Noise is considered as one of the harmful factors at industrial environments. It affects human health in different aspects including harmful influences on auditory, vision, neurological and psychiatric systems, hormones, physiological and mental systems. Paying attention to the importance of hormonal effects on human body seems to possess crucial importance. The aim of the present study is to investigate the effects of noise exposure on variation of serum levels of glucose and cortisol at Syrian adult male mouse. 36 Syrian male adult mice were randomly categorized into two experiment and control groups. Garlic powder was added to the mice pelleted food meal (equal to 1% of their daily diet) to study its effect on the cortisol and glucose serum levels. The mice pelleted food contains starch, glucose, isolated soya protein, etc., which are the rich resources of A, C, B1, B2 and B6 vitamins. Streptozotocin (60mg/kg) was injected to the mice at experiment group which were under noise exposure with intensity of 90dB in frequency range of 700-5700Hz. Noise were generated using CoolEdit software and distributed to the fabricated box for 30 days (8h/d). Fasting cortisol and glucose serum levels were measured for mice of both groups 24h after finishing the exposure period. Results indicated that the glucose serum level in non-diabetic and diabetic subjects under noise exposure had significant difference (p<0.002) with the subjects at control group. Also, cortisol level in non-diabetic and diabetic subjects under noise exposure indicated significant difference (p<0.002) with the subjects at control group. Exposure to noise with intensity of 90dB at frequency range of 700-5700Hz increases the glucose and cortisol serum levels.