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Microbial Protein Production from Candida tropicalis ATCC13803 in a Submerged Batch Fermentation Process

Sahar Golaghaiee, Fatemeh Ardestani, Hamid Reza Ghorbani
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Abstract

Background and Objective: Microbial protein production can resolve one of the major world challenges, i.e. lack of protein sources. Candida tropicalis growth was investigated to specify a medium to reach the highest cell proliferation and protein production.
Material and Methods: Fractional factorial design and the index of signal to noise ratio were applied for optimization of microbial protein production. Optimization process was conducted based on the experimental results of Taguchi approach designs. Fermentation
was performed at 25oC and the agitation speed of 300 rpm for 70 h. Ammonium sulfate, iron sulfate, glycine and glucose concentrations were considered as process variables. Optimization of the culture medium composition was conducted in order to obtain the highest cell biomass concentration and protein content. Experiment design was performed based on the Taguchi approach and L-16 orthogonal arrays using Qualitek-4 software.
Results and Conclusion: Maximum biomass of 8.72 log (CFU ml-1) was obtained using the optimized medium with 0.3, 0.15, 2 and 80 g l-1 of ammonium sulfate, iron sulfate, glycine and glucose, respectively. Iron sulfate and ammonium sulfate with 41.76% (w w-1) and 35.27% (w w-1) contributions, respectively, were recognized as the main components for cell growth. Glucose and glycine with 17.12% and 5.86% (w w-1) contributions,
respectively, also affected cell production. The highest interaction severity index of +54.16% was observed between glycine and glucose while the least one of +0.43% was recorded for ammonium sulfate and glycine. A deviation of 7% between the highest
predicted cell numbers and the experimented count confirms the suitability of the applied statistical method. High protein content of 52.16% (w w-1) as well as low fat and nucleic acids content suggest that Candida tropicalis is a suitable case for commercial processes.
Conflict of interest: The authors declare that there is no conflict of interest.


Keywords

▪ Candida tropicalis ▪ Cell growth ▪ Medium composition ▪ Protein content ▪ Taguchi approach

References

Anupama, Ravindra P. Value-added food: single cell protein. Biotechnol Adv. 2000; 18(6): 459-479.

Adedayo MR, Ajiboye EA, Akintunde JK, Odaibo A. Single

cell proteins: As nutritional enhancer. Adv Appl Sci Res.

; 2(5): 396-409.

Nasseri AT, Rasoul-Amini S, Morowvat MH, Ghasemi Y.

Single cell protein: production and process. Am J Food

Technol. 2011; 6: 103-116. doi: 10.3923/ajft.2011.103.116

Gomashe AV, Pounikar MA, Gulhane PA. Liquid whey: a

potential substrate for single cell protein production from

Bacillus subtilis NCIM. Int J Life Sci. 2014; 2(2): 119-123.

Santos EO, Michelon M, Furlong EB, Burkert JFM, Kalil SJ,

Burkert CAV. Evaluation of the composition of culture

medium for yeast biomass production using glycerol from

biodiesel synthesis. Braz J Microbiol. 2012; 43(2): 432-440.

Nigam NM. Cultivation of Candida langeronii in sugar cane bagasse hemi cellulose hydrolysate for the production of single cell protein. World J Microbiol Biotechnol. 2000; 16(4): 367-372. doi: 10.1023/A:1008922806215

Rudravaram R, Rao LV, Pogaku R. Studies on Aspergillus

oryzae mutants for the production of single cell proteins from deoiled rice bran. Food Technol Biotechnol. 2003; 41 (3): 243-246.

Gabriel A, Ntuli V, James D. C1Cactus pear biomass, a

potential lignocellulose raw material for single cell protein

production (SCP). Int J Curr Microbiol Appl Sci. 2014; 3(7):

-197.

Zheng S, Yang M, Yang Z. Biomass production of yeast

isolate from salad oil manufacturing wastewater. Bioresour

Technol. 2005; 96 (10): 1183–1187. doi: 10.1016/j.biortech. 2004.09.022

Bankra AV, Kumar AR, Zinjarde SS. Environmental and

industrial applications of Yarrowia lipolytica. J Appl

Microbiol Biotechnol. 2009; 84(5): 847-865. doi:10.1007-

s00253-009-2156-8

Garcia-Garibay M, Gomez-Ruiz L, Cruz-Guerrero AE,

Barzana E. Single cell protein: The algae. Encyc Food

Microbiol. 2014: 425-430.

Nalage DN, Khedkar GD, Kalyankar AD, Sarkate AP,

Ghodke SR, Bedre VB, Khedkar CD. Single cell proteins.

Encyc Food Health. 2016: 790-794.

Raja R, Hemaiswarya S, Ashok Kumar N, Sridhar S,

Rengasamy R. A perspective on the biotechnological potential of microalgae. Crit Rev Microbiol. 2008; 34(2): 77-88.

Ardestani F, Alishahi F. Optimization of single cell protein production by Aspergillus niger using Taguchi approach. J Food Biosci Technol. 2015; 5(2): 73-79.

Gour S, Mathur N, Singh A, Bhatnagar P. Single cell protein production: A review. Int J Curr Microbiol Appl Sci. 2015; 4(9): 251-262.

Liu Z, Weis R, Glieder A. Enzymes from higher eukaryotes for industrial biocatalysis. Food Technol Biotechnol. 2004; 42(4): 237-249.

Bureik M, Schiffler B, Hiraoka Y, Vogel F, Bernhardt R.

Functional expression of human mitochondrial CYP11B2 in

fission yeast and identification of a new internal electron

transfer protein etp1. Biochem. 2002; 41(7): 2311-2321.

V. Kamzolova S, Morgunov IG, Aurich A, Perevoznikova

OA, Shishkanova NV, Stottmeister U, Finogenova TV.

Lipase secretion and citric acid production in Yarrowia

lipolytica yeast grown on animal and vegetable fat. Food

Technol Biotechnol. 2005; 43: 113-122.

Argyro B, Costas P, Athanasios AK. Production of food grade yeasts. Food Technol Biotechnol. 2006; 44(3): 407-415.

Kasprowicz-Potocka M, Zaworska A, Frankiewicz A, Nowak W, Gulewicz P, Zdunczyk Z, Juskiewicz J. The nutritional value and physiological properties of diets with raw and Candida utilis-fermented lupin seeds in rats. Food Technol Biotechnol. 2015; 53(3): 286-297.

Kargi F, Shuler ML, Vashon R, Seeley HW, Henry A, Austic RE. Continuous aerobic conversion of poultry waste into single-cell protein using a single reactor: Kinetic analysis and determination of optimal conditions. Biotechnol Bioeng J. 2004; 22(8): 1567-1600. doi: 10.1002/bit.260220805

Prado-Rubio OA, Jorgensen JB, Jorgensen SB. Systematic model analysis for single cell protein (SCP), production in a U-loop reactor. Comput Aided Chem Eng. 2010; 28: 319-324. doi: 10.1016/S1570-7946(10)28054-9.

Cayabyab VA, Uyenco FR, Rosario EJD. Batch culture

production of Candida tropicalis in acid hydrolyzate of rice

straw. Philippine J Crop Sci. 1976; 2(4): 238-244.

Miller GL. Use of dinitrosalicylic acid regent for determination of reducing sugar. Anal Chem. 1959; 31(3): 426-428.

Rahnamaei M. Cereals and pulses- Determination of the nitrogen content and calculation of the crude protein content-Kjeldahl method. Iran Nat Standard Org. 2015; INSO 19052:2-9.

Chomczynski P, Sacchi N. Single-step method of RNA

isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal Chem. 1987; 162(1): 156-159.

Barnes H, Balckstock J. Estimation of lipids in marine

animals and tissue: detailed investigation of the sulphophosphovanilun method for total lipids. J Exp Mar Biol Ecol.1973; 12(1): 103-118.

Dhanasekaran D, Lawanya S, Saha S, Thajuddin N,

Panneerselvam A. Production of single cell protein from pineapple waste using yeast. Innov Rom Food Biotechnol.

; 8: 26-32.

Jaganmohan P, Purushottam B, Prasad SV. Production of single cell protein (SCP) with Aspergillus terreus using solid state fermentation. Eur J Biol Sci. 2013; 5(2): 38-43.

Singh A, Abidi AB, Aqrawal AK, Darmwal NS. Single cell

protein production by Aspergillus niger and its evaluation.

Zbl Mikrobiol. 1991; 146(3): 181-184.

Bacha U, Nasir M, Khalique A, Anjum AA, Jabbar MA.

Comparative assessment of various agro-industrial wastes for Saccharomyces cerevisiae biomass production and its quality production: as a single cell protein. J Anim Plant Sci. 2011; 21(4): 844-849.

Khan M, Saeed Khan S, Ahmed Z, Tanveer A. Production of single cell protein from Saccharomyces cerevisiae by utilizing fruit wastes. Nanobiotech Uni. 2010; 1(2): 127-132.

Yousufi MK. To determine protein content of single cell

protein produced by using various combinations of fruit

wastes in the production of SCP by using two standard food fungi Aspergillus oryzae and Rhizopus oligospora. Int J Adv Biotechnol Res. 2012; 3(1): 533-536.

Schultz N, Chang L, Hauck A, Reuss M, Syldatk C.

Microbial production of single-cell protein from deproteinized whey concentrates. Appl Microbiol Biotechnol. 2006; 69(5): 515-520. doi: 10.1007/s00253-005-0012-z




DOI: https://doi.org/10.22037/afb.v4i1.13698

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