• Logo
  • SBMUJournals

The Effect of Short-Term Treadmill Exercise on the Expression Level of TFAM in the Heart of Nicotine-Sensitized Rats

Amir Abbas Lashgari, Mohammad-Ali Azarbayjani, Maghsoud Peeri, Mohammad Nasehi
0

Views

PDF

Abstract

Introduction: TFAM (mitochondrial transcription factor A) is involved in mitochondrial biogenesis and induces anti-oxidant and anti-apoptotic effects. Nicotine can also alter the function of cardiovascular system and induce heart failure and other heart diseases. Interestingly, it has been reported that exercise can interfere with the effects of nicotine, and change the expression pattern of different genes. The goal of the present study was to investigate the effect of short-term treadmill exercise on the expression level of TFAM in the heart of nicotine-sensitized rats.

Materials and Methods: Nicotine was administered intraperitoneally at the dose of 0.21 mg/kg. Treadmill exercise was performed during 14 days, according to the study’s protocol.

Results: The results revealed that nicotine reduced the expression of TFAM. The treadmill (Fourteen-day training) increased the expression of TFAM in the heart of the control rats. Furthermore, 14-day training with treadmill restored the effect of nicotine on the expression of TFAM in nicotine-sensitized rats.

Conclusion: Nicotine induced pro-apoptotic and anti-oxidative stress effects via down-regulating the expression of TFAM. Fourteen -day training with treadmill induced a protective effect against nicotine-induced cardiac apoptosis and oxidative stress, via restoring the effect of nicotine on TFAM. The results are indicative of the fact that short-term treadmill exercise may decrease the risk of heart failure and other cardiac diseases. 

 


Keywords

TFAM (mitochondrial transcription factor A), Nicotine, Treadmill, Heart, Rats

References

Theilen NT, Kunkel GH, Tyagi SC, The Role of Exercise and TFAM in Preventing Skeletal Muscle Atrophy. J Cell Physiol. 2017; 232(9): 2348-58.

Kukat C, Larsson NG, mtDNA makes a U-turn for the mitochondrial nucleoid. Trends Cell Biol. 2013; 23(9): 457-63.

Canugovi C, Maynard S, Bayne AC, Sykora P, Tian J, de Souza-Pinto NC, et al., The mitochondrial transcription factor A functions in mitochondrial base excision repair. DNA Repair (Amst). 2010; 9(10): 1080-9.

Nasehi M, Torabinejad S, Hashemi M, Vaseghi S, Zarrindast MR, Effect of cholestasis and NeuroAid treatment on the expression of Bax, Bcl-2, Pgc-1alpha and Tfam genes involved in apoptosis and mitochondrial biogenesis in the striatum of male rats. Metab Brain Dis. 2019.

Yokoyama M, Okada S, Nakagomi A, Moriya J, Shimizu I, Nojima A, et al., Inhibition of endothelial p53 improves metabolic abnormalities related to dietary obesity. Cell Rep. 2014; 7(5): 1691-703.

Kunkel GH, Chaturvedi P, Thelian N, Nair R, Tyagi SC, Mechanisms of TFAM-mediated cardiomyocyte protection. Can J Physiol Pharmacol. 2018; 96(2): 173-81.

Xiao D, Wang L, Huang X, Li Y, Dasgupta C, Zhang L, Protective Effect of Antenatal Antioxidant on Nicotine-Induced Heart Ischemia-Sensitive Phenotype in Rat Offspring. PLoS One. 2016; 11(2): e0150557.

Lawrence J, Chen M, Xiong F, Xiao D, Zhang H, Buchholz JN, et al., Foetal nicotine exposure causes PKCepsilon gene repression by promoter methylation in rat hearts. Cardiovasc Res. 2011; 89(1): 89-97.

Coelho FO, Jorge LB, de Braganca Viciana AC, Sanches TR, Dos Santos F, Helou CMB, et al., Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in klotho-haploinsufficient mice. Am J Physiol Renal Physiol. 2018; 314(5): F992-F98.

Liang D, Wang KJ, Tang ZQ, Liu RH, Zeng F, Cheng MY, et al., Effects of nicotine on the metabolism and gene expression profile of SpragueDawley rat primary osteoblasts. Mol Med Rep. 2018; 17(6): 8269-81.

Godoy JA, Valdivieso AG, Inestrosa NC, Nicotine Modulates Mitochondrial Dynamics in Hippocampal Neurons. Mol Neurobiol. 2018; 55(12): 8965-77.

Park SS, Shin MS, Park HS, Kim TW, Kim CJ, Lim BV, Treadmill exercise ameliorates nicotine withdrawal-induced symptoms. J Exerc Rehabil. 2019; 15(3): 383-91.

Zhou Y, Li C, Li R, Zhou C, Exercise improves nicotine reward-associated cognitive behaviors and related alpha7 nAChR-mediated signal transduction in adolescent rats. J Cell Physiol. 2018; 233(8): 5756-67.

Li FH, Yu HT, Xiao L, Liu YY, Response of BAX, Bcl-2 Proteins, and SIRT1/PGC-1alpha mRNA Expression to 8-Week Treadmill Running in the Aging Rat Skeletal Muscle. Adv Exp Med Biol. 2016; 923: 283-89.

Alipour V, Shabani R, Zarrindast MR, Rahmani-Nia F, Nasehi M, Influence of Moderate and Severe Exercise on Memory Formation and Anxiety-like Behaviors in Male Wistar Rat. Journal of Paramedical Sciences. 2019; 10(1): 27-33.

Karamanlidis G, Nascimben L, Couper GS, Shekar PS, del Monte F, Tian R, Defective DNA replication impairs mitochondrial biogenesis in human failing hearts. Circ Res. 2010; 106(9): 1541-8.

Marin-Garcia J, Goldenthal MJ, Mitochondrial centrality in heart failure. Heart Fail Rev. 2008; 13(2): 137-50.

Naka KK, Vezyraki P, Kalaitzakis A, Zerikiotis S, Michalis L, Angelidis C, Hsp70 regulates the doxorubicin-mediated heart failure in Hsp70-transgenic mice. Cell Stress Chaperones. 2014; 19(6): 853-64.

Kunkel GH, Chaturvedi P, Tyagi SC, Mitochondrial pathways to cardiac recovery: TFAM. Heart Fail Rev. 2016; 21(5): 499-517.

Ikeda M, Ide T, Fujino T, Arai S, Saku K, Kakino T, et al., Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress. PLoS One. 2015; 10(3): e0119687.

Russell LK, Mansfield CM, Lehman JJ, Kovacs A, Courtois M, Saffitz JE, et al., Cardiac-specific induction of the transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator-1alpha promotes mitochondrial biogenesis and reversible cardiomyopathy in a developmental stage-dependent manner. Circ Res. 2004; 94(4): 525-33.

Ikeuchi M, Matsusaka H, Kang D, Matsushima S, Ide T, Kubota T, et al., Overexpression of mitochondrial transcription factor a ameliorates mitochondrial deficiencies and cardiac failure after myocardial infarction. Circulation. 2005; 112(5): 683-90.

Slotkin TA, Skavicus S, Card J, Stadler A, Levin ED, Seidler FJ, Developmental Neurotoxicity of Tobacco Smoke Directed Toward Cholinergic and Serotonergic Systems: More Than Just Nicotine. Toxicol Sci. 2015; 147(1): 178-89.

Duncan JR, Randall LL, Belliveau RA, Trachtenberg FL, Randall B, Habbe D, et al., The effect of maternal smoking and drinking during pregnancy upon (3)H-nicotine receptor brainstem binding in infants dying of the sudden infant death syndrome: initial observations in a high risk population. Brain Pathol. 2008; 18(1): 21-31.

Feng Y, Caiping M, Li C, Can R, Feichao X, Li Z, et al., Fetal and offspring arrhythmia following exposure to nicotine during pregnancy. J Appl Toxicol. 2010; 30(1): 53-8.

Mandl A, Huong Pham L, Toth K, Zambetti G, Erhardt P, Puma deletion delays cardiac dysfunction in murine heart failure models through attenuation of apoptosis. Circulation. 2011; 124(1): 31-9.

Lee Y, Gustafsson AB, Role of apoptosis in cardiovascular disease. Apoptosis. 2009; 14(4): 536-48.

Wang L, Li X, Zhou Y, Shi H, Xu C, He H, et al., Downregulation of miR-133 via MAPK/ERK signaling pathway involved in nicotine-induced cardiomyocyte apoptosis. Naunyn Schmiedebergs Arch Pharmacol. 2014; 387(2): 197-206.

Zhou X, Sheng Y, Yang R, Kong X, Nicotine promotes cardiomyocyte apoptosis via oxidative stress and altered apoptosis-related gene expression. Cardiology. 2010; 115(4): 243-50.

Sinha-Hikim I, Friedman TC, Falz M, Chalfant V, Hasan MK, Espinoza-Derout J, et al., Nicotine plus a high-fat diet triggers cardiomyocyte apoptosis. Cell Tissue Res. 2017; 368(1): 159-70.

Lagouge M, Argmann C, Gerhart-Hines Z, Meziane H, Lerin C, Daussin F, et al., Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha. Cell. 2006; 127(6): 1109-22.

Kanter M, Aksu F, Takir M, Kostek O, Kanter B, Oymagil A, Effects of Low Intensity Exercise Against Apoptosis and Oxidative Stress in Streptozotocin-induced Diabetic Rat Heart. Exp Clin Endocrinol Diabetes. 2017; 125(9): 583-91.

Darband SG, Sadighparvar S, Yousefi B, Kaviani M, Mobaraki K, Majidinia M, Combination of exercise training and L-arginine reverses aging process through suppression of oxidative stress, inflammation, and apoptosis in the rat heart. Pflugers Arch. 2019.

Lee I, Huttemann M, Kruger A, Bollig-Fischer A, Malek MH, (-)-Epicatechin combined with 8 weeks of treadmill exercise is associated with increased angiogenic and mitochondrial signaling in mice. Front Pharmacol. 2015; 6: 43.




DOI: https://doi.org/10.22037/aab.v11i1.28573

Refbacks

  • There are currently no refbacks.