Assessment of the safety of chicken egg yolk antibody (IgY) consumption by lipid peroxidation marker in mice
Archives of Advances in Biosciences,
Vol. 8 No. 3 (2017),
13 June 2017
,
Page 29-35
https://doi.org/10.22037/jps.v8i3.17032
Abstract
Production of antibodies in chickens (IgY) has significantly attracted attention of scientists. Numerous publications have reported use of IgY in diagnosis, therapy and prophylaxis. Production of antigen-specific antibodies in chicken can help treat and prevent infectious diseases. The aim of this study was to assess the safety of IgY( anti E. coli O157:H7) on the antioxidant system in mice .Therefore in this study, three different doses of IgY against E. coli O157:H7 (0.9375, 1.875 and 3.75 g / kg) were administrated through oral route to 18 mice (treated groups) and PBS to the control group and 14 days after administration, blood samples were collected from the mice. Serum malondialdehyde (MDA) level and catalase, glutathione peroxidase and superoxide dismutase activity were measured using commercial kits. Oral administration of IgY against E. coli O157:H7 in doses of 0.9375, 1.875 and 3.75 g / kg caused no deaths and showed no toxic effects on mice. In this study, after 14 days of IgY administration there were no significant changes in the activity of antioxidant enzymes (catalase, glutathione peroxidase and superoxide dismutase) and MDA serum level compared to the control group. Our findings revealed that oral administration of IgY against E. coli O157:H7 does not show any toxic effects and does not disturb the antioxidant system in mice . These findings could be indicative of safety of oral administration of IgY in mice.
How to Cite
References
Jones KE, Patel NG, Levy MA, Storeygard A, Balk D, Gittleman JL, et al. Global trends in emerging infectious diseases. Nature. 2008;451(7181):990-3.
Couig M. Overview and summary: Infectious diseases: Challenges and solutions. Online Journal of Issues in Nursing. 2006;11(1).
Kovacs-Nolan J, Mine Y. Egg yolk antibodies for passive immunity. Annual review of food science and technology. 2012;3:163-82.
Guimarães MCC, Amaral LG, Rangel LBA, Silva IV, Matta CGF, de Matta MFR. Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies. Archivum immunologiae et therapiae experimentalis. 2009;57(5):377-82.
-Sampaio LCL, Baldissera MD, Sagrillo MR, Heres TDS, Oliveira CB, Stainki DR, et al. In vitro cytotoxicity and genotoxicity of chicken egg yolk antibody(IgY) against trypanosoma evansi in human lymphocytes . Pharmacy and Pharmaceutical Sciences 2014; 6(3).
Carlander D, Kollberg H, Wejåker P-E, Larsson A. Peroral immunotheraphy with yolk antibodies for the prevention and treatment of enteric infections. Immunologic research. 2000;21(1):1-6.
Carlander D. Avian IgY Antibody: in vitro and in vivo: Acta Universitatis Upsaliensis; 2002;53
Larsson A, Karlsson-Parra A, Sjöquist J. Use of chicken antibodies in enzyme immunoassays to avoid interference by rheumatoid factors. Clinical chemistry. 1991;37(3):411-4.
Akita E, Nakai S. Immunoglobulins from egg yolk: isolation and purification. Journal of food science. 1992;57(3):629-34.
Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology. 2003;189(1):41-54.
Radak Z, Chung HY, Goto S. Systemic adaptation to oxidative challenge induced by regular exercise. Free Radical Biology and Medicine. 2008;44(2):153-9.
Michiels C, Raes M, Toussaint O, Remacle J. Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free Radical Biology and Medicine. 1994;17(3):235-48.
MatÉs JM, Pérez-Gómez C, De Castro IN. Antioxidant enzymes and human diseases. Clinical biochemistry. 1999;32(8):595-603.
Dringen R. Metabolism and functions of glutathione in brain. Progress in neurobiology. 2000;62(6):649-71.
Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, metabolism and cardiovascular diseases. 2005;15(4):316-28.
Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radical Biology and Medicine. 1991;11(1):81-128.
Ikemori Y, Ohta M, Umeda K, Icatlo FC, Kuroki M, Yokoyama H, et al. Passive protection of neonatal calves against bovine coronavirus-induced diarrhea by administration of egg yolk or colostrum antibody powder. Veterinary microbiology. 1997;58(2-4):105-11.
Sudjarwo SA, Indriyani W, Nasronudin N, Sudjarwo GW. Acute Oral Toxicity of Immunoglobulin Y (IgY) anti HIV in Mice. 2015 ; 5 (02): 123-126.
- Abstract Viewed: 683 times
- PDF Downloaded: 296 times