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A method of GC-MS analysis of serum metabolites

Masoud Soheili, Afsaneh Arefi Oskuei, Mostafa Rezaei Tavirani, Seyyed Mojtaba Banitaba, Ghazaleh Aliakbarzadeh, Mahmoud Salami




Metabolites are the intermediate and natural low molecular weight products of metabolic reactions that naturally occur within cells. Metabolomics is a post-genomics study that analyzes metabolic profile in all biological samples. Gas chromatography-mass spectrometry (GC-MS) is a powerful technique that detects volatile components. In this study we evaluate the metabolite profile of serum with a simple method for derivatization. The metabolic profile of serum taken from 16 rats was analyzed using GC-MS after protein precipitation with acetonitrile. Then, it was derivatized with a method requiring the less number and volume of materials. Acquisition chromatograms were pre-processed and analyzed using National Institute of Standards and Technology (NIST) library. We detected 35 metabolites with probability of >60% perfect match according to spectrum fragments analysis by NIST library.This study represents the metabolome profile of serum instead the change of metabolites that can be evaluated by a simple derivatization method.


metabolomics, rat, serum, GC-MS


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DOI: https://doi.org/10.22037/jps.v9i1.16927


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