored during 21 days refrigerated storage (4°C).
Results and Conclusion: The pH drop rate and acidity increase rate of all samples were significant during 21 days of storage (p≤0.05). There was a significant increase in the probiotic bacteria of all samples during 21 days of storage at 4°C (p≤0.05). However, the rate of probiotics growth was accelerated in formulation prepared with soy milk and milk/soy milk (1:1) in the chocolate mousse at day 7 and 14. Rheological experiment demonstrated that all samples known as viscoelastic solid dessert had shear-thinning behavior. In conclusion, chocolate dessert including soy milk as well as milk was shown to be more effective vehicle for delivery of probiotics, including Lactobacillus acidophilus, Lactobacillus paracasei, Bifidobacteriumlactis.
Conflict of interest: The authors declare no conflict of interest.
Khorshidian N, Yousefiasli M, Hosseini H, Shadnoush M, Mortazavian AM. Potential anticarcinogenic eﬀects of lactic acid bacteria and probiotics in detoxiﬁcation of process-induced food toxicants. Iran J Cancer Prev. 2016; 9(5), e7920. doi: 10.17795/ijcp-7920
Pandey KR, Naik SR, Vakil BV. Probiotics, prebiotics and synbiotics-Review. J Food Sci Technol. 2015; 52(12): 7577-7587. doi: 10.1007/s13197-015-1921-1.
Granato D, Branco GF, Cruz AG, Faria JAF, Shah NP. Probiotic dairy products as functional foods. CompR Rev Food Sci Food Saf. 2010; 9(5): 455-470. 10.1111/j.1541-4337.2010.00120.x.
Brouns F. Soya isoflavones: A new and promising ingredient for the health foods sector. Food Res Int. 2002; 187-193. doi:10.1016/S0963-9969(01)00182-X
Wang YC1, Yu RC, Chou CC. Antioxidative activities of soymilk fermented with lactic acid bacteria and bifidobacteria. Food Microbiol. 2006; 23(2): 128-35. doi: 10.1016/j.fm.2005.01.020.
Kazemi A, Mazloomi S, HassanzadehRostami Z, Akhlaghi M. Effect of adding soymilk on physicochemical, microbial and sensory characteristics of probiotic fermented milk containing Lactobacillus acidophilus. Iran J Vet Res. 2013; 48: 206-210.
Vinderola CG, Prosello W, Ghiberto D, Reinheimer JA. Viability of probiotic (Bifidobacterium, Lactobacillus acidophilus and Lactobacillus casei) and nonprobiotic microflora in Argentinian fresco cheese. J Dairy Sci. 2000; 83: 1905–1911.
Chen T, Wei Q, Chi Z. Effect of oligosaccharides and isoflavonesaglycones in defatted soy meal fermented by Lactobacillus paracasei and bifidobacterium longum. Afr J Microbiol. Res. 2011; 5(15): 1-9. doi: 10.5897/AJMR10.553
Tsangalis D, Shah NP. Metabolism of oligosaccharides and aldehydes and production of organic acids in soymilk by probiotic bifidobacteria. Int J Food Sci and Technol. 2004, 39, 541-554. doi: 10.1111/j.1365-2621.2004.00814.x.
Buriti FCA, Bedani R, Saad MIS. Probiotic and prebiotic dairy desserts. In: Watson., R.R., and Preedy, VR. editors. Probiotics, prebiotics and synbiotics, Elsevier Inc., 2016; 345-360. doi: 10.1016/B978-0-12-802189-7.00023-X
Casale Aragon-Alegroa L, Henrique Alarcon Alegrob J, Roberta-Cardarellib H, ChihChiub M, Marta IsaySaadb S. Potentially probiotic and symbiotic chocolate mousse. LWT-Food Sci Technol., 2006; 40: 669-675. doi: 10.1016/j.lwt.2006.02.020
Aboulfazli F, Salihin baba A, Misran M. Replacement of bovine milk with vegetable milk: Effects on the survival of probiotics and rheological and physicochemical properties of frozen fermented dessert. Int J Dairy Tech. 2014; 13: 1-27. doi: 10.1111/1471-0307.12219
Homayouni A, Azizi A, Javadi M, Mahdipour S, Ejtahed H. Factors influencing probiotic survival in ice cream. Int J Dairy Sci. 2012; 1-10. doi: 10.3923/ijds.2012.1.10
Lalicic-Petronijevic J, Popov-Raljic J, Obradovic D, Radulovic Z, Paunovic D, Petrrusic M, Pezo, L. Viability of probiotic strains Lactobacillus acidophilus NCFM and bifidobacteriumlactis HN019 and their impact on sensory and rheological properties of milk and dark chocolates during storage for 180 days. J Func Foods. 2015; 5: 541-550. doi: 10.1016/j.jff.2015.03.046
Richter Reis F, Bellarmino A, Lea J, Windson C. Bileski L. Apparent viscosity of a skim milk based dessert: optimization through respond surface methodology. Food Nutr Sci. 2011; 2: 90-95. doi:10.4236/fns.2011.22012
A.O.A.C. (2000). Official methods of analysis 17th. A.O.A.C. International, Gaitherburg, M.D.,
Sarvari F, Mortazavian A.M, Fazeli M.R. Biochemical Characteristics and Viability of Probiotic and Yogurt Bacteria in Yogurt during the Fermentation and Refrigerated Storage. Appl Food Biotechnol. 2014; 1(1): 55-61. doi: 10.22037/afb.v1i1.7125.
Pang Z, Deeth H, Sopade P, Sharma R. Bansal N. Rheology, texture and microstructure of gelatin gels with and without milk proteins. Food Hyrocoll. 2014; 35: 484-493. doi: 10.4236/fns.2011.22012.
Sarmento Valencia, M., Magalhaes Salgado, S., Alvachian Cardoso Andrade, S., Montarroyos Padilha V, Souza Livera AV, Montenegro Stamford TL. Development of creamy milk chocolate dessert added with fructo-oligosaccharide and Lactobacillus paracasei LBC 81, LWT-Food Sci Technol. 2016; 69, 104-109. doi: 10.1016/j.lwt.2016.01.039
Wang J, Guo Z, Zhang Q, Yan L, Chen W, Liu M, Zhang P. Fermentation characteristics and transit tolerance of Lactobacillus casei Zhang in soymilk and bovine milk during storage. J Dairy Sci. 2009; 92, 2468-2476. doi: 10.3168/jds.2008-1849
Oliveria D, Vidal L, Ares G, Walter EHM, Rosenthal A, Deliza R. Sensory, microbiological and physicochemical screening of probiotic cultures for the development of non-fermented probiotic milk. LWT-Food Sci Technol. 2017; 79: 234-241. doi: 10.1016/j.lwt.2017.01.020
Cruz AG, Antunes AEC, Sousa ALOP, Faria JAF, Saad SMI. Ice-cream as a probiotic food carrier. Food Res Int. 2009; 42: 1233-1239. doi: 10.1016/j.foodres.2009.03.020
Mahdian E, Karazhian R. Effects of fat replacers and stabilizers on rheological, physicochemical and sensory properties of reduced-fat ice cream. J Agric Sci Technol. 2013; 15: 1163-1174.
Adapa S, Dingeldein H, Schmidt KA. Rheological properties of ice cream mixes and frozen ice creams containing fat and fat replacers. J Dairy Sci. 2000; 83: 2224-2229. doi: 10.3168/jds.S0022-0302(00)75106-X.
Nishinari K, Fang Y, Guo S, Phillips G. Soy proteins: A review on composition, aggregation and emulsification. Food Hydrocoll. 2014; 39: 301-318. doi: 10.1016/j.foodhyd.2014.01.013.
Kaneko S, Kumazava K, Nishimura O. Studies on the key aroma compounds in soy milk made from three different soybean cultivars. J Agric Food Chem., 2011; 59(22): 12204-12209. doi.: 10.1021/jf202942h
Lozano PR, Dranke M, Benitez D, Cadwallader KR. Instrumental and sensory characterization of heat- induced odorants in aseptically packaged soy milk. J Agric Food Chem. 2007; 55(8): 3018–3026. doi: 10.1021/jf0631225