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Antibiofilm Effects of Lactobacilli against Ciprofloxacin-Resistant Uropathogenic Escherichia coli strains in Pasteurized Milk

Mahsa Yeganeh, Hedayat Hosseini, Sedigheh Mehrabian, Elham Siasi Torbati, Seyed Morteza Zamir
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Abstract

 

Background and Objective: Uropathogenic Escherichia coli-induced urinary tract infections are the most common uropathogenic Escherichia coli etiological agent. In addition, most of biofilms created by these bacteria can be regarded as a serious problem in the food industry. Foodborne diseases have always been considered an emerging public health concern throughout the world. Many outbreaks have been found to be associated with biofilms. Thus, the aim of the present study is to investigate the anti-adhesive effects of lactic acid bacteria against strains of Ciprofloxacin-Resistant Uropathogenic Escherichia coli using microbial techniques in pasteurized milk.

Material and Methods: In this study, strains of Lactobacillus plantarum, Lactobacillus casei and Lactobacillus acidophilus were provided from Pasteur Institute of Iran. Twenty strains of Uropathogenic Escherichia coli-Induced Urinary Tract Infections were isolated from patients with urinary tract infection in Shahid Labbafinejad hospital of Iran. Eight strains with ability of biofilm formation were selected for microbial tests. All of these eight strains were resistant to ciprofloxacin. Disk diffusion method was used to assess the susceptibility of all isolates to the ten common antibiotics. Eight samples of Uropathogenic Escherichia coli were inoculated in pasteurized milk. The microtitre plate 100 method was used to detect anti-adhesive activity of lactobacilli supernatant.

Results and Conclusion: Results showed that the eight human isolates were resistant to antibiotics. Isolate of number 4 was the most susceptible strains to antibiofilm effects of lactobacilli in the pasteurized milk. The anti-adhesive effects of lactobacilli on Uropathogenic were confirmed in all microbial tests. In this study, Lactobacillus plantarum revealed the highest inhibitory activity against Uropathogenic Escherichia coli 4 strain with inhibition zones of 42 mm. This strain was reported as a proper probiotic bacterium. According to the results, these lactobacilli have had spectacular effects on biofilm formation and pathogenicity of Uropathogenic strains to prevent the adhesion.

Conflict of interest: The authors declare no conflict of interest.


Keywords

▪ Antibiofilm effects  ▪ Ciprofloxacin-resistant ▪ Lactobacillus acidophilus ▪ Lactobacillus casei ▪ Lactobacillus plantarum ▪Uropathogenic Escherichia coli

References

Paton JC, Paton AW. Pathogenesis and diagnosis of Shiga toxin producing Escherichia coli infections. Clin Microbiol Rev. 1998; 11:450-479.

McClure P. Escherichia coli: Virulence, stress response and resistance.Pages in Understanding Pathogen Behavior. M.Griffiths, ed. CRC Press LLC, Boca Raton, FL, 2005: 240-278

Price SB, Wright JC, DeGraves FJ, Castanie-Cornet MP, Foster JW. Acid resistance systems required for survival of Escherichia coli H7:O157 in the bovine gastrointestinal tract and in apple cider are different. Appl Environ Microbiol. 2004; 70:4792–4799.

Wiles TJ, Kulesus RR, Mulvey MA. Origins and Virulence Mechanisms of Uropathogenic Escherichia coli. Exp Mol Pathol. 2008; 85(1):11–19. doi: 10.1016/j.yexmp.2008.03.007

Kanjee U, Houry WA. Mechanisms of acid resistance in Escherichia coli. Annu Rev Microbiol. 2013; 67: 65– 81. doi: 10.1146/annurev-micro-092412-155708.

Oh DH, Pan Y, Berry E, Cooley M, Mandrell R, Breidt FJR. Escherichia coli H7:O157 strains isolated from environmental sources differ significantly in acetic acid resistance compared with human outbreak strains. J Food Prot. 2009;72(3):503–509.

Kim G-H, Breidt F, Fratamico P, Oh D-H. Acid resistance and molecular characterization of Escherichia coli H7:O157 and different non-O157 Shiga toxin producing E. coli serogroups. J Food Sci . 2015; 80(10): M2257–M2264. doi: 10.1111/1750-3841.12996.

Jensen PQ, Givskov M, Bjarnsholt T, Moser C. The immune system vs Pseudomonas aeruginosa biofilms. FEMS Immunol Med Microbiol. 2010; 59(3):292–305. doi: 10.1111/j.1574-695X.2010.00706.x.

Sawhney R, Berry V. Bacterial biofilm formation, pathogenicity, diagnostics and control: an overview. Indian J Med Sci. 2009;63(7):313–321. doi: 10.4103/0019-5359.55113.

O’Toole G, Kaplan HB, Kolter R. Biofilm formation as microbial development. Annu Rev Microbiol. 2000; 54: 49–79.

Drenkard E, Ausubel FM. Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation. Nature. 2002;416:740–743.

Gupta V, Garg R. Probiotics. Indian J Med Microbiol. 2009; 27:202–209

Rodrigues L, Banat IM, Teixeira J, Oliveira R. Biosurfactants: potential applications in medicine. J Antimicrob Chemother. 2006;57:609–618.

Kenreigh CA, Wagner LT. Probiotic supplements ease diarrhea and other gastrointestinal disorders. Medscape Pharm. 2006; 12:14–20.

Saira B, Yasra S, Aamir A, Mashkoor M, Muhammad Azeem S, Ayesha T, and Abdul H. multiple drug resistance patterns in various phylogenetic groups of Uropathogenic E. coli isolated from faisalabad region of Pakistan. Braz J Microbiol. 2011 Oct; 42(4):1278-83. doi: 10.1590/S1517-83822011000400005

Bukh AS, Schonheyder HC, Emmersen JM, Sogaard M,Bastholm S, Roslev P. Escherichia coli phylogenetic groups are associated with site of infection and level of antibiotic resistance in community-acquired bacteraemia: a 10 year population-based study in Denmark. J Antimicrob Chemother. 2009 Jul;64(1):163-8. doi: 10.1093/jac/dkp156.

Bonkat G, Müller G, Braissant O, Frei R, Tschudin-Suter S, Rieken M, Wyler S, Gasser TC, Bachmann A, Widmer AF. Increasing prevalence of ciprofloxacin resistance in extended-spectrum-beta-lactamase-producing Escherichia coli urinary isolates. World J Urol. 2013;31:1427-32. doi: 10.1007/s00345-013-1031-5.

- Corkill JE, Anson JJ, Hart CA. High prevalence of the plasmid-mediated quinolone resistance determinant qnrA in multidrug- resistant Enterobacteriaceae from blood cultures in Liverpool, UK. J Antimicrob Chemotherap. 2005;56:1115-7

- Putnam SD, Sanders JW, Tribble DR, Rockabrand DR, Riddle MS, Rozmajzl PJ, Frenck RW. Posttreatment changes in Escherichia coli antimicrobial susceptibility rates among diarrheic patients treated with ciprofloxacin. Antimicrob Agent Chemotherap. 2005; 49:2571-2. doi:10.1128/AAC.49.6.2571–2572.2005

Soleimani NA, Kermanshahi RK, Yakhchali B, Sattari TN. Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis. African J Microbiol Res. 2010; 4 (20): 2169- 2173.

Salleh F, Lani M.N, Ismail N, Aween M.M, Alias R. Identification of Lactic Acid Bacteria Species Isolated from Fermented Durian Flesh (Tempoyak) in Kuala Terengganu and Marang Markets and Their Antimicrobial Activities Against Selected Pathogenic Bacteria. Proceedings of the International Conference on Beneficial Microbes ICOBM . 2014; 214-218

Shah NP, Dave R. Antimicrobial substances including bacteriocins produced by lactic acid bacteria. Biosci Microflora. 2002; 21 (4):217-223.

Stepanović S, Vuković D, Hola V, Di Bonaventura G, Djukić S, Cirković I, Ruzicka F.Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. Acta Pathol Microbiol Immunol Scand. 2007; 115 (8): 891- 9.

Spinler JK, Taweechotipatr M, Rognerud CL, Ou CN, Tumwasorn S, Versalovic J. Human- derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe. 2008; 14 (3): 166- 71. doi: 10.1016/j.anaerobe.2008.02.001.

Flint S, Palmer J, Bloemen K, Brooks J, Crawford R . The growth of Bacillus stearothermophilus on stainless steel. J Appl Microbiol. 2001; 90(2):151–7.

Abedi D, Feizizadeh S, Akbari V, Jafarian-Dehkordi A. In vitro anti bacterial and anti- adherence effects of Lactobacillus delbrueckii subsp bulgaricus on Escherichia coli. Res Pharm Sci. 2013; 8(4):260-8.




DOI: https://doi.org/10.22037/afb.v4i4.17783

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