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Selective Toxicity of Standardized Extracts of Persian Gulf Sponge ( Irciniamutans) on Skin Cells and Mitochondria isolated from Melanoma induced mouse

Yalda Arast, Jalal Pourahmad
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Abstract

Melanoma is an aggressive and highly lethal cancer with poor prognosis and resistance to current treatments. Apoptosis signaling is believed to be suppressed in melanoma. Evidence suggests that compounds isolated from marine sponges have anti-cancer properties. This study was designed to evaluate the apoptotic effect of methanolic, diethyl ether, and n-hexane extracts of Irciniamutans (I.mutans) on skin mitochondria isolated from mice animal models of melanoma. Mitochondria were isolated by differential centrifugation. According to our results, methanolic, diethyl ether, and n-hexane extracts of I.mutans raised the reactive oxygen species (ROS) level only in the cancerous skin mitochondria group. Furthermore, methanolic, diethyl ether, and n-hexane extracts induced swelling in the mitochondria, decreased the mitochondrial membrane potential (MMP) and the release of cytochrome c from the mitochondria. Based on these results, the potentially bioactive compounds found in I.mutans render the sea sponge as a strong candidate for further researches in molecular identification and confirmatory in-vivo researches.

Key Words:Irciniamutans; Melanoma; Apoptosis; Mitochondria; Reactive Oxygen Species.


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DOI: https://doi.org/10.22037/ipa.v1i3.25103

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