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Microbial Protein Production from Candida tropicalis ATCC13803 in a Submerged Batch Fermentation Process

Sahar Golaghaiee, Fatemeh Ardestani, Hamid Reza Ghorbani




Background and Objective: Microbial protein production can resolve one of the major world challenges, i.e. lack of protein sources. Candida tropicalis growth was investigated to specify a medium to reach the highest cell proliferation and protein production.
Material and Methods: Fractional factorial design and the index of signal to noise ratio were applied for optimization of microbial protein production. Optimization process was conducted based on the experimental results of Taguchi approach designs. Fermentation
was performed at 25oC and the agitation speed of 300 rpm for 70 h. Ammonium sulfate, iron sulfate, glycine and glucose concentrations were considered as process variables. Optimization of the culture medium composition was conducted in order to obtain the highest cell biomass concentration and protein content. Experiment design was performed based on the Taguchi approach and L-16 orthogonal arrays using Qualitek-4 software.
Results and Conclusion: Maximum biomass of 8.72 log (CFU ml-1) was obtained using the optimized medium with 0.3, 0.15, 2 and 80 g l-1 of ammonium sulfate, iron sulfate, glycine and glucose, respectively. Iron sulfate and ammonium sulfate with 41.76% (w w-1) and 35.27% (w w-1) contributions, respectively, were recognized as the main components for cell growth. Glucose and glycine with 17.12% and 5.86% (w w-1) contributions,
respectively, also affected cell production. The highest interaction severity index of +54.16% was observed between glycine and glucose while the least one of +0.43% was recorded for ammonium sulfate and glycine. A deviation of 7% between the highest
predicted cell numbers and the experimented count confirms the suitability of the applied statistical method. High protein content of 52.16% (w w-1) as well as low fat and nucleic acids content suggest that Candida tropicalis is a suitable case for commercial processes.
Conflict of interest: The authors declare that there is no conflict of interest.


▪ Candida tropicalis ▪ Cell growth ▪ Medium composition ▪ Protein content ▪ Taguchi approach


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DOI: https://doi.org/10.22037/afb.v4i1.13698


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