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Mice fetus liver tissue spectroscopic alterations following concomitant administration of Metronidazole & Miconazole

Azadeh Ashtarinezhad, Ataollah Panahyab, Farshad H. Shirazi
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Abstract

Introduction: Metronidazole (MET) and Miconazole (MIC) are antiprotozoal and imidazole antifungal agents, respectively. The aim of this study was to assess the usefulness of Fourier Transform Infrared micro-spectroscopy (FTIR-MSP) for discriminating and detecting spectral changes of mouse fetus liver tissue after mother’s exposure to concomitant use of MET and MIC.

Methods and Results: The control group received only normal diet and the exposure groups received MET, MIC, and concomitant MET and MIC. The pregnant mice were anesthetized and their fetuses were surgically removed on gestation day 15. Fixative slides without any staining were put on KBr disc with a 1mm thick for IR micro spectroscopy. All information obtained from the spectra were analyzed using routine FTIR software. Based on our results, MET and MIC, alone and in combination together, have created some changes in the mice fetus liver biomolecules.

Conclusions: FTIR biospectroscopy application in teratology is very challenging, but it has been accepted as a reliable technique; however, it is necessary to do more investigations.


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DOI: https://doi.org/10.22037/ipa.v1i3.22244

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